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Identifikasi Immunohistokimiawi Desmin dan Vimentin dalam Sel Otot Skelet Ayam Kedu Cemani (Galllus gallus domesticus) Budipitojo, Teguh; Wihadmadyatami, Hevi; -, Ariana; Musana, Dewi K.
Journal of Biota Biota Volume 19 Nomor 1 Tahun 2014
Publisher : PBI Yogyakarta

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AbstractThe two sub units of intermediate filaments, desmin and vimentin, are found in most cells of mesenchymal origin. The content and distribution of desmin in mature skeletal muscle have been known, but for vimentin has been a subject of disagreement. The present study was aimed to clarify the presence of desmin and vimentin immunoreactivities in the skeletal muscle fibers of Kedu Cemani chickens (Gallus gallus domesticus) by immunohistochemistry methods of avidin-biotin-complexs. Samples of muscle tissues were obtained from pectorales mayor, biceps brachii, and biceps femoris of five adult Kedu Cemani chickens (Gallus gallus domesticus). Immunohistochemical staining results showed that desmin immunoreactivities were detected in Z disc of myofibers, but not vimentin. The results stimulate further exploration on characteristics of Kedu Cemani chickens, especially in terms of the distribution of other intermediate filaments and neuroendocrine cells in a variety of organs system.Key words: Kedu chicken, skeletal muscle, desmin, vimentin, immunohistochemistryAbstrakVimentin dan desmin merupakan subunit filamen intermedia dan dapat ditemukan dalam sebagian besar sel mesenkimal. Keberadaan dan distribusi desmin dalam sel otot skelet dewasa/masak telah diketahui, namun keberadaan dan distribusi vimentin pada sel yang sama masih menjadi perdebatan. Penelitian ini bertujuan mengklarifikasi keberadaan immunoreaktifitas desmin dan vimentin dalam serabut otot skelet ayam kedu cemani dengan metode immunohistokimia komplek avidin-biotin. Penelitian dilakukan menggunakan jaringan otot yang meliputi otot pectorales mayor, biceps brachii, dan biceps femoris dari 5 ekor ayam kedu cemani (Gallus gallus domesticus). Pengamatan terhadap immunoreaktifitas desmin dan vimentin dalam serabut otot normal ayam kedu cemani dewasa dengan menggunakan antibodi monoklonal terhadap desmin dan antibodi poliklonal terhadap vimentin memperoleh hasil bahwa immunoreaktifitas desmin terdeteksi pada diskus Z serabut otot skelet, namun immunoreaktifitas vimentin tidak terdeteksi. Hasil penelitian memunculkan dorongan untuk menggali lebih lanjut ciri-ciri khusus ayam kedu cemani, terutama dalam hal distribusi filamen intermedia dan jenis neuroendokrin lainnya pada berbagai sistem organ tubuh.Kata kunci: Ayam kedu, otot skelet, desmin, vimentin, immunohistokimia

Platelet reactive alloantibodies responsible for immune thrombocytopenia in Malay population Armawai, Mohd I.; Hassan, Afifah; Wihadmadyatami, Hevi; Santoso, Sentot
Medical Journal of Indonesia Vol 23, No 3 (2014): August
Publisher : Faculty of Medicine Universitas Indonesia

Background: Alloantibodies against human platelet alloantigens (HPAs) are responsible for the development of platelet transfusion refractoriness (PTR) in patients receiving random platelets and bleeding disorder in babies with fetal neonatal alloimmune thrombocytopenia (FNAIT). Recently, our results based on the analysis of the allelic distribution of HPAs indicated that immunization may occur among Malay. In this study, we sought to analyze the frequencies of platelet reactive alloantibodies responsible for FNAIT and PTR in Malaysia.Methods: Sera from suspected FNAIT (n = 295) and PTR (n = 74) were collected in five years period (2008-2013) and tested for the presence of platelet reactive antibodies by the use of antigen capture assay.Results: In 5/74 (5.41%) platelet specific antibodies against HPA-2b (n = 1), HPA-5a (n = 1), HPA-5b (n = 1), HPA-15b (n = 2) could be identified in our PTR cohort. In FNAIT cohort, platelet specific alloantibodies could be detected in 18 sera (6.10%) consisting anti-HPA-1a (n = 1), anti-HPA-3a (n = 3), anti-HPA-5a (n = 6), anti-HPA-5b (n = 6), anti-HPA-15a (n = 1), and anti-HPA-15b (n = 1).Conclusion: Our study indicates that anti-HPA-3, -HPA-5 and -HPA-15 antibodies seems to be the most platelet specific antibodies involved in FNAIT and PTR cases in Malaysian population. Since similar HPA allelic distribution among Malaysian and Indonesian populations have been observed, immunization against these three HPA systems are expected to be the most potential risk of alloimmune mediated platelet disorders in Indonesia.

Identifikasi Immunohistokimiawi Desmin dan Vimentin dalam Sel Otot Skelet Ayam Kedu Cemani (Galllus gallus domesticus) Budipitojo, Teguh; Wihadmadyatami, Hevi; -, Ariana; Musana, Dewi K.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 19, No 1 (2014): February 2014
Publisher : Universitas Atma Jaya Yogyakarta

AbstractThe two sub units of intermediate filaments, desmin and vimentin, are found in most cells of mesenchymal origin. The content and distribution of desmin in mature skeletal muscle have been known, but for vimentin has been a subject of disagreement. The present study was aimed to clarify the presence of desmin and vimentin immunoreactivities in the skeletal muscle fibers of Kedu Cemani chickens (Gallus gallus domesticus) by immunohistochemistry methods of avidin-biotin-complexs. Samples of muscle tissues were obtained from pectorales mayor, biceps brachii, and biceps femoris of five adult Kedu Cemani chickens (Gallus gallus domesticus). Immunohistochemical staining results showed that desmin immunoreactivities were detected in Z disc of myofibers, but not vimentin. The results stimulate further exploration on characteristics of Kedu Cemani chickens, especially in terms of the distribution of other intermediate filaments and neuroendocrine cells in a variety of organs system.Key words: Kedu chicken, skeletal muscle, desmin, vimentin, immunohistochemistryAbstrakVimentin dan desmin merupakan subunit filamen intermedia dan dapat ditemukan dalam sebagian besar sel mesenkimal. Keberadaan dan distribusi desmin dalam sel otot skelet dewasa/masak telah diketahui, namun keberadaan dan distribusi vimentin pada sel yang sama masih menjadi perdebatan. Penelitian ini bertujuan mengklarifikasi keberadaan immunoreaktifitas desmin dan vimentin dalam serabut otot skelet ayam kedu cemani dengan metode immunohistokimia komplek avidin-biotin. Penelitian dilakukan menggunakan jaringan otot yang meliputi otot pectorales mayor, biceps brachii, dan biceps femoris dari 5 ekor ayam kedu cemani (Gallus gallus domesticus). Pengamatan terhadap immunoreaktifitas desmin dan vimentin dalam serabut otot normal ayam kedu cemani dewasa dengan menggunakan antibodi monoklonal terhadap desmin dan antibodi poliklonal terhadap vimentin memperoleh hasil bahwa immunoreaktifitas desmin terdeteksi pada diskus Z serabut otot skelet, namun immunoreaktifitas vimentin tidak terdeteksi. Hasil penelitian memunculkan dorongan untuk menggali lebih lanjut ciri-ciri khusus ayam kedu cemani, terutama dalam hal distribusi filamen intermedia dan jenis neuroendokrin lainnya pada berbagai sistem organ tubuh.Kata kunci: Ayam kedu, otot skelet, desmin, vimentin, immunohistokimia

THE EFFICACIES OF BANANA STEM EXTRACT AS A CANDIDATE OF COCCIDIOSTAT AGAINST RABBIT EIMERIA STIEDAIO OCYSTS: AN IN VITRO ANALYSIS Indrasanti, Diana; Indradji, Mohandas; Hastuti, Sri; Wihadmadyatami, Hevi; Ismoyowati, Ismoyowati
ANIMAL PRODUCTION Vol 17, No 3 (2015): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

The objective of this research was to investigatethe ability of banana stem (Musa paradisiaca) to inhibitsporulation of Eimeria stiedaioocystsderived fromrabbit by in vitroanalysis.Analyze the active substance proximate analysis and active substancesin this research were performed too. Banana stem extract were used in this experiment andsulfaquinoxalline(Coxy ®)was run as acontrol. The Eimeria stiedaioocystswere incubated prior the presence of different concentration from banana stem extract 0%, 1%, 2%, 4%, 8%for 1, 2 and 3 daysat 26°C. In addition,Factorial patterned Completely Randomized Design (CRD) with five replicates wasapplied on the experiment. Result analysis was performed by using Analysis of Variance and following by Honestly Significant Difference (HSD) post hoc test. Here, we identified that banana stem extract contain different type of active substance such as tannin, saponin, and alkaloid. Banana stem extract significantly affected the oocysts sporulation included the amount of sporulatedoocysts (P<0.01), unsporulatedoocysts (P<0.01), and transformed oocysts (P<0.01). In conclusion banana stem could inhibit the development of Eimeria stiedaioocysts on in vitroexperiment. HSD test showed that the optimum potential efficacy of banana stem toinhibit sporulation was at 4% and 8% concentration during three days incubation.

Isolasi dan identifikasi ekspresi protein rekombinan granule-1 (GRA-1) Toxoplasma gondii isolat lokal Hevi Wihadmadyatami; Rini Widayanti; Wayan Tunas Artama
Jurnal Sain Veteriner Vol 29, No 2 (2011): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Toxoplasmosis is a diseases caused by intracellular protozoa called Toxoplasma gondii.

Identifikasi Sifat dan Distribusi Sel Endokrin Ghrelin di Lambung Tikus (Rattus norvegicus): Studi Immunohis-Tokimia pada Kondisi Obesitas Teguh Budipitojo; Hevi Wihadmadyatami; Ganies Riza Aristya
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 1 (2016): June
Publisher : Universitas Gadjah Mada

Obesity is one of major nutritional problems in the world. Obesity is very dangerous, especially when concentrated in the abdomen, because it is closely linked to various diseases such as diabetes, hypertension, heart disease, which can to causing death. This study aims to identify the nature and distribution of ghrelin on gastric endocrine cells in the obese rat (Rattus norvegicus) by using immunohistochemical techniques. The results will strengthen the understanding of the role and function of ghrelin as an alternative therapeutic target on obesity. The research used gastric tissues of ten obese and control rats which were stained with avidin-biotin-peroxidase complex method of immunohistochemistry. The results showed the existence of two types of ghrelin-producing cells (open and closed types) on the gastric mucosa of control rats, and only one type of ghrelin producing cells (open type) in obese rats. The intensity of ghrelin immunoreactive positive cells was detected weak in obese rats, but very strong in control rats. Ghrelin endocrine cells mainly distributed in the basal part of the gastric mucosa of the fundus parts, with a very small number in obese rats, but highly abundant in control rats. This study confirmed the decrease of the ghrelin synthesis and secretion in obese rat (Rattus norvegicus) at the cellular level. The decrease of ghrelin synthesis is characterized by a reduction on the number of ghrelin producing cells, the disappearance of the close type of ghrelin producing cells, and the low activity of protein synthesis in the ghrelin producing cells. Ghrelin endocrine cells distributed mainly in the basal part of the gastric mucosa, especially in the fundus parts.

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