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Body Weight, Oocyte Elimination and Blood Profile of Rabbit After Challenge Test Using Eimeria stiedai Indrasanti, Diana; Indradji, Mohandas; Hastuti, Sri; Fitriyani, Nur; Munawaroh, Sitie; Hikmawati, Nita; Irawan, Desika Indra
ANIMAL PRODUCTION Vol 20, No 1 (2018)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (749.469 KB) | DOI: 10.20884/1.jap.2018.20.1.621

Abstract

The objective of the research was to investigate body weight, oocyte elimination and blood profile of rabbits infected with various doses of Eimeria stiedai isolates. The observed rabbits’ blood profile included erythrocyte, hemoglobin, hematocrit, leucocyte, thrombocyte, total protein plasma (TPP) and fibrinogen. Twenty-five male New Zealand White rabbits aged 3 months and weighed approximately 2 kg were provided with pellet and boiled drinking water and Eimeria stiedai isolates. The experiment used Completely Randomized Design to analyze 5 treatments with five replicates. The examined variables included D0: Infection 0 (control of infection without challenge test), D1: Infection 101 with challenge test 103, D2: infection 102 with challenge test 103, D3: infection 103 with challenge test 103, D4: infection 0 with challenge test 103 (control of infection). Data were subject to analysis of variance followed by Honestly Significant Difference Test (HSD). Analysis of Variance result showed that there was no significant difference on body weight, oocyte elimination and blood profile including erythrocyte, hemoglobin, hematocrit, leucocyte, thrombocyte, and fibrinogen. However, total protein plasma (TTP) was significantly different at 5% HSD. It can be concluded that challenge test with Eimeria stiedai has not been used as an alternative in increasing rabbits’ body immune against coccidiosis infection.
THE EFFICACIES OF BANANA STEM EXTRACT AS A CANDIDATE OF COCCIDIOSTAT AGAINST RABBIT EIMERIA STIEDAIO OCYSTS: AN IN VITRO ANALYSIS Indrasanti, Diana; Indradji, Mohandas; Hastuti, Sri; Wihadmadyatami, Hevi; Ismoyowati, Ismoyowati
ANIMAL PRODUCTION Vol 17, No 3 (2015): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (741.083 KB) | DOI: 10.20884/1.anprod.2015.17.3.503

Abstract

The objective of this research was to investigatethe ability of banana stem (Musa paradisiaca) to inhibitsporulation of Eimeria stiedaioocystsderived fromrabbit by in vitroanalysis.Analyze the active substance proximate analysis and active substancesin this research were performed too. Banana stem extract were used in this experiment andsulfaquinoxalline(Coxy ®)was run as acontrol. The Eimeria stiedaioocystswere incubated prior the presence of  different concentration from banana stem extract  0%, 1%, 2%, 4%, 8%for 1, 2 and 3 daysat 26°C. In addition,Factorial patterned Completely Randomized Design (CRD) with five replicates wasapplied on the experiment. Result analysis was performed by using Analysis of Variance and following by Honestly Significant Difference (HSD) post hoc test. Here, we identified that banana stem extract contain different type of active substance such as tannin, saponin, and alkaloid. Banana stem extract significantly affected the oocysts sporulation included the amount of sporulatedoocysts (P<0.01), unsporulatedoocysts (P<0.01), and transformed oocysts (P<0.01). In conclusion banana stem could inhibit the development of Eimeria stiedaioocysts on in vitroexperiment. HSD test showed that the optimum potential efficacy of banana stem toinhibit sporulation was at 4% and 8% concentration during three days incubation.
Levels of Fibrinogen as Predictor of Severity of Coccidiosis Infection in Rabbit Livestock Indradji, Mohandas; Indrasanti, Diana; Hartono, Madi; Sufiriyanto, Sufiriyanto; Yuwono, Endro; Samsi, Muhamad
JURNAL ILMIAH PETERNAKAN TERPADU Vol 9, No 2 (2021)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v9i2.p186-193

Abstract

The purpose of this study was to make a linear model of the leukogram variable with the number of oocysts in cases of coccidiosis infection in rabbits. The infection response in illness of disease course and will be linearly proportional to the rate of severity that occurs. Blood samples obtained from 91 rabbits were examined to determine the value of their lekogram component, as well as 91 rabbit feces were examined to determine the number of livestock can be described in the dynamics of the components of white blood cells (leukocytes), the increased response to infection clinically and haematologically will run parallel to the coccidia oocysts. The quantitative data from the count of oocysts with lekogram values were analyzed using a multivariate regression model, to determine the best linear model that could describe the severity of coccidiosis infection with the lekogram value, with the number of oocysts as the dependent variable and the lecogram values as the independent variable, the best model of linearity can be used to predict disease severity. Of all the lekogram variables analyzed, the amount of total fibrinogen had a significant effect (P <0.05) on the severity of Coccidiosis, with the linear formula Y = 2.7 + Fibrinogen Value, so that the fibrinogen value could be used to predict the severity of Coccidiosis in rabbits.