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Antioxidant activity, determination of total phenolic and flavonoid content of Muntingia calabura L. Extracts Puspitasari, Anita Dwi; Wulandari, Ririn Lispita
Pharmaciana Vol 7, No 2 (2017): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Muntingia calabura L. leaves were contained alkaloids, saponins, phenolic, flavonoids, and tannin. Phenolic and flavonoids compound had antioxidant activity. The present study aims to determine the antioxidant activity, determination of total phenolic and flavonoid content of ethanol extract of Muntingia calabura L. leaves and its fractions. Muntingia calabura L. leaves were macerated with 96% ethanol and then evaporated until ethanol extract was obtained. The ethanol extract was dissolved into water and then partitioned with n hexane and ethyl acetate to obtain the n hexane fraction, ethyl acetate fraction and water fraction. Determination of antioxidant activity of extracts and fractions was performed by free radical capture measurement method by 1,1-diphenyl-2-picrylhydrazyl (DPPH) in vitro. Vitamin C was used as a positive control with IC50 value of Â Â Â Â Â Â Â 25.74 Âµg/mL. The total phenolic and flavonoid content were determined using the colorimetric method using standard gallic acid for total phenolics and standard quercetin for flavonoid content. The results showed that ethyl acetat fraction showed the highest antioxidant activity with IC50 79.37 Î¼g/mL value compared to n hexane fraction (101.36 Î¼g/mL), ethanol extract (126.47 Î¼g/mL), and water fraction (129.85 Î¼g/mL). The ethyl acetate fraction contains the highest total phenolic of 510.57 mg GAE/g extract compared to ethanol extract (311.10 mg GAE/g extract), water fraction (292.74 mg GAE/g) extract, and n hexane fraction (103.95 mg GAE/g extract). The ethyl acetate fraction contained the highest flavonoid content of 76.32 mg QE/g extract compared to ethanol extract (39.63 mg QE/g extract), water fraction (14.29 mg QE/g extract) and n hexane fraction (3.30 mg QE/g extract). Total phenolic and total flavonoid levels were positively correlated with antioxidant activity

Antioxidant activity, determination of total phenolic and flavonoid content of Muntingia calabura L. Extracts Anita Dwi Puspitasari; Ririn Lispita Wulandari
Pharmaciana Vol 7, No 2 (2017): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Muntingia calabura L. leaves were contained alkaloids, saponins, phenolic, flavonoids, and tannin. Phenolic and flavonoids compound had antioxidant activity. The present study aims to determine the antioxidant activity, determination of total phenolic and flavonoid content of ethanol extract of Muntingia calabura L. leaves and its fractions. Muntingia calabura L. leaves were macerated with 96% ethanol and then evaporated until ethanol extract was obtained. The ethanol extract was dissolved into water and then partitioned with n hexane and ethyl acetate to obtain the n hexane fraction, ethyl acetate fraction and water fraction. Determination of antioxidant activity of extracts and fractions was performed by free radical capture measurement method by 1,1-diphenyl-2-picrylhydrazyl (DPPH) in vitro. Vitamin C was used as a positive control with IC50 value of Â Â Â Â Â Â Â 25.74 Âµg/mL. The total phenolic and flavonoid content were determined using the colorimetric method using standard gallic acid for total phenolics and standard quercetin for flavonoid content. The results showed that ethyl acetat fraction showed the highest antioxidant activity with IC50 79.37 Î¼g/mL value compared to n hexane fraction (101.36 Î¼g/mL), ethanol extract (126.47 Î¼g/mL), and water fraction (129.85 Î¼g/mL). The ethyl acetate fraction contains the highest total phenolic of 510.57 mg GAE/g extract compared to ethanol extract (311.10 mg GAE/g extract), water fraction (292.74 mg GAE/g) extract, and n hexane fraction (103.95 mg GAE/g extract). The ethyl acetate fraction contained the highest flavonoid content of 76.32 mg QE/g extract compared to ethanol extract (39.63 mg QE/g extract), water fraction (14.29 mg QE/g extract) and n hexane fraction (3.30 mg QE/g extract). Total phenolic and total flavonoid levels were positively correlated with antioxidant activity

EFEK ANTI JAMUR TRICHOPHYTON MENTAGROPHYTES LOSION EKSTRAK ETANOL BUAH MENGKUDU MENTAH (MORINDA CITRIFOLIA L.) SECARA IN VIVO Ririn Lispita Wulandari; Nurahmah Hidayati; Maulita Cut Nuria
Prosiding SNST Fakultas Teknik Vol 1, No 1 (2019): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI 10 2019
Publisher : Prosiding SNST Fakultas Teknik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.026 KB)

Losion ekstrak etanol buah mengkudu mentah (EEBMM) dengan emulgator PGA 20% pada konsentrasi ekstrak 12 mg/mL terbukti mempunyai aktivitas anti jamur terhadap Trichophyton mentagrophytes secara in vitro (persentase inhibisi 100%). Penelitian ini bertujuan untuk menguji efek anti jamur losion EEBMM secarain vivo pada kelinci yang diinfeksi Trichophyton mentagrophytes. Penelitian ini menggunakan Posttest Only Control Group Design. Ekstrak buah mengkudu mentah diperoleh dengan cara perkolasi dan losion dibuat dengan emulgator PGA 20% yang mengandung tiga konsentrasi EEBMM berbeda yaitu 12, 18, 27 mg/mL. Efek anti jamurlosion dengan konsentrasi EEBMM 12 mg/mL (P1), 18 mg/mL (P2) dan 27 mg/mL (P3), losion tanpa eksrak (K-) dan krim ketokonazol 2 % (K+) diuji secara in vivo terhadap kelinci yang diinfeksi jamur Trichophyton mentagrophytes dan diobati selama 16 hari. Parameter yang diukur berupa data skor luka infeksi, lalu dianalisa statistik menggunakan Uji Kruskal Wallis dan Mann Whitney. Hasil penelitian menunjukkan bahwa losion EEBMM memiliki efek anti jamur pada kelinci yang diinfeksi Trichophyton mentagrophytes secara in vivo dibandingkan dengan kontrol negatif mulai hari ke-9 untukkonsentrasi 18 mg/mL dan 27 mg/mLsertahari ke-11 untuk konsentrasi 12 mg/mL.Kata kunci:Losion ekstrak etanol buah mengkudu mentah, PGA, Trichophyton mentagrophytes, in vivo.

Sand Granules Ekstrak Etanol Daun Kirinyuh (Eupatorium inulifolium Kunth.) sebagai Larvasida Alami Pemberantas Demam Berdarah Dengue Puji Hartati; Yance Anas; Ririn Lispita Wulandari
Jurnal Pharmascience Vol 7, No 1 (2020): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v7i1.8067

ABSTRAK Penelitian sebelumnya telah mengungkap efek larvasida ekstrak etanol daun kirinyuh (Eupatorium inulifolium Kunth.) terhadap larva nyamuk Aedes aegypti. Ekstrak ini selanjutnya dapat dikembangkan dalam bentuk sediaan sand granules sehingga mudah digunakan sebagai larvasida alami. Penelitian ini bertujuan untuk mendapatkan formula sand granules ekstrak etanol daun Eupatorium inulifolium Kunth (EEDE) yang memenuhi standar karakteristik fisik granul dan menguji efek larvasidanya pada larva nyamuk Aedes aegypti. Sand granules EEDE dibuat dengan metode granulasi basah dengan variasi konsentrasi kollidon (1%-5%), explotab (1%-3%) dan sacharum lactis (78,44%-84,44%). Karakteristik fisik berbagai formula Sand granules EEDE yang diuji adalah kecepatan alir dan sudut diam. Uji efek larvasida dilakukan pada 25 ekor larva instar III nyamuk Aedes aegypti selama 24 jam dengan replikasi sebanyak 5 kali. Hasil penelitian menunjukkan bahwa semua formula Sand granules EEDE (F1-F5) memenuhi standar karakteristik fisik dengan kecepatan alir (16,69-19,35) g/detik dan sudut diam granul 32,94°-35,76°. F1-F5 juga memiliki efek larvasida terhadap larva nyamuk Aedes aegypti dengan persentase kematian larva dalam 24 jam sebesar (33,0-39,2) %. Akan tetapi, efek larvasida tersebut lebih kecil dibandingkan dengan EEDE 670 ppm (49,6%) dan Temefos® 0,01 ppm (100,0%). Hasil penelitian ini juga menyimpulkan bahwa proses pembuatan sand granules akan menyebabkan penurunan efek larvasida EEDE. Kata Kunci: Daun kirinyuh, efek larvasida, karakteristik fisik, sand granules ABSTRACT Previous studies reported Eupatorium inulifolium Kunth. leaves ethanol extract (ELEE) have a larvicidal effect on Aedes aegypti larvae. This extract can be developed into sand granules formulation, making it easy to use as a natural larvicidal. This study means to obtain the ELEE sand granules formula that meets the granule's physical characteristics standard, and asses it's a larvicidal effect on Aedes aegypti larvae. Sand granules of ELEE produced with the wet granulation method. The differences between formulas are variations of Kollidon (1%-5%), Explotab (1%-3%), and Saccharum Lactis (78.44%-84.44%) concentration. The physical characteristics of the various KLEE sand granules formulas observed are flow rate and angle of repose. The assay of the ELEE larvicidal effect was carried out on twenty-five Aedes aegypti Instar III larvae for 24 hours with five replications. The results showed that all ELEE sand granules formulas (F1-F5) met the physical characteristics standard, with a flow rate (16.69-19.35) g/sec and an angle of repose 32.94 °-35.76°. F-F5 also had larvicidal effects on Aedes aegypti larvae, with larval mortality within 24 hours (33.0-39.2) %. However, their larvicidal effect is slight compared to ELEE 670 ppm (49.6%) and Temefos® 0.01 ppm (100.0%). This study also concludes that the process of sand granules preparation will diminish the larvicidal effects of ELEE. Keywords: Kirinyuh leaves, larvacidal effect, physical characteristics, sand granules

Aktivitas Antioksidan dan Penetapan Kadar Flavonoid Total Ekstrak Etil Asetat Daun Kersen (Muntingia calabura) Anita Dwi Puspitasari; Ririn Lispita Wulandari
Jurnal Pharmascience Vol 4, No 2 (2017): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v4i2.5770

Daun kersen (Muntingia calabura ) secara empiris di masyarakat digunakan untuk mengobati berbagai macam penyakit. Salah satu potensi yang dimiliki dari daun kersen adalah sebagai antioksidan. Senyawa aktif yang dimiliki oleh daun kersen yang memiliki aktivitas antioksidan diantaranya adalah fenolik, flavonoid, dan alkaloid. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan dan penetapan kadar flavonoid total ekstrak etil asetat daun kersen (Muntingia calabura). Pembuatan ekstrak etil asetat daun kersen menggunakan metode maserasi dengan pelarut etil asetat. Analisis aktivitas antioksidan menggunakan metode DPPH (1,1-difenil-2-pikrilhidrazil) dengan pembanding vitamin C. Penetapan kadar flavonoid total menggunakan metode spektrofotometri Ultra Ungu-Sinar Tampak dengan pembanding Quersetin. Hasil penapisan fitokimia menunjukkan adanya kandungan senyawa alkaloid, saponin, fenolik, flavonoid, dan tannin. Hasil pengujian aktivitas antioksidan ekstrak etil asetat daun kersen dengan nilai IC50 sebesar 53,25 µg/mL dengan penbanding vitamin C (IC50 25,74 µg/mL ). Hasil penetapan kadar flavonoid total sebesar 93,21 mgEQ/g Ekstrak. Kata kunci: antioksidan, flavonoid total, muntingia calabura

ANTITHROMBOCYTOPENIA ACTIVITY OF PEANUT SHELL (Arachis hypogea L.) EXTRACT AND INFUSA ON HEPARIN INDUCED BALB/C MICE risha fillah fithria; Ririn Lispita Wulandari; Devi Nisa Hidayati; Pia Annisa; Nilam Eka Putri
Jurnal Tumbuhan Obat Indonesia Vol 12 No 1 (2019): Jurnal Tumbuhan Obat Indonesia
Publisher : Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional

ABSTRACT Certain group of people empirically use peanut shell stew in the treatment of thrombocytopenia due to dengue fever. The active compounds suspected to play a role in the activity of thrombocytopenia from peanut shell are flavonoids. Flavonoids can be extracted using heat and cold methods. This study aims to determine the antithrombocytopenia activity of peanut shell extract and infusa on heparin induced Balb/C mice. This study is an experimental research with randomized matched pre and postest control group design. Peanut shell extraction was done in a cool way by maseration using 70% ethanol solvent, and hot way by infundation using aquadest solvent. Forty-eight Balb/C mice were divided into 8 groups consisting of control group CMC Na 0.5 mL/20gBW/day, control group aquadest 0,5mL/20gBW/day, 3 groups of peanut shell ethanol extract (0.019, 0.038, and 0.076) g/20gBW/day, and 3 groups of peanut shell infusa (0.026, 0.052; 0.104) g/20gBW/day. All treatments were given orally. The decrease of platelet count in Balb/C mice using 26 UI/20gBW subcutan heparin induction. The measurement of the platelet count is performed by taking blood samples in the lateral veins of the tail. Platelet counts data before and after treatment was tested with dependent T-test. Differences in platelet increases across the groups were tested by 2 way anova and continued with a LSD test with 95% confidence level. The results showed that peanut shell ethanol extract had better antitrombocytopenia activity than peanut shell infusa.

PEMANFAATAN DAUN SIRSAK UNTUK PENCEGAHAN DAN PENGOBATAN ALTERNATIF PENYAKIT KANKER Ririn Lispita Wulandari
ABDIMAS UNWAHAS Vol 1, No 1 (2016)
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31942/abd.v1i1.1705

Daun sirsak merupakan tanaman yang memiliki khasiat sebagai antikanker. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pengetahuan dan ketrampilan warga masyarakat tentang pentingnya penggunaan daun sirsak (Annona muricata) sebagai obat herbal untuk pencegahan dan pengobatan alternatif penyakit kanker. Kegiatan ini dilaksanakan di Dusun Jambon RT 04/ RW 03, Kelurahan Ngadirgo, Kecamatan Mijen, Semarang. Bentuk kegiatannya yaitu memberikan penyuluhan (ceramah dan tanya jawab) dan pelatihan. Sasaran kegiatan adalah ibu-ibu PKK RT 04/ RW 03, Kelurahan Ngadirgo, Kecamatan Mijen. Langkah-langkah kegiatan meliputi, persiapan, pelaksanaan, dan evaluasi. Para ibu-ibu PKK mendapatkan penjelasan mengenai khasiat daun sirsak, cara pengolahan, serta pemanfaatannya yang baik dan benar, dan melakukan pelatihan cara membuat teh daun sirsak. Evaluasi telah dilakukan sesudah kegiatan pengabdian..Kata Kunci: Daun sirsak, kanker, Kelurahan Ngadirgo

PEMANFAATAN TANAMAN OBAT KELUARGA (TOGA) UNTUK PENGOBATAN DIABETES MELITUS Ririn Lispita Wulandari
ABDIMAS UNWAHAS Vol 3, No 1 (2018)
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31942/abd.v3i1.2235

TOGA merupakan tanaman obat keluarga yang memiliki manfaat bagi kesehatan manusia, tanpa kecuali termasuk pengobatan diabetes melitus. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pengetahuan dan ketrampilan warga masyarakat tentang pentingnya TOGA sebagai obat herbal untuk pencegahan dan pengobatan alternatif penyakit diabetes melitus. Kegiatan ini dilaksanakan di.RW XVI Kelurahan TambakAji. Bentuk kegiatannya yaitu penyuluhan dengan metode ceramah dan diskusi. Sasaran kegiatan adalah ibu-ibu PKK RW XVI, Kelurahan Tambak Aji. Langkah-langkah kegiatan meliputi persiapan, pelaksanaan, dan evaluasi. Setelah kegiatan pengabdian ini dilaksanakan, para ibu PKK RW XVI Kelurahan TambakAji telah mengetahui khasiat jenis-jenis TOGA dan cara pemanfaatannya untuk kesehatan, khususnya pengobatan diabetes melitus.Kata Kunci: TOGA, Diabetes Melitus, RW XVI, Kelurahan Tambak AjiPENDAHULUAN

FORMULASI DAN EFEK SALEP EKSTRAK ETANOL DAUN JAMBU BIJI (Psidium guajava,L.) DENGAN BASIS HIDROKARBON TERHADAP LUKA SAYAT PADA TIKUS JANTAN GALUR WISTAR Ririn Lispita Wulandari; Elya Zulfa; Dwara Andriani Astuti
Jurnal Ilmu Farmasi dan Farmasi Klinik Vol 16, No 02 (2019): Jurnal Ilmu Farmasi & farmasi Klinik Vol 16 No 02 Desember 2019
Publisher : Universitas Wahid Hasyim Semarang

ABSTRACT The ethanol extract of guava leaves (EEGL) is proven to heal various types of wounds.This study aims to determine the physicochemical characteristics of the EEGL ointment with a hydrocarbon base formula and examine the effect of the ointment against cut wounds on wistar male rat. The EEGL was obtained by maceration using 70% ethanol solvent. The ointment made 3 formulas with a ratio of hydrocarbon base composition (cera alba:vaselin album) namely F1 (2.5:92.5)%; F2 (5:90)%; F3 (7.5:87.5)%. The physicochemical characteristics of all ointments examined included organoleptic, homogeneity, pH, viscosity, spreadability, and adhesion. Organoleptic, homogeneity and pH data were analyzed descriptively, while viscosity, spreadability and adhesion data were analyzedstatistically. Testing the effect of all ointment formulas on the wound was done by applying 0.5 gram 2x a day for 9 days compared to wound control without treatment and positive control used commercial product.The parameter of effect measured was the length of the cut wound on the 9th day, then analyzed with the Kruskall Wallis test followed by the Mann Whitney test.The physicochemical characteristics of EEGL ointment (F1, F2, F3) are known that all ointment formulas are brown in color, typical of guava, homogeneous, pH according to skin pH, viscosity and adhesion are significantly different, while the spredability is not significant.The EEGL ointment had an effect on the cut wound of the male wistar rats, ie the length of the wound on the 9th day was reduced and compared to wound controlwas differentsignificantly. Keywords :Guava leaves, ointment, cut of wound.

EFEK GASTROPROTEKTIF EKSTRAK ETANOL BUAH LABU KUNING (Cucurbita moschata (Duch.) Poir) PADA TIKUS JANTAN GALUR WISTAR YANG DIINDUKSI ASPIRIN Maulida Shofiatun; Ririn Lispita Wulandari
Jurnal Ilmu Farmasi dan Farmasi Klinik Vol 17, No 2 (2020): Jurnal Ilmu Farmasi & Farmasi Klinik Vol. 17 N0. 02 Desember 2020
Publisher : Universitas Wahid Hasyim Semarang

ABSTRACT Non-steroidal anti-inflammatory drugs (NSAIDs) can cause side effects of gastric ulcers. Based on previous research, it is known that pumpkin contains flavonoids which can protect the gastric mucosa. The aim of this study was to determine the gastroprotective effect of ethanol extract of Cucurbita moschata (Duch.) Poir fruit in male rats Wistar-induced by aspirin. The study used a randomized matched posttest only control group design. Cucurbita moschata (Duch.) Poir fruit extraction was carried out by ultrasonic method. A total of 24 rats were divided into 6 groups: normal control (CMC Na 0,5%; ulcer control (CMC Na 0,5%); positive control (ranitidine HCl 10 mg/kg BW); treatment group (Cucurbita moschata (Duch.) Poir fruit ethanol extract at dose of 100, 200, and 400 mg/kg BW). Treatments lasted for 14 days. One hour after administering the test solution on the 14th day, all rats were given 200 mg/kg BW of aspirin (except normal controls) orally, then fasted for 12 hours. On the 15th day, all rats were sacrificed, dissected and their stomachs were taken. Gastric histology preparations were made with hematoxylin-eosin staining, microscopically read, discussed the number and severity of ulcers. Both data were processed and analyzed by Kruskal Wallis and Mann Whitney (95% confidence level). The results showed that the administration of EEBLK doses of 100, 200, and 400 mg/kg BW resulted in a score of the number and severity of ulcers that were lower significantly than of ulcer controls (p

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