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All Journal Jurnal Ilmu Ternak dan Veteriner Animal Production : Indonesian Journal of Animal Production
D Yudhabuntara
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Campylobacter jejuni seroepidemiology in native chicken Rosyidi, Anwar; Budiharta, S; Asmara, W; Yudhabuntara, D
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 3 (2012)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.581 KB) | DOI: 10.14334/jitv.v17i3.706

Abstract

Campylobacter jejuni is responsible for about 90% of cases of Campylobacteriosis in humans with gastroenteritis. Healthy chickens can carry Campylobacter spp. in the intestinal tract. Efforts to reduce exposure to Campylobacteriosis by humans may be enhanced by knowledge of its prevalence in poultry. This study aimed to identify factors associated with seropositive response to C. jejuni in native chickens in Mataram. Detection of C. jejuni was accomplished using an immunochromatographic serological method. Association between Campylobacter jejuni seropositive response as the dependent variable with various independent variables was analyzed using χ² (Chi square) and Odds Ratio (OR). A total of 216 chicken samples were examined and 44 chicken owners were interviewed and their farms examined. Results showed the prevalence of serological response to C. jejuni in chicken samples to be as high as 35.6% and that as many as 70.5% of farms had affected chickens.  Age of the chicken was the variable most closely associated with incidence of seropositive response, birds older than 3 months more likely to be affected. Variables at the farm level associated with variation in seropositive response were cage type, cage floor material, and origin of drinking water, surface water sources being less desirable. Key Words: Campylobacter jejuni, Seroepidemiology, Native Chicken, Mataram
Phenotypic and Genotypic Detection of Campylobacter jejuni at Local Chicken and Chicken Meat Rosyidi, A; Budhiharta, S; Asmara, W; Yudhabuntara, D
ANIMAL PRODUCTION Vol 12, No 2 (2010): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.436 KB)

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The Objective of this study was to identify the existence of Campylobacter jejuni based on  phenotypic and genotypic characteristic in local chicken and chicken meats. Samples of local chicken  intestine and meat were tested  for the bacterial existence. Phenotypic examination was carried out by means of cultivation followed  by gram staining and biochemical tests. Genotypic examination was conducted by polymerase chain reaction (PCR) using genus specific16S rRNA gene at 816 bp  and membrane-associated protein A (mapA) gene  at 589 bp as Campylobacter jejuni species-specific gene. The result of phenotypic detection revealed the  existence of  Campylobacter spp as  gram negative, curved rod shape, oxidase positive,  urease negative and motile. Genotypic examination also indicated the existence of  bacteria using both primers. However, no Campylobacter jejuni detected from meat of the chickens. The results suggest that the method of PCR using a primer  detecting species-specific gene of Campylobacter jejuni gives a rapid and accurate detection of the bacteria as compared to that using phenotypic and biochemical test. Identification of Campylobacter spp from chicken meats  should be improved with enrichment method and sample collection. (Animal Production 12(2): 128-134 (2010)Key Words: Campylobacter jejuni, mapA gene, local chicken
Campylobacter jejuni seroepidemiology in native chicken Anwar Rosyidi; S Budiharta; W Asmara; D Yudhabuntara
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 3 (2012): SEPTEMBER 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.581 KB) | DOI: 10.14334/jitv.v17i3.706

Abstract

Campylobacter jejuni is responsible for about 90% of cases of Campylobacteriosis in humans with gastroenteritis. Healthy chickens can carry Campylobacter spp. in the intestinal tract. Efforts to reduce exposure to Campylobacteriosis by humans may be enhanced by knowledge of its prevalence in poultry. This study aimed to identify factors associated with seropositive response to C. jejuni in native chickens in Mataram. Detection of C. jejuni was accomplished using an immunochromatographic serological method. Association between Campylobacter jejuni seropositive response as the dependent variable with various independent variables was analyzed using χ² (Chi square) and Odds Ratio (OR). A total of 216 chicken samples were examined and 44 chicken owners were interviewed and their farms examined. Results showed the prevalence of serological response to C. jejuni in chicken samples to be as high as 35.6% and that as many as 70.5% of farms had affected chickens.  Age of the chicken was the variable most closely associated with incidence of seropositive response, birds older than 3 months more likely to be affected. Variables at the farm level associated with variation in seropositive response were cage type, cage floor material, and origin of drinking water, surface water sources being less desirable. Key Words: Campylobacter jejuni, Seroepidemiology, Native Chicken, Mataram
Co-Authors A Rosyidi Anwar Rosyidi S Budhiharta, S S Budiharta W Asmara