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ANTIFUNGAL EFFECTIVENESS OF CACAO BEAN SHELLS EXTRACT (Theobroma cocoa L.) ON TRICHOPHYTON RUBRUM GROWTH IN VITRO Chintya Mei Desia Hutasoit; Yuni Setyaningsih; Andri Pramono
Biomedika Vol 12, No 2 (2020): Biomedika Agustus 2020
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v12i2.10176

Abstract

ABSTRACTTrichophyton rubrum is the most common dermatophytosis etiology. The antifungal agent has several problems such as fungal resistance and therapy side effects. Cacao (Theobroma cocoa L.) bean shells extract contained flavonoids, alkaloids, and saponins which have an antifungal effect. This study aimed to test the in vitro cacao bean shells extract antifungal (especially: Trichophyton rubrum) effectiveness by agar well diffusion method. This was an experimental study with a post-test only control group design. This study used cacao bean shells extract concentration 25%, 50%, 75%, and 100%, ketokonazol as positive control, and aquadest as negative control. The average inhibition diameter of 25%, 50%, 75% and 100% cacao bean shells extract concentration were 10.65 mm, 18 mm, 26.92 mm, and 37.22 mm, respectively, while the ketoconazole inhibition diameter was 51.52 mm. Data were analyzed using the Kruskal-Wallis test and post hoc with Mann-Whitney. The results showed significant differences between each treatment group (p0.05). Cacao bean shells extract had an antifungal effect in inhibiting the growth of Tricophyton rubrum invitro.Keywords: Trichophyton rubrum, Cacao Bean Shells, Antifungal, Agar Well Diffusion Method,  ABSTRAKTrichophyton rubrum adalah penyebab paling umum dermatofitosis. Obat dermatofitosis (antijamur) memiliki beberapa masalah seperti resistensi dan efek samping terapi. Ekstrak cangkang biji kakao (Theobroma cocoa L.) mengandung flavonoid, alkaloid, dan saponin yang memiliki efek antijamur. Tujuan penelitian ini untuk menguji efektivitas antijamur (khususnya Trichophyton rubrum) ekstrak cangkang biji kakao secara in vitro dengan metode difusi agar. Penelitian ini merupakan penelitian eksperimental dengan post-test only control group design. Penelitian ini menggunakan konsentrasi ekstrak cankang biji kakao 25%, 50%, 75%, dan 100%, ketokonazol sebagai kontrol positif, dan aquadest sebagai kontrol negatif. Rata-rata diameter hambat konsentrasi 25%, 50%, 75% dan 100%  secara berturut-turut yaitu: 10,65 mm, 18 mm, 26,92 mm, dan 37,22 mm, sedangkan daya hambat ketoconazole adalah 51,52 mm. Data dianalisis menggunakan uji Kruskal-Wallis dan post hoc dengan Mann-Whitney. Hasil penelitian menunjukkan perbedaan yang signifikan antara masing-masing kelompok perlakuan (p 0,05). Ekstrak cangkang biji kakao memiliki efek antijamur dalam menghambat pertumbuhan Tricophyton rubrum invitro.Kata kunci: Trichophyton rubrum, Anti-jamur, Cangkang Biji Kaka, Metode Difusi Sumuran Agar
Hot Oil Exposure on Styrofoam and Black Crackle Bag Effect on Malondialdehyde MDA Level of White Rat (Rattus norvegicus) Wistar Strain Marcella; Kristina Simanjuntak; Yuni Setyaningsih
SCRIPTA SCORE Scientific Medical Journal Vol. 1 No. 2 (2020): SCRIPTA SCORE Scientific Medical Journal
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (871.998 KB) | DOI: 10.32734/scripta.v1i2.1235

Abstract

ABSTRACT This study purposed to determine the effect of hot oil exposure on styrofoam and black plastic bag on MDA levels in rats. The study was purely experimental, a sample of 28 Wistar rats, weight ±200gram, age 3 months, divided into 4 groups, treatment was given for 28 days. Negative control was given feed and drink; styrofoam group was given feed, drink, oil on styrofoam; the black plastic group was given feed, drink, oil on black plastic; positive control was given feed, drink, toluene mixed oil. On 14th and 28th-day blood was taken from the tail and examined for plasma MDA by spectrophotometer λ=532nm. One-way ANOVA shows there is an effect of hot oil exposure on styrofoam and black plastic on MDA levels on the 14th and 28th days (p=0.000). Post hoc showed the styrofoam group had a significant increase in MDA levels on 14th (p=0.003) and 28th (p=0.043) compared to negative controls, the black plastic group as well on 14th (p=0.000) and 28th (p=0.000). MDA levels between styrofoam and black plastic groups significantly different on 14th (p=0.012), but not on 28th (p=0.340). There was a significant MDA increase in the styrofoam group between 14th and 28th days (p=0.009), and in the black plastic group (p=0.031). This shows that hot oil exposure on styrofoam and black plastic cause cell oxidative stress. Keyword: Black Plastic Bag, MDA, Oxidative Stress, Styrofoam ABSTRAK Penelitian bertujuan mengetahui pengaruh paparan minyak panas pada stirofoam dan kresek hitam terhadap kadar MDA. Penelitian merupakan eksperimental murni, sampel 28 ekor tikus putih galur Wistar, berat badan ±200gram, usia 3 bulan, dibagi menjadi 4 kelompok, perlakuan selama 28 hari. Kontrol negatif diberi pakan biasa dan air minum; kelompok stirofoam diberi pakan, minum, dan minyak pada stirofoam; kelompok kresek hitam diberi pakan, minum, dan minyak pada kresek hitam; kontrol positif diberi pakan, minum, dan minyak dicampur toluene. Mengambil darah dari vena ekor pada hari ke-14 dan ke-28 lalu memeriksa MDA plasma dengan spektrofotometer λ=532 nm. Hasil One-way Anova menunjukkan peningkatan MDA kelompok stirofoam dan kresek hitam hari ke-14 dan ke-28 (p=0.000). Uji post hoc menunjukkan MDA kelompok stirofoam meningkat signifikan pada hari ke-14 (p=0.003) dan ke-28 (p=0.043) dibanding kontrol negatif, begitu juga kelompok kresek hitam hari ke-14 (p=0.000) dan ke-28 (p=0.000). Kadar MDA antara kelompok stirofoam dan kresek hitam berbeda signifikan pada hari ke-14 (p=0.012), tetapi tidak pada hari ke-28 (p=0.340). MDA berbeda bermakna antara hari ke-14 dan ke-28 pada kelompok stirofoam (p=0.009) dan kelompok kresek hitam (p=0.031). Hal ini menunjukkan paparan minyak panas pada stirofoam dan kresek hitam dapat menimbulkan stres oksidatif sel. Kata Kunci: Kresek Hitam, MDA, Stirofoam, Stres Oksidatif
RECOMMENDATIONS OF THE DEVELOPMENT OF INTERPROFESSIONAL EDUCATION IN A FACULTY OF MEDICINE Hikmah Muktamiroh; Agneta Irmarahayu; Yuni Setyaningsih
Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 8, No 2 (2019): Juli
Publisher : Asosiasi Institusi Pendidikan Kedokteran Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5803.534 KB) | DOI: 10.22146/jpki.44506

Abstract

 Background: In the Competence Standards of Indonesian Doctor (SKDI) which will ratify, interprofessional communicationand collaboration are competencies that Indonesian doctors mustown. Therefore, the implementation of interprofessional education (IPE) is a must for medical education institutions. Medical Faculty of the Universitas Pembangunan Nasional Veteran Jakarta (FK UPNVJ) has not specifically built the IPE module but has introduced aspects for interprofessional collaboration in the curriculum. Evaluation of the perceptions and readiness of students about IPE learning in students of academic year 2, 3 and 4 using the Indonesian version of The Interdisciplinary Education Perception Scale (IEPS) and Readiness for Interprofessional Learning Scale (RIPLS) showed that there were no differences in IEPS scores on student years 2nd and 3rd but decreased in 4th year students and the best RIPLS scores in the 3rd year and decreased in the 4th year. Against these results, intervention recommendations are needed to obtain results following the demands and expectations.Objectives: This study aims to obtain recommendations made based on disscussion and analysis of teaching staff of the FK UPNVJ about the results of evaluating perceptions and readiness of the FK UPNVJ at the academic stage.Methods: The research was a phenomenological qualitative research. Data collection through the opened-ended on Focus Group Discussion and in-depth interviews with teaching staff of the FK UPNVJ.Results: The teaching staff stated the importance of implementing interprofessional education by forming a curriculum. The curriculum must be more focused on interprofessional communication and collaboration since the academic stage. This implementation can be done well if get full support from the dean and the rector. The teacher staff, dean and rector’s commitment to the implementation of interprofessional education is the demand as well as the hope for the implementation of interprofessional educationConclusion: The obligation to implement interprofessional education is not natural, but it is not impossible. Several things can be done so that doctors graduating from FK UPNVJ can own communication and interprofessional collaboration competencies. 
Pelatihan dan Penyuluhan mengenai Bahaya Kanker Serviks pada Kelompok Ibu-ibu Pengajian di Pangkalan Jati, Cinere, Depok Tiwuk Susantiningsih; Feda Anisah Makkiyah; Yuni Setyaningsih
Jurnal Pengabdian Pada Masyarakat Vol 5 No 2 (2020)
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30653/002.202052.319

Abstract

TRAINING AND EDUCATION ABOUT THE DANGER OF CANCER SERVICS IN WOMEN GROUP OF PANGKALAN JATI, CINERE, DEPOK. Cervical cancer was a chronic disease due to a tumor in the cervix that was uncontrolled tissue growth and damage to the surrounding normal tissue. Citizen still does not understand the signs of disease and its dangers. Early screening of cervical cancer was done with the aim to reduce the incidence of cervical cancer. Based on an analysis of the situation and the results of a preliminary survey, the problems faced by partners are the lack of knowledge and skills of women about cervical cancer, as well as its prevention in RT 007/RW 007, Pangkalan Jati Village. This program counseling activities about the dangers of cervical cancer can be well received by the counseling participants seen with enthusiasm and questions raised. Participants gained additional knowledge about the dangers of cervical cancer, it can be seen by the presence of an increase in the value of post-test after counseling about the dangers of cervical cancer by 2%, from 84% to 86%.
PENYULUHAN DAN SURVEI TENTANG PENDAPAT MASYARAKAT TENTANG COVID SETELAH 5 BULAN DI DESA SUKAMAKMUR, KECAMATAN JONGGOL, JAWA BARAT Feda Makkiyah; Yuni Setyaningsih
INTEGRITAS : Jurnal Pengabdian Vol 4 No 2 (2020): DESEMBER
Publisher : Relawan Jurnal Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/integritas.v4i2.780

Abstract

Pandemi COVID-19 telah menimbulkan morditas dan mortalitas di Indonesia. Setelah 5 bulan penyebaran, COVID-19 belum terlihat mereda. Pengabdian ini berusaha meningkatkan aspek promotif dengan memberikan edukasi kepada masyarakat pedesaan di Desa Sukamakmur, Kabupaten Bogor, Jawa Barat. Metode pengabdian dilakukan di awal bulan Agustus 2020 dengan memberikan penyuluhan dan memberikan kuesioner untuk mengetahui tingkat pengetahuan dan perilaku masyarakat tentang kebiasaan hidup sehat. Hasil pengabdian kepada masyarakat tentang penyuluhan covid setelah 5 bulan penyebaran COVID-19 di Indonesia memberikan petunjuk bahwa pengetahuan masyarakat masih rendah tentang covid dan kebiasaan masyarakat di desa Sukamakmur tentang perilaku pencegahan COVID-19 sudah cukup baik.
PENINGKATAN PENGETAHUAN DAN KETRAMPILAN MEMBUAT WEDANG JAHE SERAI UNTUK PENGUATAN IMUNITAS MENCEGAH COVID-19 DI DESA PANGKALAN JATI CINERE DEPOK Yuni Setyaningsih; Tiwuk Susantiningsih; Agneta Irmarahayu; Fajriati Zulfa
INTEGRITAS : Jurnal Pengabdian Vol 5 No 2 (2021): DESEMBER
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat - Universitas Abdurachman Saleh Situbondo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/integritas.v5i2.977

Abstract

COVID-19 adalah penyakit menular disebabkan SARS-CoV-2 yang telah menyebar menjadi pandemi di seluruh dunia termasuk Indonesia. COVID-19 dapat berupa penyakit ringan atau sampai kematian dan obat untuk COVID-19 belum ada. Indonesia merupakan negara yang kaya akan tanaman herbal, yang sudah banyak digunakan di masyarakat. Dua tanaman herbal yang sering digunakan adalah jahe dan serai. Berdasarkan penelitian tanaman ini dapat memelihara kesehatan dan meningkatkan sistem imun. Tujuan pengabdian masyarakat ini adalah untuk memberikan pengetahuan tentang manfaat jahe serai dan keterampilan membuat wedang jahe serai dalam rangka peningkatan sistem imun pada masa pandemi COVID-19. Metode yang digunakan adalah penyuluhan dan demonstrasi pembuatan wedang jahe serai serta mengikutsertakan responden untuk berpartisipasi aktif dalam pembuatan wedang jahe serai. Pada pengabdian masyarakat ini didapatkan hasil adanya peningkatan pengetahuan responden mengenai manfaat wedang jahe serai dan cara pembuatannya. Diharapkan setelah pengabdian masyarakat ini responden dapat menerapkan dan mengaplikaskan pengetahuan yang didapatkan dalam rangka meningkatkan sistem imun pada masa pandemi COVID-19