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Nemay A. Ndaong
Laboratorium Anatomi, Fisiologi, Farmakologi dan Biokimia Fakultas Kedokteran Hewan Universitas Nusa Cendana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health Veterinary

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EFEKTIVITAS BAKTERI ASAM LAKTAT ASAL CAIRAN ISI RUMEN SAPI BALI TERHADAP BERBAGAI VARIABEL MUTU SILASE JAGUNG Frans Umbu Datta; Nadya Daramuli Kale; Annytha Ina Rohi Detha; Imanuel Benu; Nancy D. F. K. Foeh; Nemay A. Ndaong
JURNAL KAJIAN VETERINER PROSIDING SEMINAR NASIONAL KE-7
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v0i0.1587

Abstract

Silage is the result of fermentation from feed ingredients that have high water content (about 50% -80%) such as corn (Zeamays L), in a vacuum (anaerobic) by lactic acid bacteria. Making silage is one alternative to traditional biotechnology that can be done, especially utilizing the availability of local food sources. The purpose of this study is to determine the ability of lactic acid bacteria isolated from rumen fluid in Bali cattle as a starter in making corn forage silage, and evaluate the quality of corn forage silage provided by lactic acid bacteria isolated from rumen fluid in Bali cattle. The method used in this research is, making probiotics, making samples and testing the quality of silage. The results of this study are, lactic acid bacteria from the rumen contents of Balinese cattle rumen can be used as corn forage silage starter, this is indicated by the silage color in the range of yellowish green to brownish green which indicates that the silage is of good quality, silage aroma in the range of score 2 , 33-2.83 which produces a fresh sour aroma to near fresh-smelling acid, silage pH before being injected with Escherichia coli with an average of 4.51-4.81 which is within the normal range. Corn forage silage given lactic acid bacteria from the rumen contents of Bali cattle showed good quality, this is seen from the final silage results given by pathogenic bacteria Escherichia coli which showed silage color with an average of 1.83-2.58 indicating that the silage color included in the optimal range of brownish green, silage aroma in the range of 1.42-2.75 which gives a fresh sour aroma, silage pH in the range of 4.42-4.58 which indicates silage is in good range, dry silage content ranges from 32.4% -34.4% which is below the normal range, and the average percentage of damage is 0% - 3% which shows the difference in damage presentation between silage given by lactic acid bacteria and control treatment.
DESKRIPSI MORFOLOGIS NEMATODA SALURAN PENCERNAAN KAMBING KACANG (Capra hircus aegagrus) DI KOTA KUPANG-NUSA TENGGARA TIMUR Frans Umbu Datta; Theresia Tinenti; Annytha Ina Rohi Detha; Nancy D. F. K. Foeh; Nemay A. Ndaong
JURNAL KAJIAN VETERINER PROSIDING SEMINAR NASIONAL KE-7
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v0i0.1588

Abstract

Local Goats (Capra hircus aegagrus) is a type of small ruminants and is a home-grown in Indonesia which is very susceptible to attack by diseases caused by gastrointestinal parasites, especially in developing countries, causing losses to infected animals and economic losses to farmers. This study aims to know the morphology and total of eggs and adult worms, in the digestive tract in Kupang City- East Nusa Tenggara. This study uses work methods including total calculation and identification of worm eggs using the McMaster method, aiming to calculate the number of worm eggs and used to identify the morphology of worm eggs, total testing and morphology of adult worms in the digestive tract aims to determine the number of adult worms in the digestive tract, Semichenetic Acetic Camine staining aims to confirm and clarify the type from the results of identification of adult worms found in goat intestines after it is determined the dominant type of adult worms in the digestive tract of local goats. The results showed that there were three types of worm eggs found from 20 local goats, namely Trichuris sp as many as 5, Strongyloides sp as many as 63, and Strongyloid sp as many as 304 and there were three categories of infection, namely infection-free category in two goats (TTGT = 0), mild infections in three goats (TTGT = 300-400 or ranged from 1-499), and moderate infections in 15 goats (TTGT = 500-2850 or ranged from 500-> 5000). The results of the total study of adult worms in the digestive tract from four local goats found one type of digestive tract worms, namely Trichuris sp as many as 120 of the two goats (numbers 2 and 4). Trichuris sp as the most dominant type of worm in the digestive tract of local goats in Kupang City, East Nusa Tenggara.
UJI AKTIVITAS ANTIMIKROBA BAKTERI ASAM LAKTAT CAIRAN RUMEN TERHADAP PERTUMBUHAN Salmonella Enteritidis, Bacillus cereus, Escherichia coli DAN Staphylococcus aureus MENGGUNAKAN METODE DIFUSI SUMUR AGAR Frans Umbu Datta; Angela Novita Daki; Imanuel Benu; Annytha Ina Rohi Detha; Nancy D. F. K. Foeh; Nemay A. Ndaong
JURNAL KAJIAN VETERINER PROSIDING SEMINAR NASIONAL KE-7
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v0i0.1590

Abstract

Rumen liquid is a waste product of slaughterhouse that has the potential to be a pollutant, contains lactic acid bacteria which can be used as bio preservatives in food. The purpose of this study was to identify the antimicrobial activity of lactic acid bacteria (LAB) isolates from rumen fluid against Gram positive and Gram negative bacteria using well diffusion and disc diffusion methods and using lactic acid bacteria isolates (supernatant) and non-filtrate from rumen fluid. The main research materials used were LAB rumen fluid isolates, MRSA media (Mann Rogosa Sharpe Agar), MRSB media (Mann Rogosa Sharpe Broth), MHA media (Muller Hinton Agar), and pathogenic bacteria Bacillus cereus, Staphylococcus aureus, Escherichia coli and Salmonella Enteritidis. The results of the study showed that the LAB of rumen fluid carried out as an active LAB with Gram positive characteristics, round shape, negative catalase and non motile. Based on the results of testing the antimicrobial activity of lactic acid bacteria from rumen fluid isolates against pathogenic Gram positive (B. cereus and S. aureus) and Gram negative bacteria (Escherichia coli and Salmonella Enteritidis) using well and disc diffusion methods showed that Gram negative bacteria were more sensitive to antimicrobial of LAB compared to Gram positive bacteria. The diameter of the larger inhibition zone is produced using the disc method with the inhibition zone diameter range of 13.66-28.3 mm, while the well method ranges from 0-24.2 mm. The antimicrobial activity of LAB using non filtrate BAL produce inhibition zone diameter size range of 0-26.1 mm, while the filtrate BAL produce inhibition zone diameter range of 0-28.3 mm with the optimum time to produce antimicrobial activity 48 hours compared to 24 hours after incubation.
PERSEBARAN SEL HASIL VAGINA SMEAR KAMBING KACANG LOKAL (CAPRA AEGAGRUS) DI KOTA KUPANG Nancy Foeh; Frans Umbu Datta; Annytha Ina Rohi Detha; Nemay A. Ndaong; Maria Moi
JURNAL KAJIAN VETERINER Vol 7 No 2 (2019): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v7i2.1985

Abstract

Penelitian ini bertujuan untuk mengetahui gambaran sel vagina kambing kacang lokal (capra aegagrus) selama satu siklus estrus. Sampel diambil dari 10 ekor kambing kacang betina produktif dengan kisaran umur 12-18 bulan . Sampel di buat preparat ulas dengan motode roll, dan diwarnai dengan Giemsa ,dilakukan dengan jarak interval 24 jam diulang selama 20 hari. Hasil menunjukkan persebaran sel-sel kornifikasi, superfisial, intermediet ,sel parabasal dan leukosit selama 20 hari pengamatan. Kesimpulannya, panjang siklus estrus kambing kacang lokal (capra aegagrus) 17-20 hari dengan panjang tahapan proestrus 1-2 hari, estrus 1-2 hari, metestrus 3-5 hari dan diestrus 12-15 hari.
Co-Authors Angela Novita Daki Annytha Detha Imanuel Benu Maria Moi Nadya Daramuli Kale Nancy D. F. K. Foeh Theresia Tinenti