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All Journal AGRIVITA, Journal of Agricultural Science AGRINULA: Jurnal Agroteknologi dan Perkebunan
Octanina Sari Sijabat
Program Studi Budidaya Perkebunan, Fakultas Pertanian Dan Peternakan, Universitas Tjut Nyak Dhien, Medan 20123, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Exploration of Symbiotic Bacteria of Oryctes rhinoceros (Coleoptera: Scarabaeidae) Larvae from Oil Palm Empty Fruit Bunches Marheni Marheni; Edhi Martono; Octanina Sari Sijabat
AGRIVITA, Journal of Agricultural Science Vol 43, No 1 (2021)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v43i1.2301

Abstract

Oil palm empty fruit bunches (OPEFB) were utilized as the source of organic fertilizer, but become a medium of Oryctes rhinoceros proliferation in oil palm plantations. O. rhinoceros uses cellulose, hemicellulose and lignin in the OPEFB as energy sources for larvae growth and development. In the process of breaking and combining complex compounds into simple molecules, they are establishing symbiotic relationship with bacteria residing in the digestive tract of O. rhinoceros larvae. Isolation of the symbiotic bacteria of the O. rhinoceros larvae was carried out with the aim of identifying bacteria in the digestive tract of the third instar found in the OPEFB. This research was done by using a descriptive method. Exploration of symbiotic bacteria of O. rhinoceros larvae was conducted through two stages. The first step was attained by isolating, growing and multiplying the bacteria and conducting biochemical testing. The second phase was accomplished by molecular testing for bacterial identification. The results showed that bacterial isolates were found and and after molecularly sequenced based on 6S rDNA, the species were identified as Bacillus stratosphericus, B. siamensis, B. cereus, Haemophilus parainfluenzae, Achromobacter xylosoxidans, and Alcaligenes faecalis. The research also found several species of bacteria originating from the hindgut larvae O. rhinoceros.
Aplikasi Pupuk Anorganik dan Waktu Rendam Benih dengan Bakteri Legum pada Kacang Tanah di Lahan Pasang Surut Billy Andreas Putra; Tengku Boumedine Hamid Zulkifli; Yunida Berliana; Octanina Sari Sijabat
Agrinula : Jurnal Agroteknologi dan Perkebunan Vol 3 No 2 (2020): Agrinula : Jurnal Agroteknologi dan Perkebunan
Publisher : Prodi. Agroteknologi dan Perkebunan, Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/agri.v3i2.103

Abstract

Introduction: The purpose of this study was to determine the dosage of inorganic fertilizers, soaking time of the inoculation of the legume bacteria strains which were suitable for the growth and yield of peanut in tidal lowland. Materials and Methods: This research was conducted at tidal lowland in Sei Ular Village, Secanggang sub-District, Langkat District, North Sumatra from September to December 2019. The research used randomized block design in factorial. The first factor is the dose of inorganic fertilizers consisting of P0 = un-fertilizer (control), P1= urea 50 kg.ha-1 + TSP 180 kg.ha-1 + KCl 50 kg.ha-1, P2= urea 75 kg.ha-1 + TSP 205 kg.ha-1 + KCl 75 kg.ha-1, P3= urea 100 kg.ha-1 + TSP 230 kg.ha-1 + KCl 100 kg.ha-1. The second factor was the soaking time of the Bradyrhizobium japonicum bacterial strain consisting of T0= un-soaking time, T1= soaking time for 3 minutes, T2= soaking time for 6 minutes, and T3= soaking time for 9 minutes. Parameters were analyzed using ANOVA and BNJ analysis 5%.ยท Results: The application of urea 50 kg.ha-1 + TSP 180 kg.ha-1 + KCl 50 kg.ha-1 significantly increased the plant height, number of leaves, and root volume and showed the highest number of active nodules, dry pods.plot-1 and seed weight.plot-1 compared to other doses. The seed soaking time with the Bradyrhizobium japonicum strain and their interactions showed insignificant differences in all parameters of peanut plants in tidal lowland.
Co-Authors Billy Andreas Putra Edhi Martono marheni marheni Tengku Boumedine Hamid Zulkifli Yunida Berliana