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Induction of In Vitro Culture of Potato Microtuber by Using Alar and Dark Photoperiod Application Dwiati, Murni; Anggorowati, Sulastri
AGRIVITA, Journal of Agricultural Science Vol 33, No 1 (2011)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Providing virus free potato seeds in order to increase potato production in Indonesia could be carried out by using microtuber resulting from microcutting. The growth of single node microcutting potato can initiate microtuber formation when growth inhibiting substances such as alar in combination with dark photoperiod treatment is applied. This study was aimed to elucidate the effect of alar and dark photoperiod on the date of microtuber emergence and production. The experiment was arranged in a factorial Randomized Completely Block Design in which alar concentrations i.e. 0, 10-3, 5 x 10-3, and 10-2 mg/L, served as factor I, and dark photoperiods i.e. 16, 20, and 24 hrs/day, were used as factor II. Each treatment combination was replicated three times giving rise to 36 experimental units. Data were analyzed using ANOVA (F test) followed by Duncan Multiple Range Test (DMRT) when significant effect of the treatments existed. The results showed that alar and dark photoperiod affected individually on the date of microtuber emergence, while the best alar concentration to increase microtuber production was 10-3 mg/L with 10.67 microtubers/cutting. Dark period has no significant effect on the induction of potato microtuber. Keywords: potato microtuber, alar, dark photoperiod

Induction of In Vitro Culture of Potato Microtuber by Using Alar and Dark Photoperiod Application Murni Dwiati; Sulastri Anggorowati
AGRIVITA, Journal of Agricultural Science Vol 33, No 1 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i1.38

Providing virus free potato seeds in order to increase potato production in Indonesia could be carried out by using microtuber resulting from microcutting. The growth of single node microcutting potato can initiate microtuber formation when growth inhibiting substances such as alar in combination with dark photoperiod treatment is applied. This study was aimed to elucidate the effect of alar and dark photoperiod on the date of microtuber emergence and production. The experiment was arranged in a factorial Randomized Completely Block Design in which alar concentrations i.e. 0, 10-3, 5 x 10-3, and 10-2 mg/L, served as factor I, and dark photoperiods i.e. 16, 20, and 24 hrs/day, were used as factor II. Each treatment combination was replicated three times giving rise to 36 experimental units. Data were analyzed using ANOVA (F test) followed by Duncan Multiple Range Test (DMRT) when significant effect of the treatments existed. The results showed that alar and dark photoperiod affected individually on the date of microtuber emergence, while the best alar concentration to increase microtuber production was 10-3 mg/L with 10.67 microtubers/cutting. Dark period has no significant effect on the induction of potato microtuber. Keywords: potato microtuber, alar, dark photoperiod

Penggunaan Marka RAPD Sebagai Penduga untuk Membedakan Jenis Kelamin pada Kantung Semar Nepenthes Gymnamphora Koleksi Kebun Raya Baturraden Dini Rizki Pertiwi; Murni Dwiati; Agus Hery Susanto
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Nepenthes gymnamphora is an endemic pitcher plant species in Java Island and one of the plant collections of Baturraden Botanical Garden. N. gymnamphora is a dioecious plant and its sex cannot be differed in vegetative development. Conservation efforts can be carried out more efficiently when sex identification is performed earlier thus leading to effective development of particular sexes. Random Amplified Polymorphic DNA (RAPD) technique is one molecular approach that can be employed in early identification of N. gymnamphora sex. The aims of this study are to assess whether there is difference of RAPD patterns between male and female N. gymnamphora and to find out how the difference is. Explorative method was applied to this study involving five N. gymnamphora individuals of Baturaden Botanical Garden collection of different sexes as samples. Genomic DNAs were extracted from youngest leaves of the five samples (two males, two females and one individual of unknown sex) and then used as templates to amplify RAPD markers. Five random primers were used in the amplification, i.e. OPK-16, OPP-15, OPA-15, OPP-08, and OPD-20. Two primers, i.e. OPP-08 (5’-ACATCGCCA-3’) and OPD-20 (5’-ACTTCGGCCAC-3’), produced RAPD bands of approximately 300 bp in males and sexually unknown individual. These bands did not appear in females, so that it can be presumably related to sex determination genes in N. gymnamphora. Primer OPP-08 also produced RAPD bands of approximately 250 bp in females individual. These bands did not appear in males, so it can be presumably related to sex determination genes in N. gymnamphora.

Aplikasi Paklobutrazol dan KNO3 untuk Meningkatkan Kualitas dan Kuantitas Bunga Potong Anggrek Dendrobium ‘Sarifah Fatimah’ Murni Dwiati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 1 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.1.150

A study on the effect of interactions between paclobutrazole and KNO3 application on the quality and quantity of Dendrobium ‘Sarifah Fatimah’ orchid cutflower was conducted. The experiment was arranged in a Randomized Complete Block Design (RCBD) employing two factors, i.e. paclobutrazole doses (100, 150, 200 ppm) and KNO3 doses (100, 200, and 300 ppm), replicated three times respectively providing 27 experimental units. The results showed that paclobutrazole and KNO3 application had significant effect on the reproductive parameters. To accelarate flowering and higher quality and quantity of the flowers, paclobutrazole of 100 ppm were applied at 2 and 5 weeks after adaptation combined with KNO3 of 100 ppm applied twice at interval of 3 weeks after paclobutrazole application could be given.

Kandungan Bahan Aktif Fomesafen dalam Biji Kedelai dan Kacang Hijau dari Tanaman yang Ditumbuhkan pada Tanah Bekas Paparan Reflex Murni Dwiati; Iman Budisantoso
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 1 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.1.78

A research has been conducted to understand the responses of soybean and mungbean to the persistence of Reflex residuals in soils referring to its active substance contents, i.e. fomesafen, in the plants grown in Reflex-exposed soils. The study was carried out experimentally in glass house from June to November 2003 employing Completely Randomized Design (CRD) with treatments arranged factorial. Two factors were used, the first of which was plant species (soybean and mungbean) while the second was Reflex concentrations (0, 25, 50, 100, and 200 ppm). Each treatment combination was replicated three times. The parameters examined comprised of fomesafen contents in the seeds, number of seeds per pod, weight of 100 seeds, and weight of total seeds. To measure fomesafen contents in the seeds, Gas Liquid Chromatograph with ECD detector and column containing 10% OV-101 chromosorb WHF 80/100 mesh of 2m lengths and 3 mm diameter was applied. Fomesafen residuals in the soils of 5 month persistence had no harmful effect on both soybean and mungbean plants. As well, they had no significant effect on the production variables of both plants. Soybean seeds planted in the 5-month Reflex-exposed soils still contained fomesafen while the corresponding mungbean seeds did not.

Peran Paklobutrazol dan Pupuk dalam Mengendalikan Tinggi Tanaman dan Kualitas Bunga Krisan Pot Rochmatino Rochmatino; Iman Budi Santoso; Murni Dwiati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 2 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.2.196

The objective of this study was to determine the effect of paklopbutrazol concentration and fertilizer on plant height and flower quality of potted chrysanthemum. The experimental method was used with factorial design. The first factor was paklobutrazol with the concentration as follow: 0, 100, 200, and 300 ppm. The second factors were cow, chicken, and goat manure. Parameters observed were plant height, time of flowers emerging, flower diameter and freshness. The results showed that certain concentration of paklobutrazol might inhibited plant height, time of flowers emerging, and shortening of flowers freshness, while chicken manure fertilizer spurred flower diameter.

Induksi Kalus Nepenthes mirabilis(Lour.) Druce Menggunakan NAA danTDZ Nina Nurussakinah; Murni Dwiati; Iman Budisantoso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4242

Nepenthes sp. has a characteristic in the form of a modified leaf tip into a pitcher. Nepenthes mirabilis (Lour.) Druce in its original habitat is widely hunted for sale. Propagation of N. mirabilis in nature has very little success rate, to overcome this it is necessary to propagate it by means of in vitro culture and use young leaf explants to form callus. Growth regulators such as Auxins (NAA) and Cytokinins (TDZ) can stimulate callus growth. Auxins can stimulate callus formation by stimulating cell expansion and cytokinins can stimulate the cell division process. The purpose of this study was to determine the effect of NAA and TDZ in inducing N. mirabilis callus and to determine the interaction between NAA and TDZ in inducing N. mirabilis callus. The experimental design used was a factorial CRD pattern, with two factors, namely NAA and TDZ. NAA consists of four levels, namely, 0 mg / L (N0); 0.5 mg / L (N1), 1 mg / L (N2) and 1.5 mg / L (N3). TDZ also consists of four levels, namely 0 mg / L (T0); 1 mg / L (T1); 2 mg / L (T2) and 3 mg / L (T3). Each treatment was repeated three times. The results showed that the addition of NAA and TDZ had an effect on callus induction of N. mirabilis. The TDZ concentration of 1 mg / L (T1) stimulated callus induction, especially the percentage of callus formation and callus diameter. Meanwhile, NAA 1 mg / L (N2) boosted the percentage of N. mirabilis callus formation.

APLIKASI NAA PADA PROPAGASI KANTONG SEMAR Nepenthes adrianii Batoro, Wartono & Jebb Rosa Amalia Wahdani; Murni Dwiati; Kamsinah Kamsinah
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 3 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.5481

Nepenthes adrianii is an endemic species of Mount Slamet. The existence of this species has begun to be threatened. This situation encourages efforts to conserve N. adrianii, which is through propagation using stem cuttings of N. adrianii. In order for the propagation of stem cuttings to grow properly, it is necessary to add growth hormone (ZPT) from the auxin group such as NAA (Napthalene Acetic Acid). This research has been conducted to study the effect of NAA and its effective consentration for the growth of N. adrianiistem cuttings. This research was conducted using experimental methods. The experimental design used was a Randomized Completely Block Design (RCBD). The block in this study is total leaves per cuttings. Block 1 is the leafless cuttings, block 2 is cuttings with one leaf, block 3 is cuttings with two leaves, and block 4 is cuttings with three leaves. The NAA concentrations in this study are 0, 5, 10, 15, 20, and 25 ppm. There are a total of 24 experimental units. The variable observed was the growth of N. adrianii stem cuttings. Parameters observed in this study are days taken for sprouting, number of shoots, number of leaves, leaf length, and leaf width. The results were analyzed using Variety Analysis and then analyzed with Tukey's test at the test level of 0.05 at the time of significant treatment. The results showed that the application of NAA did not affect the growth of the stem cuttings of N. adrianii. The best concentration of NAA could not be determined because there was no effect of NAA on the growth of N. adrianii stem cuttings.

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