Paul Kristiansen
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SOME WEED SPECIES AFFECTING SOYBEAN NODULATION AND NODULE FUNCTION Chaniago, Irawati; Taji, Acram; Kristiansen, Paul; Jessop, Robin
AGRIVITA, Journal of Agricultural Science Vol 34, No 2 (2012)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Experiments aimed at examining the effect of aqueous extracts of three weed species on nodulation and nodule function of soybean cv. Melrose have been carried out at the Laboratory of Plant Physiology and Biotechnology, Department of Agronomy and Soil Science, University of New England, Australia. Aqueous extracts of fresh weed material (Amaranthus powellii, Cyperus rotundus and Paspalum dilatatum) at the concentration of 10% (w/v) were added to a minus-nitrogen Hoagland’s nutrient solution in which the soybean plants were grown with 14 hours day length, day and night temperatures of 28 and 20°C, respectively, light intensity of 790 mmol/m2/s, and the relative humidity of 65%. The plants were kept for three weeks prior to the measurement of activity of nitrogenase enzyme and ammonium content of the root nodules. Results demonstrate that all weed extracts reduced the nodulation and nitrogenase activities (ARA) of soybean cv. Melrose. Amaranth was most inhibitory to nitrogenase enzyme activity. However, amaranth extract was less inhibitory than nutgrass extract in total ammonium content.
SOME WEED SPECIES AFFECTING SOYBEAN NODULATION AND NODULE FUNCTION Chaniago, Irawati; Taji, Acram; Kristiansen, Paul; Jessop, Robin
AGRIVITA, Journal of Agricultural Science Vol 34, No 2 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i2.118

Abstract

Experiments aimed at examining the effect of aqueous extracts of three weed species on nodulation and nodule function of soybean cv. Melrose have been carried out at the Laboratory of Plant Physiology and Biotechnology, Department of Agronomy and Soil Science, University of New England, Australia. Aqueous extracts of fresh weed material (Amaranthus powellii, Cyperus rotundus and Paspalum dilatatum) at the concentration of 10% (w/v) were added to a minus-nitrogen Hoagland’s nutrient solution in which the soybean plants were grown with 14 hours day length, day and night temperatures of 28 and 20°C, respectively, light intensity of 790 mmol/m2/s, and the relative humidity of 65%. The plants were kept for three weeks prior to the measurement of activity of nitrogenase enzyme and ammonium content of the root nodules. Results demonstrate that all weed extracts reduced the nodulation and nitrogenase activities (ARA) of soybean cv. Melrose. Amaranth was most inhibitory to nitrogenase enzyme activity. However, amaranth extract was less inhibitory than nutgrass extract in total ammonium content.
EFFECT OF TWO SEAWEED PRODUCTS ON RADISH (RAPHANUS SATIVUS) GROWTH UNDER GREENHOUSE CONDITIONS Ramal Yusuf; Paul Kristiansen; Nigel Warwick
AGROLAND: The Agricultural Sciences Journal Vol 2, No 1 (2015)
Publisher : Tadulako University

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Abstract

Several studies have revealed a wide range of beneficial effects of seaweed products applications on plants growth and development. Seaweeds may cause different effect in plant responses because there are a range possible factors acting in concert. These factors may include to the amount of plant growth regulator (PGR) and nutrients present in the products. PGR activity in two seaweed products (Maxicrop® and Seasol®) was investigated using Radish (Raphanus sativus). Treatments included a control, standard concentrations of the seaweed Products (SS1 and MC1), 2 and 4 times the recommended rates (SS2, SS4, MC2 and MC4) ashed seaweed products (SS-ash and MC-ash), and equivalent amounts of N, P and K to that found in seaweed products (SS-NPK and MC-NPK). The results obtained show that both Maxicrop® and Seasol® can significantly increase crop performance. Maxicrop® increased chlorophyll content and root and shoot growth, while Seasol® only increased the chlorophyll content
EFFECT OF TWO SEAWEED PRODUCTS ON RADISH (RAPHANUS SATIVUS) GROWTH UNDER GREENHOUSE CONDITIONS Ramal Yusuf; Paul Kristiansen; Nigel Warwick
AGROLAND: The Agricultural Sciences Journal Vol 2, No 1 (2015)
Publisher : Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Several studies have revealed a wide range of beneficial effects of seaweed products applications on plants growth and development. Seaweeds may cause different effect in plant responses because there are a range possible factors acting in concert. These factors may include to the amount of plant growth regulator (PGR) and nutrients present in the products. PGR activity in two seaweed products (Maxicrop® and Seasol®) was investigated using Radish (Raphanus sativus). Treatments included a control, standard concentrations of the seaweed Products (SS1 and MC1),    2 and 4 times the recommended rates (SS2, SS4, MC2 and MC4) ashed seaweed products (SS-ash and MC-ash), and equivalent amounts of N, P and K to that found in seaweed products (SS-NPK and MC-NPK). The results obtained show that both Maxicrop® and Seasol® can significantly increase crop performance. Maxicrop® increased chlorophyll content and root and shoot growth, while Seasol® only increased the chlorophyll content.   Key Words: Plant growth regulator, radish, seaweed.
BIOASSAY DEVELOPMENT USING PEA (Pisum sativum L.) Ramal Yusuf; Paul Kristiansen; Nigel Warwick
AGROLAND: The Agricultural Sciences Journal Vol 1, No 1 (2014)
Publisher : Tadulako University

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Abstract

The term bioassay is used to describe the use of living material to test the effect of known and putative biologically active substances. In places where advanced analytical equipment is not available, rapid screening using simple, affordable methods is needed. Seeds of Seeds of peas were germinated (Arditti and Dunn, 1969) and grown in the dark at 20°C for 2 days. Five pre-germinated seeds immersed in 30 mL solution containing test solutions of auxins (IBA), cytokinins (kinetin) and a gibberellin (GA3) all seeds were distributed at randomly to Petri dishes containing the test solution. Auxin activity peak was equivalent to 0.005 µM, while cytokinin activity peaked at 0.5 µM, and gibberellin at 0.5 to 5 µM based on root measurement.   KeyWords : Bioassay, elongation, Pea.
Bioassay Development Using Pea (Pisum sativum L.) Ramal Yusuf; Paul Kristiansen; Nigel Warwick
AGROLAND The Agricultural Sciences Journal (e-Journal) Vol 1 No 1 (2014): June
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/agroland.v1i1.288

Abstract

The term bioassay is used to describe the use of living material to test the effect of known and putative biologically active substances. In places where advanced analytical equipment is not available, rapid screening using simple, affordable methods is needed. Seeds of Seeds of peas were germinated (Arditti and Dunn, 1969) and grown in the dark at 20°C for 2 days. Five pre-germinated seeds immersed in 30 mL solution containing test solutions of auxins (IBA), cytokinins (kinetin) and a gibberellin (GA3) all seeds were distributed at randomly to Petri dishes containing the test solution. Auxin activity peak was equivalent to 0.005 µM, while cytokinin activity peaked at 0.5 µM, and gibberellin at 0.5 to 5 µM based on root measurement.
Effect of Two Seaweed Products on Radish (Raphanus sativus) Growth Under Greenhouse Conditions Ramal Yusuf; Paul Kristiansen; Nigel Warwick
AGROLAND The Agricultural Sciences Journal (e-Journal) Vol 2 No 1 (2015): June
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/agroland.v2i1.333

Abstract

Several studies have revealed a wide range of beneficial effects of seaweed products applications on plants growth and development. Seaweeds may cause different effect in plant responses because there are a range possible factors acting in concert. These factors may include to the amount of plant growth regulator (PGR) and nutrients present in the products. PGR activity in two seaweed products (Maxicrop® and Seasol®) was investigated using Radish (Raphanus sativus). Treatments included a control, standard concentrations of the seaweed Products (SS1 and MC1), 2 and 4 times the recommended rates (SS2, SS4, MC2 and MC4) ashed seaweed products (SS-ash and MC-ash), and equivalent amounts of N, P and K to that found in seaweed products (SS-NPK and MC-NPK). The results obtained show that both Maxicrop® and Seasol® can significantly increase crop performance. Maxicrop® increased chlorophyll content and root and shoot growth, while Seasol® only increased the chlorophyll content.