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All Journal AGRIVITA, Journal of Agricultural Science Akta Agrosia Biofarm : Jurnal Ilmiah Pertanian
Eko Suprijono
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Agriculture, Biological Sciences & Forestry

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IDENTIFICATION OF M4 GAMMA IRRADIATED MAIZE MUTANT BASED ON RAPD MARKERS Rustikawati, Rustikawati; Suprijono, Eko; Romeida, Atra; Herison, Catur; Sutjahjo, Surjono H.
AGRIVITA, Journal of Agricultural Science Vol 34, No 2 (2012)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Gamma irradiation to induce mutation in plant has been used intensively since several decades ago.  On maize, 275 Gy gamma irradiation have been known to increase genetic variability indicated by their morphological variation.  Identification on genetic changes by molecular technique is important to answer whether there is mutation happening on DNA level of the plants.   The objective of this research was to identify RAPD marker polymorphism on gamma irradiation mutants compared to their parents.  The initial step of the research was to select random primers could positively amplify the maize DNA.  The result showed that selection on 60 random primers yielded 15 primers that positively amplified the maize DNA.  Amplification on both mutants and their parents by those 15 selected primers indicated that only 5 primers yielding polymorphism between mutants and their parents.  Polymorphisms on mutant G1, G3 and G6 were detected on one locus, meanwhile on mutant G7, G8 and G9 were on two loci.
ERTUMBUHAN DAN HASIL TANAMAN KACANG BOGOR PADA BERBAGAI TINGKAT KERAPATAN TANAMDAN FREKUENSI PENYIANGAN Edhi Turmudi; Eko Suprijono
Biofarm Jurnal Ilmiah Pertanian Vol 13, No 8 (2010): BIOFARM JURNAL ILMIAH PERTANIAN
Publisher : Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31941/biofarm.v13i8.271

Abstract

Upaya pemehunan konsumsi kacang bogor melalui peningkatan produksi dihadapkan pada permasalahan  gangguan gulma yang dapat diatasi dengan pengaturan kerapatan tanaman dan penyiangan. Penelitian bertujuan untuk mendapatkan frekuensi penyiangan pada setiap kerapatan tanaman kacang bogor yang pertumbuhan dan hasilnya tertinggi. Pelaksanaannya dengan menguji dua macam perlakuan yaitu tingkat kerapatan tanaman yang terdiri atas tiga taraf dan frekuensi penyiangan terdiri atas empat taraf disusun secara faktorial dengan Rancangan Acak Kelompok Lengkap (RAKL) yang  dilakukan dalam bentuk percobaan lapangan. Penyiangan dua kali secara nyata meningkatkan pertumbuhan dan hasil kacang bogor. Hasil biji kering per petak tertinggi sebesar 1559,37 g lebih dari tanpa penyiangan. Indeks luas daun, jumlah daun, biomassa tanaman, jumlah polong muda, dan jumlah polong pertanaman tertinggi pada kerapatan tanaman 150.000 per hektar.Keta kunci : Kacang bogor, penyiangan
ERTUMBUHAN DAN HASIL TANAMAN KACANG BOGOR PADA BERBAGAI TINGKAT KERAPATAN TANAMDAN FREKUENSI PENYIANGAN Edhi Turmudi; Eko Suprijono
Biofarm Jurnal Ilmiah Pertanian Vol 13, No 8 (2010): BIOFARM JURNAL ILMIAH PERTANIAN
Publisher : Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31941/biofarm.v13i8.272

Abstract

Upaya pemehunan konsumsi kacang bogor melalui peningkatan produksi dihadapkan pada permasalahan  gangguan gulma yang dapat diatasi dengan pengaturan kerapatan tanaman dan penyiangan. Penelitian bertujuan untuk mendapatkan frekuensi penyiangan pada setiap kerapatan tanaman kacang bogor yang pertumbuhan dan hasilnya tertinggi. Pelaksanaannya dengan menguji dua macam perlakuan yaitu tingkat kerapatan tanaman yang terdiri atas tiga taraf dan frekuensi penyiangan terdiri atas empat taraf disusun secara faktorial dengan Rancangan Acak Kelompok Lengkap (RAKL) yang  dilakukan dalam bentuk percobaan lapangan. Penyiangan dua kali secara nyata meningkatkan pertumbuhan dan hasil kacang bogor. Hasil biji kering per petak tertinggi sebesar 1559,37 g lebih dari tanpa penyiangan. Indeks luas daun, jumlah daun, biomassa tanaman, jumlah polong muda, dan jumlah polong pertanaman tertinggi pada kerapatan tanaman 150.000 per hektar.Keta kunci : Kacang bogor, penyiangan
Pengujian Berbagai Tipe Tanam Jajar Legowo terhadap Hasil Padi Sawah Dia Novita Sari; Sumardi Sumardi; Eko Suprijono
Akta Agrosia Vol 17, No 2 (2014)
Publisher : Faculty of Agriculture, The University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.29 KB) | DOI: 10.31186/aa.17.2.115-124

Abstract

Planting wetland paddy by setting row space could be a method to increase rice production. Legowo system is row planting method where we are able to apply different row types on paddy field. The objective ofthis research was to identify the best row planting type of ‘Jajar Legowo’ on growth and yield of wetland paddy. Six row plantings, including 2:1 type (means 2 rows are splited by wide space/ legowo), 3:1 type, 4:1 type, 5:1type, 6:1 type, and 7:1 typ, were tested in a Randomized Complete Block Design with 3 replications Results of the experiment revealed that the highest growth and yield as showed by total number of tillers (28), number ofproductive tillers (25), dry-mill rice (66.16 g per plant), and yield difference of dry-mill rice (50.43% per plot) was found at row planting type 2:1. Therefore, row planting type 2:1 can be selected as the best row.
Potensi Produksi Enam Hibrida Jagung pada Ultisol di Provinsi Bengkulu Eko Suprijono; Rustikawati Rustikawati; Atra Romeida; Meko Gustian
Akta Agrosia Vol 18, No 2 (2015)
Publisher : Faculty of Agriculture, The University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4289.503 KB) | DOI: 10.31186/aa.18.2.32-39

Abstract

Ultisol is one of marginal land type quite extensively presence in Indonesia, including the province of Bengkulu. The use of varieties tolerance to acidity stress is the best manner to utilize of this type of land with relatively low cost and environmentally friendly. The objective of this research was to evaluate theyield potential of six maize hybrids developed to soil acidity tolerance. The experiment was conducted at the Experimental Farm Medan Baru, Kandang Limun Village, Muara Bangkahulu District, Bengkulu City, from August to November 2015. The experiment was arranged in a randomized completely block design with four replications. As the treatment were newly developed maize hybrids, namely CT5, CT8, CT9, CT13, CT14 and CT15. The variables measured were cob length, cob diameter, 100-seed weight and the estimated yield/ha. Qualitative variables supporting to corn yield were the cob position, seed color, seed type and seed row arrangement. The results indicated that the hybrid CT8 showed the highest estimated yield in Ultisol among hybrids evaluated, with the estimation of 6.5 tons per hectare. Qualitatively, theseeds of hybrid CT8 were a horse tooth type, pale yellow color, and stright line arrangement.
Co-Authors Atra Romeida Catur Herison Dia Novita Sari EDHI TURMUDI Meko Gustian Rustikawati Rustikawati Rustikawati Rustikawati Sumardi Sumardi Surjono H. Sutjahjo