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Catechins Green Tea Clones GMB4 Inhibit Inflammation Process of Atherosclerosis Through Decreasing TNF-Î‘ Levels Susanti, Erna; Suratno, Febiyanti
Journal of Islamic Pharmacy Vol 1, No 1 (2016): J. Islamic Pharm.
Publisher : Universitas Islam Negeri Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jip.v1i1.3458

Catechins Green tea clones GMB4 are potential natural subtances to inhibitÂ inflammation process in atherosclerosis. The purpose of this research to determine activity ofÂ Catechins Green tea clones GMB4 to decrease TNF-Î± levels, as well as to determine optimal dose of Catechins. This research was conducted at the Laboratory of Physiology of the University of Brawijaya. The method used in this research is completely randomized design with five treatments: (1) Mice with standard feed (2) mice with high-fat diet (3) mice with high-fat diet + catechins 3mg / day (4) mice with a diet high fat + catechin 6mg / day (5) mice with high-fat diet + catechin 24mg / day. The treatment was done for 60 days and measured levels of TNF-Î± at the end of the research. The results showed that Catechins green tea clones GMB4 can inhibit the inflammatory process in atherosclerosis through decreasing levels of TNF-Î± at doses of 3mg / day. The conclusion of this research is catechins at a dose of 3 mg / day can inhibit inflammation in atherosclerosis significantly.Â Keywords : GMB 4 green tea clones, Catechins, inflammation, TNF-Î±

Al-Quran dan Sains?(Bukti Kebenaran dalam Kasus Propolis Madu) Erna Susanti, MF. Zenrif dan
Khazanah: Jurnal Studi Islam dan Humaniora Vol 12, No 2 (2014)
Publisher : UIN Antasari

Exposure free radicals in the activity of this life can not be avoided, included exposure free radicals from food additive espesifically Rhodamine B. Carcinogenic effect of Rhodamin B can be caused by Poly Aromatic Hydrocarbons structure that are destructive to the liver as metabolism organ. The purpose of this study was to prove the antioxidant activity of Honey Propolis against liver damage caused by exposure of Rhodamine B as free radical sources. Indicator of liver damage can be indicated by MDA, a marker of oxidative stress that occurs in these organs. The methode was used experimental methode, with seven treatment groups, namely: (1) normal group, (2) the induction of Rhodamin B without propolis therapy group, (3) the induction of Rhodamin B with Honey Propolis therapy with three dose levels are 350 mg, 700 mg and 1050 mg that are given at intervals of two hours after the administration of Rhodamine B (4) Honey Propolis therapy groups are given at interval of 1 week aftertheadministrationof RhodamineBwiththreedoselevels.MDAlevelsweremeasuredbyTBARS method. The results showed that the levels of Malondialdehyde declined steadily in the group treated with honey propolis to less than control groups. There are significant differences in MDA levels between the treatment groups Honey Propolis therapy (p = 0.034, p <0.05). Based on these results, it can be concluded that Honey propolis dose 700 mg act as therapeutic agent in liver damage due to exposure of Rhodamine B significantly.The results ofthis studyprove thetruth ofthe Quraninthe QS. Al-Nahl (16): 69of thewonders ofhoneyin preventingliver damagedue toexposure tohazardous substancesin food.

EFEK KEFIR TERHADAP GAMBARAN HISTOLOGIS DAN KADAR MALONDIALDEHIDE HEPAR MENCIT PUTIH (MUS MUSCULUS) JANTAN GALUR BALB C DENGAN PAPARAN FORMALIN Ni Made Dwi Aryantini, Erna Susanti
Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Vol 2, No 1 (2012): Januari
Publisher : University of Muhammadiyah Malang

Kefir is a probiotic drink act as therapeutic agents in liver damage caused by exposure of formaldehyde. Therapeutic activities were estimate based on the content of kefir like antioxidants, lactic acid bacteria with yeasts, and essential amino acids. The purpose of this study was to prove the influence of kefir on the histological and levels of malondialdehyde (MDA) in liver of white mice (Mus musculus) male Balb C strain that exposure by formalin. The method used is experimental method, with three treatment groups, namely: (1) normal group, (2) the induction of formaline without kefir therapy group, (3) the induction of formaline with kefir therapy group. Histological of liver viewed by HE staining and MDA levels were measured by TBARS method. There are differences in the average number of significant cell damage induced by formalin group (p = 0030, p < 0.05). There are significant differences in MDA levels induced by formalin group (p = 0023, p < 0.05). These results are based on the ability of vitamin C, E and carotene (antioxidant) against Reactive Oxygen Species (ROS) due to exposure of formaldehyde, thus preventing the MDA levels increasing. Lactic acid bacteria and yeasts can preclude the possibility ofÂ healthy cells to be attacked by ROS with increasing the bodyâ€™s immunity. Essential amino acids can repairing damaged cells by stimulating cell regeneration. It can be concluded that kefir with a dose of 107Â CFU / ml act as therapeutic agents in liver damage due to exposure to formaldehyde.

Efek Ateroprotektif dan Vasoprotektif Katekin Teh Hijau terhadap Ekspresi eNOS pada Tikus Wistar Jantan dengan Diet Tinggi Lemak Retty Ratnawati, Erna Susanti
Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Vol 2, No 1 (2012): Januari
Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/far.v2i1.1155

Catechins , isolate from green teaÂ GMB4 clones could be developed as a potential preventive agent for atherosclerosis. This activity is thought to be based on the structure epimerisation, hydroxy phenolicÂ and galloil group of these isolates. The purpose of this study is to prove the influence catechins ,isolate from green tea, on the inhibition of the decrease eNOS expression in male wistar rats induced byÂ highÂ fat diet. The method used in this study consisted of ELISA for measurement of eNOS expression. The results showed thatÂ catechins inhibits the decreasing expression of eNOS at a dose of 6 mg / day and 24 mg / day was significantly but not dose 3mg / day. This suggests a dose 6mg / day and 24 mg / day is able to repair the damage signaling insulin on PI3K pathway caused by insulin resistance. These results are based on the ability of catechins as antioxidants. Catechins with hydroxy phenolic groupÂ is a potential electron donor to bindÂ free radicals. Polyphenol catechins may also modulateÂ signaling cellular processes such as NFkÃ¢, activator protein 1 DNA binding, glutathione biosynthesis, PI3K / Akt, MAPK pathway and the Nrf2 transcription factor that regulates expression of several antioxidant genes. It can be concluded catechinsÂ inhibitsÂ decreasing expression of eNOSÂ at a dose of 6 mg / day and 24 mg / day and can be developed as an agent and vasoprotective ateroprotective.

AL-QURAN DAN SAINS?(BUKTI KEBENARAN DALAM KASUS PROPOLIS MADU) Erna Susanti, MF. Zenrif dan
Khazanah: Jurnal Studi Islam dan Humaniora Vol 12, No 2 (2014)
Publisher : UIN Antasari Banjarmasin

Exposure free radicals in the activity of this life can not be avoided, included exposure free radicals from food additive espesifically Rhodamine B. Carcinogenic effect of Rhodamin B can be caused by Poly Aromatic Hydrocarbons structure that are destructive to the liver as metabolism organ. The purpose of this study was to prove the antioxidant activity of Honey Propolis against liver damage caused by exposure of Rhodamine B as free radical sources. Indicator of liver damage can be indicated by MDA, a marker of oxidative stress that occurs in these organs. The methode was used experimental methode, with seven treatment groups, namely: (1) normal group, (2) the induction of Rhodamin B without propolis therapy group, (3) the induction of Rhodamin B with Honey Propolis therapy with three dose levels are 350 mg, 700 mg and 1050 mg that are given at intervals of two hours after the administration of Rhodamine B (4) Honey Propolis therapy groups are given at interval of 1 week aftertheadministrationof RhodamineBwiththreedoselevels.MDAlevelsweremeasuredbyTBARS method. The results showed that the levels of Malondialdehyde declined steadily in the group treated with honey propolis to less than control groups. There are significant differences in MDA levels between the treatment groups Honey Propolis therapy (p = 0.034, p <0.05). Based on these results, it can be concluded that Honey propolis dose 700 mg act as therapeutic agent in liver damage due to exposure of Rhodamine B significantly.The results ofthis studyprove thetruth ofthe Quraninthe QS. Al-Nahl (16): 69of thewonders ofhoneyin preventingliver damagedue toexposure tohazardous substancesin food.

Efek Ateroprotektif dan Vasoprotektif Katekin Teh Hijau terhadap Ekspresi eNOS pada Tikus Wistar Jantan dengan Diet Tinggi Lemak Erna Susanti Retty Ratnawati
Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Vol. 2 No. 1 (2012): Januari
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/far.v2i1.1155

Catechins , isolate from green tea GMB4 clones could be developed as a potential preventive agent for atherosclerosis. This activity is thought to be based on the structure epimerisation, hydroxy phenolic and galloil group of these isolates. The purpose of this study is to prove the influence catechins ,isolate from green tea, on the inhibition of the decrease eNOS expression in male wistar rats induced by high fat diet. The method used in this study consisted of ELISA for measurement of eNOS expression. The results showed that catechins inhibits the decreasing expression of eNOS at a dose of 6 mg / day and 24 mg / day was significantly but not dose 3mg / day. This suggests a dose 6mg / day and 24 mg / day is able to repair the damage signaling insulin on PI3K pathway caused by insulin resistance. These results are based on the ability of catechins as antioxidants. Catechins with hydroxy phenolic group is a potential electron donor to bind free radicals. Polyphenol catechins may also modulate signaling cellular processes such as NFkâ, activator protein 1 DNA binding, glutathione biosynthesis, PI3K / Akt, MAPK pathway and the Nrf2 transcription factor that regulates expression of several antioxidant genes. It can be concluded catechins inhibits decreasing expression of eNOS at a dose of 6 mg / day and 24 mg / day and can be developed as an agent and vasoprotective ateroprotective.

EFEK KEFIR TERHADAP GAMBARAN HISTOLOGIS DAN KADAR MALONDIALDEHIDE HEPAR MENCIT PUTIH (MUS MUSCULUS) JANTAN GALUR BALB C DENGAN PAPARAN FORMALIN Erna Susanti Ni Made Dwi Aryantini
Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Vol. 2 No. 1 (2012): Januari
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/far.v2i1.1158

Kefir is a probiotic drink act as therapeutic agents in liver damage caused by exposure of formaldehyde. Therapeutic activities were estimate based on the content of kefir like antioxidants, lactic acid bacteria with yeasts, and essential amino acids. The purpose of this study was to prove the influence of kefir on the histological and levels of malondialdehyde (MDA) in liver of white mice (Mus musculus) male Balb C strain that exposure by formalin. The method used is experimental method, with three treatment groups, namely: (1) normal group, (2) the induction of formaline without kefir therapy group, (3) the induction of formaline with kefir therapy group. Histological of liver viewed by HE staining and MDA levels were measured by TBARS method. There are differences in the average number of significant cell damage induced by formalin group (p = 0030, p < 0.05). There are significant differences in MDA levels induced by formalin group (p = 0023, p < 0.05). These results are based on the ability of vitamin C, E and carotene (antioxidant) against Reactive Oxygen Species (ROS) due to exposure of formaldehyde, thus preventing the MDA levels increasing. Lactic acid bacteria and yeasts can preclude the possibility of healthy cells to be attacked by ROS with increasing the body’s immunity. Essential amino acids can repairing damaged cells by stimulating cell regeneration. It can be concluded that kefir with a dose of 107 CFU / ml act as therapeutic agents in liver damage due to exposure to formaldehyde.

The Effect of Green Tea on the Expression of NPC1L1, ABCG5, and ABCG8 in the Intestine of High Fat Diets-induced Rats Erna Susanti; Endang Susilowati
The Indonesian Biomedical Journal Vol 13, No 2 (2021)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v13i2.1420

BACKGROUND: Signaling pathways contributing to cholesterol efflux and inhibitory inflammation in atherosclerosis that has not been explored is the liver X receptor (LXR). Catechin as LXR agonist influences the expression of Niemann-pick C1 like 1 (NPC1L1) protein transporter that triggers the inhibition of cholesterol absorption. This study aimed to examine the effect of Catechins on the expression of intestinal transporters: NPC1L1, ATP-binding cassete-proteins G5 (ABCG5) and G8 (ABCG8).METHODS: Twenty-five experimental animals were divided into five treatment groups, with 5 rats in each group. The groups were normal diet rats (group 1), high fat diets-induced rats (group 2), high fat diets-induced rats treated with 30 mg/kg body weight (BW) of Catechins (group 3), high fat diets-induced rats treated with 60 mg/kg BW of Catechins (group 4), and high fat diets-induced rats treated with 120 mg/kg BW of Catechins (group 5). After one-month, all rats were sacrificed, blood and intestine were collected. Lipid profile were determined enzymatically, mRNA levels were determined by reversetranscriptase polymerase chain reaction (RT-PCR), while the expression of protein transporter were determined by immunohistochemistry.RESULTS: Catechins treatment decreased the expression of NPC1L1, but increased the expression of ABCG5 and ABG8.CONCLUSION: Catechins can be developed as a candidate for NPC1L1 inhibitor to mediate the inhibiting absorption of intestinal cholesterol, therefore increasing the inhibitory effect of atherogenesis.KEYWORDS: ABCG5, ABCG8, aterogenesis, Catechins, green tea, NPC1L1

Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line Roihatul Mutiah; Trian Sidha Minggarwati; Risma Aprinda Kristanti; Erna Susanti
Indonesian Journal of Cancer Chemoprevention Vol 10, No 3 (2019)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev10iss3pp131-139

Eleutherine palmifolia (L.) Merr. is a typical plant found in Central Kalimantan that has been used empirically by the Dayak people as medicine for various diseases, including cancer. The plant contains flavonoid compounds that potentially used as an anticancer. The purpose of this study is to find the most active fraction, indicated by its cytotoxic potency on HeLa cervical cancer cell line, and to identify compounds in E. palmifolia bulbs fraction. E. palmifolia bulbs was extracted by maceration. The extraction with ultrasonic bath and partition fractionation was conducted by using n-hexane, chloroform, and ethyl acetate. Each fraction was tested for toxicity level on HeLa cells using MTT assay. The identification of active compounds was carried out by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS). The result showed that based on the IC50 value, the ethyl acetate fraction had the highest bioactivity. IC50 values of n-hexane, chloroform, and ethyl acetate fractions were 250.77±19.01; 720.46±42.38; and 44.34±9.45μg/mL, respectively. The identification of the active compound in ethyl acetate fraction resulted 28 chemical compounds. Compounds with the highest percentage area were isoliquiritigenin and oxyresveratrol. The ethyl acetate fraction of E. palmifolia bulbs is potential to be developed as an anticancer candidate (phytopharmaceutical).Keywords: Compound identification, Anticancer activity, Eleutherine palmifolia (L.) Merr., cervical cancer

Catechins inhibit atherosclerosis in male rats on a high fat diet Erna Susanti; Achmad Rudijanto; Retty Ratnawati
Universa Medicina Vol. 31 No. 2 (2012)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2012.v31.81-87

BackgroundA catechin isolate from the green tea clone GMB 4, which shows antioxidant activity, may be a candidate drug for prevention of atherosclerosis. The aim of this study was to analyze the effect of catechin on endothelial nitric oxide synthase (eNOS) and p110 phosphoinositide 3-kinase inhibitor (PI3K) expression and on p38 mitogen activated protein kinase (MAPK) activity in male rats fed a high fat diet.MethodsTwenty five male Wistar rats were divided into the following five groups: rats on standard diet; rats on high fat diet; rats on high fat diet + catechin 3 mg/day; rats on high fat diet + catechin 6 mg/day; and rats on high fat diet + catechin 24 mg/day. eNOS and p110 PI3K expression and p38 MAPK activity were measured by means of ELISA. ResultsHigh fat diet significantly increased eNOS expression, decreased p110 PI3K expression, and increased p38 MAPK activity in male rats, in comparison with standard diet (p<0.05). Administration of 3 mg/day catechin decreased eNOS expression compared to that in the high fat diet group without catechin (p<0.05). The administration of catechin increased p100 PI3K expression to a similar extent as that in the high fat diet groups with catechin 6 mg/day and 24 mg/day. Administration of catechin at all doses decreased p38 MAPK activity to the level of the standard diet group. ConclusionsHigh fat diet increases eNOS expression, decreases PI3K expression, and increases p38 MAPK activity. Administration of catechin decreases eNOS expression, increases PI3K expression, and decreases p38 MAPK activity.

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