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All Journal EKSAKTA: Journal of Sciences and Data Analysis Pharmaciana: Jurnal Kefarmasian JKKI : Jurnal Kedokteran dan Kesehatan Indonesia
Kuswati, Kuswati
Departemen Anatomi Fakultas Kedokteran Universitas Islam Indonesia
Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Pengaruh Durasi Iskemia terhadap Ekspresi Protein P53 Otak Tikus Pasca Transient Bilateral Common Carotid Artery Occlusion (TBCCAO) Ety Sari Handayani; Kuswati Kuswati; Zainuri Sabta Nugaha; Nurul Hidayah; Nesti Herennadia; Gea Sonia Amanda; Salsabila Ajeng
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 19, ISSUE 2, August 2019
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/eksakta.vol19.iss2.art3

Abstract

Tumor suppressor gene p53 is one of the specific parameters of the occurrence of brain neuron death in animal models of brain ischemia. Several studies show that the duration of reperfusion of tBCCAO has an effect on the expression of p53 protein. The duration of ischemia for 5 minutes followed by reperfusion of 1 hour, 4 hours, 8 hours, 24 hours, and 7 days increased the expression of p53 protein. 30-minute ischemia induction followed by 4-hour reperfusion showed increased p53 protein expression in rat serum. It is not yet known how the influence of tBCCAO ischemia duration on p53 expression in rat brain. This study used Wistar, male rats, aged 3-4 months, weighing 175-250 gr, and healthy. Group A was a group of tBCCAO rats with a duration of 5 minutes ischemia, 24-hour reperfusion duration (five). Group B was a group of tBCCAO mice with a duration of 10 minutes ischemia, 24-hour reperfusion (five). Group C is a group of sham operated mice (five). The expression of p53 protein is semi quantification of p53 expressed on pyramidal neuron of the frontal brain (Cortex Prefrontalis, striatum) and CA1 hippocampus, with IHC staining using anti-p53 antibodies. P53 expression will be seen in the cytoplasm of pyramidal neuron CA1 hippocampus. Cytoplasm of neurons will be brownish in color. The semi-quantification method of p53 expression uses the ALLRED score. Data analysis using one way ANOVA test. The analysis found differences in frontal brain p53 expression (cortex prefrontalis and striatum) (p = 0.00) and there were differences in p53 expression in pyramidal neuron CA1 hippocampal (p = 0.013). There was an effect of the duration of tBCCAO on expression of p53 protein in rat brain after 24-hour reperfusion. Keywords: tBCCAO’s duration, p53, rat’s brain
P53 expression in ischemic rat models after the administration of ketamine and ketamine-xylazine Ety Sari Handayani; Zainuri Sabta Nugraha; Kuswati Kuswati; Muhammad Yusuf Hisyam; Untung Widodo; Nurul Hidayah; Sahdella Sahdella; Wimpy Wimpy
Pharmaciana Vol 10, No 1 (2020): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.017 KB) | DOI: 10.12928/pharmaciana.v10i1.13451

Abstract

Ketamine and ketamine-xylazine are often used as anesthetic drugs in animal models of ischemia. However, their neuroprotective and neurotoxic effects in ischemic animal models that have undergone tBCCAO are still under debate. The protein p53 is a pro-apoptotic factor involved in the cellular mechanism of ischemia. The interaction between death-associated protein kinase 1 (DAPK 1) and p53 is fundamental in determining whether cells experience necrosis or apoptosis in an ischemic stroke. This study was purposed to identify the presence or absence of differences between the p53 expressions in the brains of tBCCAO-induced ischemic rat models after the administration of ketamine and ketamine-xylazine. It employed a post-test control group design with four groups of adult male Wistar rats as the subject: (1) sham group operated with ketamine, (2) sham group operated with ketamine-xylazine, (3) models of tBCCAO-induced ischemia with ketamine, and (4) models of tBCCAO-induced ischemia with ketamine-xylazine. Ketamine was administered at the dose of 75mg/kg BW, while xylazine was at 8 mg/kg BW. The expression of p53 in rat brains was assessed by semi-quantification, specifically IHC staining with anti-p53 antibodies. P53 expression appeared as brownish stains in the cytoplasm of forebrain pyramidal neurons, and in this study, it was measured using the Allred score. The ANOVA test yielded a p-value of >0.05, implying the absence of difference between the p53 expressions in the brains of tBCCAO-induced ischemic rat models receiving ketamine and ketamine-xylazine.
PENGARUH EKSTRAK ETANOL Centella asiatica TERHADAP JUMLAH SEL NEURON DI KORTEKS PREFRONTALIS TIKUS YANG DIBERI PERLAKUAN STRES Ayu Kurnia Priyantiningrum; Kuswati -; Ety Sari Handayani
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 6, No 4, (2015)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol6.Iss4.art5

Abstract

Latar Belakang Stres berdampak pada penurunan fungsional tubuh yang dapat menimbulkan suatu penyakit seperti ketidakmampuan kognitif yang serius, diketahui bahwa kemampuan kognitif diatur pada Korteks Prefrontalis di otak. Respon tubuh terhadap stress berupa kemampuan untuk bertahan hidup hingga apoptosis. Centella asiatica merupakan tanaman obat yang memiliki senyawa triterpen sebagai antioksidan yang mampu memproteksi sel untuk dapat terhindar dari apoptosis. Tujuan Penelitian Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak etanol pegagan (Centella asiatica) terhadap jumlah sel neuron di korteks prefrontalis tikus putih (Rattus norvegicus) yang diberi perlakuan stres Metode Penelitian Penelitian ini merupakan penelitian eksperimental dengan menggunakan rancangan post test-with control group. Subyek penelitian ini menggunakan 12 tikus dewasa (Rattus norvegicus) galur Wistar yang memenuhi kriteria inklusi dan eksklusi. Subyek terbagi sama rata menjadi kelompok kontrol dan perlakuan. Kedua kelompok dilakukan stres restrain selama 21 hari. Tiga puluh menit sebelum stress, kelompok kontrol diberikan Pulvis Gum Arabica 3%. Kelompok perlakuan diberikan ekstrak etanol pegagan 300 mg/kgBB/hari. Perbedaan jumlah sel neuron rerata pada seluruh lapang pandang setiap kelompok dianalisis dengan uji T-test independent. Hasil Hasil penelitian ini menunjukkan bahwa jumlah sel neuron rerata di Korteks Prefrontalis setelah pemberian ekstrak etanol pegagan (Centella asiatica) lebih banyak dibandingkan kelompok kontrol (p-value 0,004; CI 95%). Kesimpulan Pemberian ekstrak etanol pegagan (Centella asiatica) signifikan meningkatkan jumlah sel neuron di Korteks Preftontalis tikus (Rattus norvegicus) yang diberi perlakuan stres, dibanding tikus kontrol yang tidak diberikan pegagan Kata Kunci: Stres Restrain, Centella asiatica, Korteks Prefrontalis, Rattus norvegicus
PENGARUH PEMBERIAN PROPOLIS TERHADAP EKSPRESI BDNF DI HIPPOCAMPUS TIKUS YANG DIINDUKSI STRES Kuswati Kuswati; Zainuri Sabta Nugraha; Ety Sari Handayani
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 12, No 3, (2021)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol12.Iss3.art8

Abstract

Background: Brain-derived neurotrophic factor (BDNF) is a neurotrophin secreted by dendrite, which plays a role in differentiation, maturation, neuroplasticity, learning, and memory, which the expression decreases under stress conditions. Propolis contains chrysin which has antioxidant and neuroprotectant effects.Objective: This study aimed to examine the effect of propolis on BDNF expression in the hippocampus of stress-induced rats.Methods: Experimental study using posttest only control group design. The subjects were 25 male Spraque-Dawley rats (Rattus norvegicus), four months old, weighing 200-300 grams. Rats were randomly divided into five groups: group N, did not receive any treatment; group K, received stress treatment; groups P1, P2, and P3, received stress treatment and followed by administered propolis at doses of 100, 150, and 200 mg/kg/day. Social isolation stress was carried out by putting one rat in one cage. Oral propolis administration used oral gavage. In the end, the rats were terminated and brain tissue was collected. Immunohistochemical staining using anti-BDNF antibodies was performed to make histological slides. Observations were made with a light microscope with 1000x magnification in the CA1 area of the hippocampus.Results: There is a significant difference in BDNF expression in the CA1 area of the hippocampus in all groups (p=0.000). The highest BDNF expression was in the P3 group and the lowest in group K.Conclusion: There is an effect of propolis on BDNF expression in the hippocampus of the stress-induced rat. Propolis dose of 100 mg/kgBW/day has increased BDNF expression.
Co-Authors Ayu Kurnia Priyantiningrum Ety Sari Handayani Gea Sonia Amanda Muhammad Yusuf Hisyam Nesti Herennadia NURUL HIDAYAH Nurul Hidayah Sahdella Sahdella Salsabila Ajeng Untung Widodo Wimpy Wimpy Zainuri Sabta Nugaha Zainuri Sabta Nugraha