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All Journal EKSAKTA: Journal of Sciences and Data Analysis Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Pharmaciana: Jurnal Kefarmasian Universa Medicina Jurnal Kedokteran Anatomica (Anatomica Medical Journal) Healthy-Mu Journal Refleksi Pembelajaran Inovatif JKKI : Jurnal Kedokteran dan Kesehatan Indonesia
Zainuri Sabta Nugraha
Departemen Anatomi Fakultas Kedokteran Universitas Islam Indonesia
Humanities Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences

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The Effect of Ethanol Extract of Arabica Coffee (Coffea Arabica Linn) on the Number of Adult Pyramidal Pallium Zebrafish (Danio rerio) Neurons exposed to Stress Method UCS (Unpredictable Chronic Stress) Muchamad Marwan; Zainuri Sabta Nugraha
Healthy-Mu Journal Vol. 5 No. 1 (2021)
Publisher : MBUnivPress

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35747/hmj.v5i1.33

Abstract

Chronic stress can have an adverse effect on behavior changes and damage to the pyramidal neurons of the brain. Pyramidal neurons of the brain play an important role in awareness, decision making, cognitive function, spatial memory formation and working memory. The effects of chlorogenic acid (CGA) found in arabica coffee can affect the improvement of cognitive function, working memory, and histology of brain pyramidal neurons. The content of chlorogenic acid (CGA) in coffee has active compounds that can maintain the number of brain pyramidal neurons due to stress. The aim of this study was to determine the effect of arabica coffee extract on the average number of brain pyramidal neurons exposed to UCS stress. The research method used was a post-test experimental study with controlled group design. A total of 24 adult zebrafish (3 months old) were divided into 4 groups. Four groups (KN, KP, K25, K50) were acclimatized (1 week) first, for groups K25 and K50 were given arabica coffee extract as much as 25mg / dl and 50 mg / dl for 4 days before inducing unpredictable chronic stress (UCS). The results of the study proved that the extra ethanol group of arabica coffee at a dose of 50 mg / dl could maintain the number of pyramidal neurons in zebrafish treated with modified UCS stress (P <0.05). In conclusion, giving ethanol extract of arabica coffee at a dose of 50 mg / dl has a neuroprotectant effect on zebrafish exposed to UCS stress.
Binaural entrainment of 2000-2040 Hz and 2000-2090 Hz increase Glial Fibrillary Acidic Protein (GFAP) expression of astrocytes in the CA1 rat hippocampus during operant learning conditioning Zainuri Sabta Nugraha; Soedjono Aswin; . Harijadi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 03 (2014)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.092 KB) | DOI: 10.19106/JMedScie004603201401

Abstract

Astrocytes of hippocampus contribute in the learning performance. Entrainment of gamma wavescan improve learning performance by improving the neurons to astrocytes communication. Theaim of this study was to evaluate the effect of binaural entrainment of 2000-2040 Hz and 2000-2090 Hz on Glial Fibrillary Acidic Protein (GFAP) expression in astrocytes of the CA1 region ofrat hippocampus during operant learning conditions. Twenty male Wistar rats aged 4-6 weekswith body weight 100-150 g were devided into 4 groups. Group I was given binaural soundsentrainment at 2000-2040 Hz without learning test. Group II was given entrainment as performedin Group I followed by a learning test. Group III was given binaural sounds entrainment at 2000-2090 followed by a learning test. Group IV was not given entrainment nor learning test. Theentrainment was performed for 30 minutes everyday for 12 days and the learning test wasperformed for 10 minutes everyday for 12 days. The GFAP expression was examinedimminohistochemically. Astrocytes processes and astrocytes histoscore were also calculated.The results showed that the number of the GFAP-positive astrocytes in Group I (70.96 ± 4.86),II (69.76±3.07) and III (63.10±5.85) were significantly higher than Control (47.33±1.33)(p<0.05). The number of the processes astrocytes in Group I (47.64 ± 3.87), II (60.66±2.07)and III (54.17±6.38) was significantly higher than Control (30.87±2.69) (p<0.05). Moreover,the number of the processes astrocytes in Group II was significantly higher than Group I (p=0.016).The astrocytes histoscore index in the Group II (115.58±14.13) and III (78.32±22.23) weresignificantly higher than Group I (28.79 ± 9.61) and Control (16.05±1.64) (p<0.05). Inconclusion, the binaural entrainment of 2000-2040 Hz and 2000-2090 Hz increase GFAP expressionof astrocytes in Cornu Ammonus 1 (CA1) region of rat hippocampus during operant learningconditioning.
P53 expression in ischemic rat models after the administration of ketamine and ketamine-xylazine Ety Sari Handayani; Zainuri Sabta Nugraha; Kuswati Kuswati; Muhammad Yusuf Hisyam; Untung Widodo; Nurul Hidayah; Sahdella Sahdella; Wimpy Wimpy
Pharmaciana Vol 10, No 1 (2020): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.017 KB) | DOI: 10.12928/pharmaciana.v10i1.13451

Abstract

Ketamine and ketamine-xylazine are often used as anesthetic drugs in animal models of ischemia. However, their neuroprotective and neurotoxic effects in ischemic animal models that have undergone tBCCAO are still under debate. The protein p53 is a pro-apoptotic factor involved in the cellular mechanism of ischemia. The interaction between death-associated protein kinase 1 (DAPK 1) and p53 is fundamental in determining whether cells experience necrosis or apoptosis in an ischemic stroke. This study was purposed to identify the presence or absence of differences between the p53 expressions in the brains of tBCCAO-induced ischemic rat models after the administration of ketamine and ketamine-xylazine. It employed a post-test control group design with four groups of adult male Wistar rats as the subject: (1) sham group operated with ketamine, (2) sham group operated with ketamine-xylazine, (3) models of tBCCAO-induced ischemia with ketamine, and (4) models of tBCCAO-induced ischemia with ketamine-xylazine. Ketamine was administered at the dose of 75mg/kg BW, while xylazine was at 8 mg/kg BW. The expression of p53 in rat brains was assessed by semi-quantification, specifically IHC staining with anti-p53 antibodies. P53 expression appeared as brownish stains in the cytoplasm of forebrain pyramidal neurons, and in this study, it was measured using the Allred score. The ANOVA test yielded a p-value of >0.05, implying the absence of difference between the p53 expressions in the brains of tBCCAO-induced ischemic rat models receiving ketamine and ketamine-xylazine.
Soursop leaf extract increases neuroglia and hepatic degeneration in female rats Ety Sri Handayani; Zainuri Sabta Nugraha; Prilly i Raleka Pahlevawati
Universa Medicina Vol. 34 No. 1 (2015)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2015.v34.17-24

Abstract

BACKGROUNDSoursop leaf contains annonaceous acetogenins and alkaloids. Theacetogenins act as inhibitors of mitochondrial complex I, suppress ATPproduction and cause cell degeneration, whereas the alkaloids act asneurotoxins. Neuronal degeneration will be followed by an increase inneuroglia (gliosis). Hepatic clear cell foci represent the morphology of liverdegeneration. The purpose of this study was to evaluate the effect of soursopleaf extract on number of neuroglia brain gliosis and hepatic clear cells infemale rats.METHODSThis study was an experimental study with a post-test only control groupdesign. Ten female Sprague-Dawley strain rats were divided into one controland one treatment group. The control group was gavaged with distilled water,while the treatment group was gavaged with aqueous soursop leaf extract ata dose of 1000 mg/kgBW/day for 90 days. Rat brain tissue samples weretaken at day 91 with a transcardial perfusion technique. The number ofneuroglia in rat cerebral cortex, hippocampus, substantia nigra, and nucleusaccumbens and the number of hepatic clear cells were determined.Independent t-test was used to examine the differences in the numbers ofneuroglia and hepatic clear cells between control and treatment groupsRESULTSThe results of independent t-test analysis found a significant difference inthe number of neuroglia in the cerebral cortex (p=0.015) and nucleusaccumbens of the rats (p=0.030), and significant differences in the numberof hepatic clear cells (p=0.029).CONCLUSIONSAqueous soursop leaf extract orally increases neuroglia of the cerebral cortexand nucleus accumbens, and hepatic degeneration in female rats.
The Effect of Subchronic Administration of Soursop (Annona muricata) Leaf Aqueous Extract In Bax Expression on Gastric Glandular and Non Glandular Mucosal Epithelium of Rat (Rattus norvegicus) Ety Sari Handayani; Zainuri Sabta Nugraha; Chandra Muhammad Rauf; Yusa Muhammad Thoriq; Nurul Hidayah
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 3, ISSUE 1, February 2022
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/EKSAKTA.vol3.iss1.art7

Abstract

Soursop leaf water extract (SLWE) contains acetogenin compounds which are mitochondrial complex I inhibitors. This compound can reduce cellular ATP production and induce apoptosis via bax pathways, causing side effects on cells. Gaster is a digestive organ that has direct contact with acetogenin. Gastric cells contain mitochondria and undergo physiological apoptosis.This study aims to determine the effect of subchronic administration of SLWE on bax expression on glandular/non glandular mucosal epithelium gastric of rats. This study was conducted at Research Laboratorium, Medical Faculty, UII, 2019. This study uses post test only control group design. The subjects were 10 female rats, Spraque-dawley strain which was divided into 2 groups; the treatment and the control group. The treatment group received extract of SLWE at a dose of 1000 mg/kgBB/day for 30 days, while the control group received aquades, both were administered using sonde. Observation of bax expression was performed on each IHC preparation with bax antibody. The difference in bax expression between the control and treatment groups was tested by t-test. There were significant differences in the number of bax expressions in gastric glandular mucosa (p 0.038) and non-glandular gastric mucosa (p 0.027) between the treatment group and the control group. There was an effect of subchronic administration of SLWE on bax expression on mucosalepithelium for both, glandular and non-glandular of rat gaster. There were differences in the number of glandular gastric as well as non-glandular gastric mucosa epithelium which exerted bax between the control and treatment groups
Refleksi pembelajaran anatomi pada mahasiswa kedokteran fase ketiga melalui applied and clinical question Zainuri Sabta Nugraha; Fuad Khadafianto; Ika Fidianingsih
Refleksi Pembelajaran Inovatif Vol. 1 No. 1 (2019): Volume 1 Nomor 1 Tahun 2019
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/rpi.vol1.iss1.art3

Abstract

Latar belakang Anatomi merupakan ilmu dasar yang sangat penting bagi mahasiswa kedokteran. Walaupun pengajaran anatomi telah diselenggarakan pada semester awal, namun pada praktek klinik, mahasiswa kesulitan untuk mengaplikasikan ilmu ini pada suatu kasus. Untuk itu kombinasi metode yaitu recalling melalui minikuis disertai kasus klinik (applied and clinical question) sangat urgen. Metode Peserta yang mengikuti program ini adalah mahasiswa semester VI, Program studi Pendidikan Dokter Fakultas Kedokteran Universitas Islam Indonesia. Applied and clinical anatomy question disusun setiap minggu sebanyak 1 sampai 2 soal, dan pada akhir blok dalam ujian blok sebanyak 8 sampai dengan 11 soal. Total soal berjumlah 53 soal. Pada awal setiap blok dilakukun pretes sebanyak antara 11 sampai dengan 19 soal, total soal 50. Pada pertengahan berjalannya blok, departemen Anatomi memberi kuliah contoh contoh soal antomi klinik dan pembahasan, dan mendorong kembali mahasiswa mempelajari anatomi klnik secara mandiri. Setelah selesai blok (akhir semester) peserta mengikuti postes sebanyak 50 soal. Kuasioner dan focus group discussion mahasiswa untuk evaluasi. Hasil Rata-rata nilai pretes anatomi klinik dibanding postes meningkat yaitu 39,9(±7,97) menjadi 47,41 (±8,47) dengan nilai p=0,00. Sebanyak 95,29% mahasiswa menyadari pentingnya anatomi untuk memahami penyakit. Sebanyak 84,70% menyatakan Applied and clinical question untuk anatomi bermanfaat. Kesimpulan. Mahasiswa menyadari pentingnya anatomi untuk memahami penyakit. dan menyatakan Applied and clinical question untuk anatomi bermanfaat. Applied and clinical question dapat meningkatkan pemahaman anatomi (p=0,00). Namun adanya tujuan belajar yang lebih rinci untuk fase lanjut terkait anatomi klinik untuk panduan belajar anatomi secara mandiri perlu disiapkan.
PENGARUH MONOSODIUM GLUTAMAT TERHADAP TAMPILAN BELAJAR KONDISI OPERAN TIKUS PUTIH (Rattus norvegicus) GALUR WISTAR Kinasih D S; Zainuri Sabta Nugraha
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 5, No 2, (2013)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

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Abstract

Latar BelakangSampai saat ini masyarakat masih menggunakan zat aditif dalam mengkonsumsi makanan sehari-hari seperti Monosodium Glutamat (MSG). Efek MSG terhadap kualitas belajar masih diperdebatkan. Beberapa penelitian menyatakan bahwa MSG dapat menurunkan kualitas belajar, namun beberapa penelitian lain tidak memberikan efek kualitas belajar.TujuanUntuk mengetahui bagaimana pengaruh MSG terhadap tampilan belajar tikus putih (Rattus norvegicus) galur Wistar.MetodePenelitian ini merupakan penelitian eksperimental sederhana dengan rancangan pretestposttest controlled group design. Tikus putih sebanyak 18 ekor jantan dan betina dibagi menjadi 3 kelompok masing-masing 6 ekor sebagai kontrol dan perlakuan dengan diberikan MSG 120 mg/hari dan 240 mglhari peroral. Masing-masing kelompok diuji bela jar menggunakan skinner box selama 10 menit sebelum dan 4-5 jam setelah pemberian MSG yang kemudian dihitung jumlah reward dan punishment yang didapatkan selama 12 hari pengujian. Analisis menggunakan uji Oneway AN OVA, dan paired t test.HasilRerata reward pada kelompok kontrol, kelompok perlakuan A dan kelompok perlakuan B berturut-turut adalah 0,21; 0,12; 0,18 (p=0,629). Sedangkan rerata punishment bertututturut0,16; 0,15; 0,32 (p=0,169). Berdasarkan hasil penghitungan paired t-test untuk reward dan punishment pada kelompok A sebelum dan sesudah pemberian MSG nilai p berturut-turut 0,585 dan 0,006 sedangkan nilai signifikan untuk reward dan punishment pada kelompok B sebelum dan sesudah pemberian MSG adalah 0,208 dan 0,489.KesimpulanPenelitian ini membuktikan bahwa MSG dengan dosis 120mg/kgBB/hari dan 240mg/kgBB/hari yang diberikan selama 12 hari tidak berpengaruh terhadap tampilan belajar tikus putih (Rattus norvegicus) galur Wistar.
PENGUJIAN TERHADAP KADAR GAS FORMALIN DI RUANG PRAKTIKUM LABORATORIUM ANATOMI FAKULTAS KEDOKTERAN UNIVERSITAS ISLAM INDONESIA (UII) (Studi level Toksisitas Dibandingkan Dengan Standar Nilai Ambang Batas) Raden Edi Fitriyanto; Zainuri Sabta Nugraha
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 1, No 3, (2009)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

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Abstract

Formaline solvent is widely used in life, example as a preservative cadaver in anatomy laboratory. Forma line solvent can evaporate easily, in the form of gas it should be react with mucous layer quickly, especially at respiratory tract. In human body, it is oxidated become formic acid, especially in the cells of liver and red blood. In many earlier researches, the short-term exposure caused irritation to the local mucous surface of eyes, nose and up respiratory tract. However, the long term exposure caused tumor growth in the rat nose, pulmonary t issue damage and asthma reactions in sensitive individuals. The aim of the study is to measure forma line gas concentration and comparing the result with Thresold Limit Value (TLV) that safety for health. The research is nonexsperimental study with associative analysis. The research objects were practice space, storage cadaver space and lobby of Anatomy Laboratory. The research subjects were divided into 3 phases of measurement, phase I is measurement in the athmosphere (control), phase II is measurement in practice space laboratory during activities and phase Ill is measurement in practice space laboratory with no activities. Formaline gas concentration in practice space Anatomy Laboratory is under the TLV (TLV: 0.3 ppm) with no activities. Duringactivities, it is over the TLV: in preparation space 0,99569 ppm and practise space 0,37804 ppm. Formaline gas concentration in practice space during activities is over the TLV. It is a risk to cause some health disorders, especially to user and staff of the laboratory. It need good planning of practise programs and design of anatomy laboratory based on safety.
PENGARUH PEMBERIAN PROPOLIS TERHADAP EKSPRESI BDNF DI HIPPOCAMPUS TIKUS YANG DIINDUKSI STRES Kuswati Kuswati; Zainuri Sabta Nugraha; Ety Sari Handayani
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 12, No 3, (2021)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol12.Iss3.art8

Abstract

Background: Brain-derived neurotrophic factor (BDNF) is a neurotrophin secreted by dendrite, which plays a role in differentiation, maturation, neuroplasticity, learning, and memory, which the expression decreases under stress conditions. Propolis contains chrysin which has antioxidant and neuroprotectant effects.Objective: This study aimed to examine the effect of propolis on BDNF expression in the hippocampus of stress-induced rats.Methods: Experimental study using posttest only control group design. The subjects were 25 male Spraque-Dawley rats (Rattus norvegicus), four months old, weighing 200-300 grams. Rats were randomly divided into five groups: group N, did not receive any treatment; group K, received stress treatment; groups P1, P2, and P3, received stress treatment and followed by administered propolis at doses of 100, 150, and 200 mg/kg/day. Social isolation stress was carried out by putting one rat in one cage. Oral propolis administration used oral gavage. In the end, the rats were terminated and brain tissue was collected. Immunohistochemical staining using anti-BDNF antibodies was performed to make histological slides. Observations were made with a light microscope with 1000x magnification in the CA1 area of the hippocampus.Results: There is a significant difference in BDNF expression in the CA1 area of the hippocampus in all groups (p=0.000). The highest BDNF expression was in the P3 group and the lowest in group K.Conclusion: There is an effect of propolis on BDNF expression in the hippocampus of the stress-induced rat. Propolis dose of 100 mg/kgBW/day has increased BDNF expression.
Lokasi Fraktur Femur Tidak Berpengaruh terhadap Tekanan Darah Pasien di RS Islam PKU Muhammadiyah Pekajangan Febiawan Ismunandar; Zainuri Sabta Nugraha; Arie Nugroho
ANATOMICA MEDICAL JOURNAL | AMJ Vol 5, No 2 (2022)
Publisher : Universitas Muhammadiyah Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30596/amj.v5i2.10374

Abstract

Fraktur femur salah satu fraktur ekstremitas yang sering terjadi. Banyaknya vaskularisasi pada regio femur menyebabkan syok hipovolemik ketika fraktur. Salah satu parameter syok yang bisa dilihat yaitu tekanan darah yang dianggap sebagai perubahan dinamik. Penelitian ini bertujuan untuk mengetahui pengaruh lokasi fraktur femur terhadap tekanan darah pasien di RS Islam PKU Muhammadiyah Pekajangan. Metode: Penelitian ini merupakan studi observasional analitik dengan desain cross-sectional. Data yang diperoleh merupakan data rekam medis pasien fraktur femur pada tahun 2016-2020 di RS Islam PKU Muhammadiyah Pekajangan. Data kemudian dianalisis menggunakan SPSS dengan uji one-way ANOVA. Hasil: Jumlah data yang diperoleh dalam penelitian ini sebanyak 93 kasus fraktur. Sampel yang memenuhi kriteria inklusi yaitu 48. Fraktur femur proksimal merupakan kasus terbanyak dibandingkan fraktur distal dan medial. Hasil analisis pengaruh lokasi fraktur femur terhadap tekanan darah menggunakan uji one-way ANOVA didapatkan (p-value = 0.254) untuk sistol dan (p-value = 0.642) untuk diastol. Hal tersebut berarti bahwa pengaruh lokasi fraktur femur terhadap tekanan darah adalah sama secara signifikan. Kesimpulan: Kesimpulan dari penelitian ini didapatkan bahwa lokasi fraktur femur tidak berpengaruh terhadap tekanan darah pasien.Kata kunci : fraktur femur, lokasi fraktur, tekanan darah.
Co-Authors . Harijadi Arie Nugroho Chandra Muhammad Rauf Ety Sari Handayani Ety Sri Handayani Febiawan Ismunandar Fitriyanto, Raden Edi Fuad Khadafianto Ika Fidianingsih Kinasih D S Kuswati Kuswati Kuswati, Kuswati Muchamad Marwan Muhammad Yusuf Hisyam Nurul Hidayah NURUL HIDAYAH Prilly i Raleka Pahlevawati Sahdella Sahdella Soedjono Aswin Untung Widodo Wimpy Wimpy Yusa Muhammad Thoriq Yusi Maulina