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All Journal Dentika Dental Journal Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry

Ardo Sabir, Ardo

Department Of Conservative Dentistry, Faculty Of Dentistry, Universitas Hasanuddin, Makassar

Author-ID : 499371

Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Articles

Rat dental pulp tissue reaction after capped with propolis derived nonflavonoid extract Sabir, Ardo
Padjadjaran Journal of Dentistry Vol 23, No 1 (2011): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Propolis is a resinous material collected by honey bees from various plants. Many types of research have shown that it has antibacterial and anti-inflammation activities. Flavonoid is the main chemical substance in propolis that inhibits bacterial growth and reduces the release of free radicals, suggesting that this component has anti-bacterial and anti-inflammatory properties. However, there is another chemical substance in propolis that shows antibacterial dan anti-inflammatory activities. The purpose of the present study was to assess the rat dental pulp tissue reaction after capped with propolis derived non-flavonoids extract. Non-flavonoids substances were purified from an ethanol extract of propolis obtained from South Sulawesi, Indonesia. A Class I cavity was prepared on the occlusal surface of the right maxillary first molar in Spraque-Dawley rats. The dental pulp was exposed and then capped with a zinc oxide-based filler as a control (Group I), or non-flavonoids propolis (Group II). Then, each cavity was filled with glass ionomer cement. The animals were sacrificed at week 1, 2, or 4. Biopsy samples were obtained, and these were stained and viewed by light microscopy. The histological examination was based on the presence of polymorph nuclear leukocytes and macrophages. The results showed that pulp inflammation occurred in both groups as early as week 1. However, the inflammation occurred in Group II was relatively milder compared to Group I at all time period. Therefore, present results suggest that application of non-flavonoids propolis extract on rats dental pulp tissue might inhibit inflammatory process.

PROSPECT OF USING PROPOLIS IN CONSERVATIVE DENTISTRY AND ENDODONTIC TREATMENT (BASIC RESEARCH VS CLINICAL APPLICATION): PROSPEK PENGGUNAAN PROPOLIS DALAM BIDANG OPERATIVE DENTISTRY DAN PERAWATAN ENDODONTI (PENELITIAN DASAR VS APLIKASI KLINIS Ardo Sabir
Dentika: Dental Journal Vol. 18 No. 2 (2014): Dentika Dental Journal
Publisher : TALENTA

Propolis is a resinous hive product, collected by bees from tree buds and mixed with secreted bee wax in order to avoidbacterial contamination in the hive, and also to seal it. It is acknowledged that propolis has antimicrobial activity as wellas anti-inflammatory, antioxidant, antitumor, immuno-regulatory, and regenerative tissue properties. Chemically, propolisis exceedingly complex and contains a rich variety of potent terpene, benzoic, caffeic, cinnamic, aromatic aldehyde,phenolic acid, and also high in flavonoid. Recently, propolis has been the subject of several studies, both in vitro and invivo or even at animal or human with the aim to elucidate its biological and pharmacological properties. The use ofpropolis in dentistry has been done in few years ago for various purposes such as in treatment of periodontitis and dentalcaries, to accelerate the healing of post-extraction wounds, to treat aphthous stomatitis, as a storage media following teethavulsion. The result of basic research of propolis apparently was useful to support its clinical application in dentistry.However, standardization the composition of propolis due to its chemical diversity is still a problem. Therefore thepurposes of this review article were (1) to explain the prospect of propolis in conservative dentistry and endodontictreatment, and (2) to stimulate the interest of clinicians to make further research about this natural compound in dentistry.In conclusion, propolis is a natural medication with a promising future but further studies should be conducted toinvestigate its merit and demerits in clinical dentistry

Root canal overfilling as an influencing factor for the success of endodontic treatment Ardo Sabir
Dental Journal (Majalah Kedokteran Gigi) Vol. 38 No. 4 (2005): December 2005
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

The goal of endodontic treatment is to keep the teeth as long as possible in the mouth. The obturation process in the root canal is one of the most important processes in endodontic treatment. The purpose of this article is to explain that overfilling is an influencing factor to the success of endodontic treatment. It has been widely known that overfilling should be avoided during an obturation process. Overfilling of the root canal is indicated only in cases which will be followed by apicoectomy, when the foreign material is removed. Accidental overfilling may occur with soft material (for example, certain pastes and cements) or with solid material (such as gutta-percha or silver cones). Such overfilling may cause an unnecessary mechanical and chemical irritation, which hinders the repair of periapical tissue, and thus, diminishes the probability of a successful endodontic treatment prognosis. Many things could cause overfilling, which makes overfilling unpleasant feeling for the patient such as severe pain, periapical lesion, gingival discoloration, periodontal ligament breakage, or even paresthesia. There are several ways to overcome this root canal overfilling, from the conventional endodontic treatment up to endodontic surgery. The main conclusion from this article is to avoid overfilling and the importance of clinicians’ compliance to the right procedures.

Aktivitas antibakteri flavonoid propolis Trigona sp terhadap bakteri Streptococcus mutans (in vitro) (In vitro antibacterial activity of flavonoids Trigona sp propolis against Streptococcus mutans) Ardo Sabir
Dental Journal (Majalah Kedokteran Gigi) Vol. 38 No. 3 (2005): September 2005
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

A number of investigations have shown a positive correlation between the number of Streptococcus mutans (S. mutans) in dental plaque and the prevalence of dental caries. Consequently, this microorganism has been the prime target for the prevention of dental caries. Propolis being a substance made by the honeybee, is a potent antibacterial agent. The main chemical class present in propolis is flavonoids. Flavonoids are well-known plant compounds that have antibacterial property. Because S. mutans is accepted to be one of the microorganisms responsible for dental caries and flavonoids in propolis are antibacterial, the purpose of this study was to evaluate in vitro the antibacterial activity of flavonoids Trigona sp propolis against S. mutans as a first step in its possible use as an alternative anticaries agent. Extract flavonoids was purified from ethanol extract of propolis which was obtained from Bulukumba Regency South Sulawesi using thin layer chromatography. The purification of flavonoids was carried-out by UVradiation at λ max 254 nm and λmax 366 nm and treatment with ammonia. Extract flavonoids was diluted in aquadest to 0.05%; 0.075%; 0.1%; 0.25%; 0.5%; 0.75% concentrations. Aquadest and 10% Povidone iodine were also used as control solution. S mutans were grown in medium glucose nutrient agar and incubated with flavonoids for 24 and 48 hours, at 37° C. Antibacterial activity was reflected by the diameter of the inhibition zones around the stainless steel cylinder. The data were analyzed by using ANOVA followed by LSD test with significance level of 5%. The results of this study showed that after being incubated for 24 and 48 hours, all flavonoid concentrations significantly (p < 0.05) inhibited the growth of S mutans. 0.1% flavonoid was the most effective concentration to inhibit the growth of S mutans after 24 hours of incubation and 0.5% flavonoid after 48 hours of incubator.

Respons inflamasi pada pulpa gigi tikus setelah aplikasi ekstrak etanol propolis (EEP) (The inflammatory response on rat dental pulp following ethanolic extract of propolis (EEP) application) Ardo Sabir
Dental Journal (Majalah Kedokteran Gigi) Vol. 38 No. 2 (2005): June 2005
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Propolis is a resinous hive product collected by bees from tree buds and mixed with secreted bee wax in order to avoid bacterial contamination in the hive, and also to seal it. Propolis is employed for the treatment of various infectious diseases because it is wellknown that is has antibacterial and anti-inflammatory properties. The therapeutic use of propolis in dentistry has been done in recent years as in treatment gingivitis and dental caries, in treatment wound healing after surgical procedures, etc. The purpose of this investigation was to study the inflammatory response on rat's dental pulp following application of ethanol extract of propolis (EEP). Twelve male Spraque-Dawley rats of 8–16 week old and 200–250 grams in weight were used in this study. The rats were randomly divided into two groups. Pulp exposures were performed on the occlusal surface of right maxillary first molars. At the first group, as the control group, zinc oxide-based filler was directly applied on pulp exposure. Meanwhile at the second group, as the sample group, pulp exposure was applied with EEP. After that, all cavities were filled with glass ionomer cement as permanent filling. Animals were sacrificed on the 7th, 14th, and 28th day. The criterion of histological examination was based on the inflammatory responses of the dental pulp, which were the presence of polymorph nuclear leukocytes and macrophages. Kruskall-Wallis and Mann-Whitney tests were employed to analyze the data. The results of this research demonstrated that the inflammatory response of EEP group was numerically milder compared to the control group, even though statistical analysis showed no significant difference (p > 0.05) between the two groups.

Co-Authors I Made Jawi Putu Astawa Wiwekowati

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