Ani Widiastuti, Ani
Fakultas Pertanian Universitas Gadjah Mada

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METODE ISOLASI PYRICULARIA ORYZAE PENYEBAB PENYAKIT BLAS PADI Wicaksono, Danar; Wibowo, Arif; Widiastuti, Ani
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 1 (2017): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (235.481 KB) | DOI: 10.23960/j.hptt.11762-69

Abstract

Isolation method of Pyricularia oryzae the causal agent of rice blast disease. Rice blast disease is a disease that reduces rice productivity and threatens global food reserves. The study of diversity and distribution of race fungal causing rice blast disease required a fungal isolates collection from different places and times. One of the challenges in collecting these fungi is the difficulty of isolation process. The purpose of this research was to study the proper isolation method of rice blast pathogen. The most appropriate isolation method of Pyricularia oryzae was to moisten the infected panicle, place on moist filter paper in a petri dish, and incubate plate for 2 days at room temperature under fluorescent lamp. Afterward, conidium was picked using sterile needle and transfered to potato dextrose agar without lactic acid.
PENGEMBANGAN BAHAN AJAR BENTUK-BENTUK GEOMETRI BERBASIS CERITA UNTUK ANAK USIA 5-6 TAHUN DI TK MAZHARUL IMAN PALEMBANG Widiastuti, Ani; Rahelly, Yetty; Sayfdaningsih, Sayfdaningsih
Jurnal Pendidikan Anak Vol 7, No 2 (2018): Jurnal Pendidikan Anak
Publisher : Fakultas Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (386.403 KB) | DOI: 10.21831/jpa.v7i2.24461

Abstract

Penelitian ini bertujuan untuk mengembangkan bahan ajar berupa buku. Pengembangan bahan ajar bentuk-bentuk geometri berbasis cerita menggunakan kombinasi model pengembangan Rowntree dan evaluasi Tessmer. Model pengembangan Rowntree terdiri dari tiga tahap yaitu tahap perencanaan, tahap pengembangan dan tahap evaluasi. Di tahap evaluasi menggunakan Tesmmerr kegiatan yang dilakukan terdiri dari 4 tahap yaitu self evaluation, expert review, one-to-one evaluation dan small group evaluation karena hanya menguji kevalidan dan melihat kepraktisan produk. Teknik pengumpulan data  menggunakan wawancara, walkthrough dan observasi. Hasil expert review nilai rata-rata yang diperoleh dari validasi materi dan desain sebesar 3,65 yang dikategorikan sangat valid. Kategori valid disini berarti sudah dikembangkan dengan teori yang memadai, difokuskan pada aspek isi, format perangkat pembelajaran memadai memadai pada desain tampilan, kemasan, penggunaan font dan komponen produk antara satu dengan yang lainnya berhubungan secara konsisten. Selanjutnya tahap one to one evaluation didapatkan rata-rata hasil observasi anak sebesar 88,86% dengan kategori sangat praktis dan tahap small group evaluation didapatkan hasil rata-rata observasi sebesar 91,66% kategori sangat praktis. Kategori paktis disini berarti bahan ajar yang dikembangkan mudah digunakan oleh pengguna baik guru maupun anak, mudah pengadministrasiannya seperti mudah dalam pelaksanaan pemberian penilaian dengan petunjuk yang jelas. Berdasarkan semua tahap yang telah dilakukan maka dapat disimpulkan bahwa bahan ajar bentuk-bentuk geometri berbasis cerita dinyatakan valid dan praktis. Kata Kunci: pengembangan bahan ajar, bentuk-bentuk geometri berbasis cerit, anak usia 5-6 tahun
Detection and Identification of Polymorphism in Mutant Strawberry (Fragaria spp.) Plants Based on Cleaved Amplified Polymorphic Sequences Molecular Markers Aristya, Ganies Riza; Ayundai, Melin; Putri, Fauzana; Widiastuti, Ani; Kasiamdari, Rina Sri
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 8, No 1 (2020)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2020.109.15-20

Abstract

Strawberry (Fragaria spp.) has a high economic value and various benefits, but the production of strawberry plants in Indonesia is still low in terms of both quantity and quality. Strawberry plant breeding can be done in various techniques, one of which is polyploidization. Polyploidization by an induction of colchicine at various concentrations in strawberry crops cv. California and Festival resulted in superior phenotype characteristics. To prove the existence of a change in ploidy in strawberry, then research at the molecular level needs to be done. The purposes of this study were to find out changes in ploidy of strawberry plants using CAPS molecular markers and to detect the polymorphism in strawberry plants quickly. The samples used were young leaves. Main procedure was the cutting of the amplified DNA using restriction enzymes of TaqI and HaeIII. The results showed that CAPS molecular markers were capable of detecting polymorphism quickly and efficiently in strawberry plants. Specific bands among strawberry plants having undergone polyploidization and those not having undergone polyploidization can be seen on the differences in monomorphic or polymorphic bands between the control plants and treated plants.Â