Purwanto Agustiono, Purwanto
Universitas Gadjah Mada Yogyakarta

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THE INFLUENCE OF Cu CONCENTRATION ON Cu-ZEOLITE TO ANTIBACTERIAL POWER IN STREPTOCOCCUS MUTANS Irnawati, Dyah; Agustiono, Purwanto; Wardhani, Endi Hanifah
Jurnal Zeolit Indonesia Vol 9, No 2 (2010)
Publisher : Jurnal Zeolit Indonesia

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Abstract

Microorganisms on the mouth can cause disease on hard and soft muscular of the mouth. One of the microorganisms is a bacteria S. mutans which become etiology of dental caries. The copper (Cu) is one of chemical agents to control microorganism growth. The copper could be combined with zeolite as antibacterial materials. This study aimed to known the affect Cu concentration on Cu-Natural zeolite to antibacterial power of S. mutans. The study was conducted by making five groups of Cu-Zeolite are 0,05M, 0,1M, 0,15M, 0,2M, and 0,25M. 100 mesh zeolite powder was activated at temperature 200 oC during one hours. CuCl2 solution with concentration of 0,05 M until 0,25 M are made each with volume of 80 mL. Cu-zeolite made by reacting 2 gram of zeolite powder with 80 ml of CuCl2 solution for one hours at temperature of 100 oC, then filtered, washed, and dried on the oven at 100 oC during 24 hours. S. mutans bacterial (0,1 mL x 108 CFU/mL) were grown in solid media MHA, then Cu Zeolite (30 mg) was included on pitting at gel media an incubated for 24 hours at temperature 37 oC (n=5). Transulent zone diameter was measured with digital slide-term (0.01 mm). The data were analyzed by anava one stripe and HSH with level signification of 0,05. The result showed an average of translucent zones was 0 mm (0,05 M gropus), 16,03 + 0,47 mm (0,10M), 16,45 + 0,91 mm (0,15M), 18,08 + 0,39 mm (0,20M), and 18,26 + 0,68 mm (0,25M). Anava test showed Cu concentration on 0,10 M up to 0,25 M of Cu Natural zeolite was significantly effect on translucent zones of S. mutans (p< 0,01). The result of HSD showed a significantly difference among all gropus, except on 0,01 M and O,15 M, and 0,20 m and 0,25 m (p>0,05). Conclusion of this study is Cu concentration in Cu-natural zeolite has affected antibacterial power of S. mutans. 0,01 M of Cuconcentration in Cu-natural zeolite has an antibacterial power to S. mutans and highest antibacterial power was on concentration groups of 0,20M.
The Effect Of Arrowroot Starch (Marantha Arundiceae L) Into Alginate Towards Alginate Impression Setting Time Yustri Anita, Lelly; Agustiono, Purwanto
Insisiva Dental Journal Vol 1, No 2 (2012)
Publisher : Insisiva Dental Journal

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Abstract

The demand of alginate increase overtime, in otherhand, Indonesia could not produce its own alginate yet. Limited access to get alginate in certain region of Indonesia raise an idea to apllicate arrowroot starch into alginate to reduce alginate using. One of impression material requirment is has suitable setting time. The effect of adding arrowroot starch into alginate towards its dimensional stabillity has been published, but the effect toward its setting time has not been evaluated. Methods : This study using 60 sample that divided into 4 groups (control, 45%, 50%, and 55% of added arrowroot starch). Acrilic stick is used by entering it into alginate after 10 second, and replied until no alginate on acrilic stick. The time when no alginate on acrilic stick was record by stopwatch. Result : Statistically, KruskallWallis test is show that arrowroot starch which add into alginate have significant effect towards alginate impression setting time with p=0,000. Conclusion : Arrowroot starch which has been added into alginate has an effect towards alginate impression setting time.
Pengaruh Lama Perendaman dalam Minuman Secang Terhadap Perubahan Warna Ionomer Kaca Atraumatic Testorative Treatment Wijaya, Gandha; Agustiono, Purwanto
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 8, No 1 (S) (2008): Januari
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v8i1 (S).9414

Abstract

Ionomer kaca ART merupakan bahan restorasi yang bersifat hidrofil. Ionomer kaca ART akan menycrap air secara perlahan-lahan dalam jangka waktu tertentu. Minuman secang didalamnya terkandung zat pewarna yang sifatnya lamt dalam air yaitu Brazilein. Penelitian ini bertujuan untuk mengetahui pengaruh lama perendaman dalam minuman secang terhadap perubahan warna pada ionomer kaca ART.Penelitian ini menggunakan desain penelitian ekperimental laboratoris dengan membenikan 3 kelompok perlakuan yang berbeda pada 5 sampel yang sama yaitu dengan dengan pemberian perlakuan perendaman selama 3 hari, 6 hari, dan 9 hari dalam minuman secang dengan sampel bahan restorasi ionomer kacaART ( GC Fuji IX 1-1 PKG). Perbedaan nilai kromatisitas Warnanya diuji dengan alat uji Spectrophotometer UV-2400 (PC) pada setiap perlakuan. Uj i analisis dengan menggunakanAnava Klasifikasi Tunggal dan uj i LSDQO5 .Hasil penelitian didapatkan bahwa nilai rata-rata chromatisitas perubahan Warna ionomer kaca ART terendah adalah pada kelompok 3 hari, kemudian diikuti kelompok perendaman 6 hari dan nilai rata-rata chromatis 1' tas perubahan Warna tertinggi pada kelompok 9 hari. Kemudian dianalisis dengan Anava 1 j alur didapatkan p 0,05 , bahwa terdapat pengamh lama perendaman dalam minuman secang terhadap pembahan warna ionomer kacaART. Setelah itu dilakukan uji LSD 0,05 menunjukkan adanya perbedaan nilai pembahan warna yang bermakna antar setiap kelompok lama perendaman, yaitu perubahan Warna lebih banyak telj adi pada kelompok lama perendaman 3 hari ke 6 hari dibandingkan 6 hari ke 9 hari.