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All Journal Jurnal Pengajaran MIPA Indonesian Journal of Biotechnology IJIET (International Journal of Indonesian Education and Teaching)
Tony Hadibarata, Tony
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Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Materials Science & Nanotechnology

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Decolorization of Remazol Briliant Blue R by Laccase from White Rot Fungus Polyporus sp. S133 Hadibarata, Tony; Tachibana, Sanro
Indonesian Journal of Biotechnology Vol 14, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.014 KB)

Abstract

The decolourization of the recalcitrant dye RBBR by the culture filtrate of Polyporus sp. S133 and its isolatedlaccase was investigated. The laccase alone decolorized RBBR. A small molecular weight redox mediator (HBT) wasnecessary to increase the decolorization. The purified laccase totally decolorized the dye of 200 mg l-1 initialconcentration of RBBR when only 1.5 U ml-1 of laccase was used in the reaction mixture. The effects of differentphysicochemical parameters were tested and optimal decolorization rates occurred at pH 5 and at a temperature of 50°C. The effect of surfactants on the decolourization of RBBR was tested with Tween 80, Tween 20, and Brij 35. It wasdemonstrated that Tween 80 was inhibiting substrate for the decolorization while Tween 80 and Brij 35 was noinhibiting effect for the decolorization. Provided that all of the condition is included, it is suggested that laccase maybe suitable for the wastewater treatment of similar anthraquinone dyes.Keywords: Decolorization; Laccase; Remazol Brilliant Blue R (RBBR); Polyporus sp. S133
Identification of Metabolic Intermediates in Microbial Degradation of Chrysene by Armillaria sp. F022 Hadibarata, Tony; Kristanti, Risky Ayu
Indonesian Journal of Biotechnology Vol 15, No 2 (2010)
Publisher : Universitas Gadjah Mada

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Abstract

To degrade chrysene, a polycyclic aromatic hydrocarbon (PAH), Armillaria sp. F022, a fungus collected from a soil, was used. Maximal degradation (77%) was obtained when Armillaria sp. F022 was incubated in cultures agitated at 120 rpm for 30 days, as compared to just 41% degradation in stationary culture. Furthermore, the degradation of chrysene was affected by the addition of surfactants. The mechanism of degradation was determined through identification of the intermediates. Several enzymes (manganese peroxidase, lignin peroxidase, laccase, 1,2-dioxygenase and 2,3-dioxygenase) produced by Armillaria sp. F022 were detected in the culture. The highest level of activity was shown by 1,2-dioxygenase after 20 days (143.6 U l-1). Theseligninolytic and dioxygenase enzymes played an important role in the oxidation of chrysene. Chrysene was indeed degraded by Armillaria sp. F022 through several intermediates, chrysenequinone, 2-((1E,3E)-4-carboxy-3-hydroxybuta-1,3-dien-1-yl)-1-naphthoic acid , 1-hydroxy-2-naphthoic acid, and gentisic acid.Keywords : Biodegradation, Chrysene, Metabolites, Armillaria sp. F022
ENGINEERING STUDENTS’ PERCEPTION ABOUT LEARNING AND ITS VARIOUS TYPES OF ASSESSMENT Hadibarata, Tony; Syafiuddin, Achmad
Jurnal Pengajaran MIPA Vol 23, No 1 (2018): JPMIPA: Volume 23, Issue 1, 2018
Publisher : Faculty of Mathematics and Science Education, Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18269/jpmipa.v23i1.8930

Abstract

Students’ perception of assessments can serve as a guiding light for improving overall educational practices. A questionnaire was collected from 80 engineering student to discover students' perceptions of different types of assessment and their overall perceptions of various type of assessments advantages in enhancing learning experiences. The majority of students strongly agreed that quiz and case study was beneficial and helpful for enhancing learning experience in engineering courses. On the contrary, project presentation, journal review, essay, interview, and demonstration was seemed unhelpful for students to enhance their learning experience. In terms of enjoyment, a discrepancy was found for what engineering students’ perceived as important assessment tools for learning and what they actually enjoy doing.
TEACHING GREEN ENGINEERING PRINCIPLES AND APPLICATION THROUGH ACTIVE LEARNING Hadibarata, Tony
International Journal of Indonesian Education and Teaching (IJIET) Vol 3, No 2 (2019): July 2019
Publisher : Sanata Dharma University Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (477.639 KB) | DOI: 10.24071/ijiet.v3i2.1746

Abstract

Active learning refers any approach to instruction in which all students are required to involve in the learning process. The purpose of the manuscript is to evaluate the application of active learning in teaching Green Engineering Principles and Applications as a compulsory course in environmental engineering department curriculum, Curtin University Malaysia. Green engineering can be defined as an approach of the design, process, product and commercialization that following environmentally conscious attitude, principles and values combined with multi-disciplinary engineering science that to minimize pollutant and promote local and global sustainability. Green engineering encompasses the conceptualization and implementation of reducing environmental impacts, maximize energy efficiency and develop the greener processes and product that bring environmental and economic benefit. A simple approach that combining the classical lecture-presentation and active engagement of the students with the course materials through case studies, problem solving and discussion has been developed. In conclusion, introducing the active learning to the students on solving any problems improve the students’ ability in achieving the course outcome and thus the programme outcome of the Department of Environmental Engineering, Curtin University Malaysia.DOI: doi.org/10.24071/ijiet.2019.030207
Co-Authors Risky Ayu Kristanti Sanro Tachibana Syafiuddin, Achmad