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All Journal Jurnal Ilmiah Farmasi JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Journal of Dedicators Community Jurnal Kesehatan Islam : Islamic Health Journal Journal of Pharmaceutical Care Anwar Medika Soeropati: Journal of Community Service Caring : Jurnal Pengabdian Masyarakat Jurnal Kedokteran Komunitas (Journal of Community Medicine) Jurnal Farmasi Sains dan Praktis
Anita Puspa Widiyana
Department Of Pharmacy, Faculty Of Medicine, Universitas Islam Malang, Malang, Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Validasi dari Spektrofotometri UV-Vis dan Kandungan Total Flavonoid Ekstrak Etanol dari Akar Alang-Alang (Imperata cylindrica) dan Daun Pegagan (Centella asiatica) Widiyana, Anita Puspa
Journal of Pharmaceutical Care Anwar Medika (J-PhAM) Vol 3 No 2 (2021): Journal of Pharmaceutical Care Anwar Medika
Publisher : STIKES Rumah Sakit Anwar Medika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36932/jpcam.v3i2.69

Abstract

Validasi sebagai kontrol kualitas kandungan senyawa kimia dari bahan alam. Salah satu kandungan kimia yaitu flavonoid yang banyak terdapat di dalam akar alang-alang dan daun pegagan. Penelitian ini bertujuan untuk menjamin metode analisis memenuhi persyaratan dan menentukan kadar flavonoid. Tahapan penelitian meliputi ekstraksi, validasi dan penetapan total flavonoid. Ekstraksi dilakuan dengan merendam simplisia kering dalam pelarut etanol p.a selama 3x24 jam. Pelarut diuapkan menggunakan rotary evaporator sampai terbentuk ekstrak kental. Pengujian validasi meliputi linieriatas, akurasi, presisi, LOD dan LOQ. Penetapan total flavonoid dilakukan dengan mengukur serapan pada panjang gelombang maksimum 428,2 nm. Hasil validasi meliputi nilai koefesien korelasi (R) sebesar 0,998, presisi % RSD <2 %, % akurasi 99,53-97,98%, LOD 3,02 ppm dan LOQ 9,15 ppm. Total flavonoid dari ekstrak etanol akar alang-alang sebesar 36,39 ± 0,08 mg/g QE dan daun pegagan sebesar 102,10 ± 0,08 mg/g QE. Kesimpulannya yaitu metode yang digunakan memenuhi syarat validasi dan kandungan total flavonoid ekstrak etanol daun pegagan lebih tinggi dibandingkaan akar alang-alang.
Design and Molecular Docking Studies of Quinazoline Derivatives as Antiproliferation Anita Puspa Widiyana; Galih Satrio Putra; Luthfi Ahmad Muchlashi; Mellany Ika Sulistyowaty; Tutuk Budiati
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 3 No. 2 (2016): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.943 KB) | DOI: 10.20473/jfiki.v3i22016.44-48

Abstract

Background: Nowadays, a lot of new active substances as anticancer agents have been developed. One of the protein targets of anticancer is selective cyclooxygenase-2 (COX-2). Selective COX-2 is the regulator of cell proliferation. Objective: In this research, quinazoline derivatives were used to design the anticancer agent through a selective COX-2 inhibition. The potential activity of quinazoline derivatives could be increased by substitution in position 2 and 3 of quinazolinone. Molecular docking of selective COX-2 inhibition was required to predict their antiproliferation activity. Methods: The molecular docking of quinazoline derivatives was carried out using Molegro Virtual Docker (MVD) Ver.5.5. Twenty-one of quinazoline derivatives were docked into selective COX-2 with PDB code 3LN1. The interaction was evaluated based on the re-ranked score comparison between quinazoline derivatives with co-crystallized ligand CEL_682. Celecoxib was used as the reference to this research. Results: The result indicated that 18 of 21 quinazoline derivatives showed the approximately re-ranked score -131.508 to -108.418 kcal/mol. Eight of these 18 new quinazoline derivatives have re-ranked score better than Celecoxib. Conclusions: In conclusion, 8 of the new quinazoline derivatives are feasible to be synthesize and performed their in vitro evaluation.
HEALTH EDUCATION FOR STUDENT CADRES IN MIDDLE SCHOOL HEALTH CENTER TO IMPROVE THEIR KNOWLEDGE OF ADDITIONAL AND BALANCE NUTRITION Bachtiar Rifai Pratita Ihsan; Valentina Yurina; Eva Putri Arfiani; Diah Royani Meisani; Luthfi Ahmad Muchlashi; Anita Puspa Widiyana
Caring : Jurnal Pengabdian Masyarakat Vol 1, No 2 (2021): CARING Jurnal Pengabdian Masyarakat (Agustus 2021)
Publisher : Caring : Jurnal Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (732.288 KB) | DOI: 10.21776/ub.caringjpm.2021.001.02.3

Abstract

Food additives are materials added to food to affect the nature or shape of the food. These condiments can cause adverse effects on health if being consumed every day more than the amount in mg/Kg body weight. In addition, there are food additives that are prohibited, such as boric acid, formalin, salicylic acid, and others. The lack of understanding of junior high school (SMP) students regarding food additives that are allowed and prohibited cause students to be more likely exposed to foods that contain harmful substances that will accumulate or cause negative effects in the long term. This Community Service Activity aims to improve the understanding of School Health Unit (UKS) cadre students regarding food additives and balanced nutrition. This activity was attended by students from SMPN 1 Singosari, SMP 4 Muhammadiyah Singosari, and SMP Islam Bani Hasyim Singosari. Before and after the counseling material regarding food additives and balanced nutrition, the level of understanding was measured using pre and post-tests. The test results were then analyzed using independent T-test statistics with a confidence level of 95.  The results of this activity indicate that there is an increase in participants' knowledge (40%) about food additives and balanced nutrition. Counseling is one of the ways to increase students' understanding of food additives as food safety and balanced nutrition is important to support growth and development.
Combination of ethanolic extract on total flavonoid Centella asiatica L. leaves and Imperata cylindrica L. roots with UV-VIS spectrophotometric method Anita Puspa Widiyana; Sri Herlina; Didi Nurhadi Illian
Jurnal Ilmiah Farmasi Vol. 18 No. 1 (2022): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol18.iss1.art6

Abstract

Background: Flavonoids are widely employed as phytomedicines and as secondary metabolites generated by plants, where they serve key roles in plant physiology. Antioxidant, anti-inflammatory, anticancer, antibacterial, antifungal, and antiviral activities are only a few of the potential biological effects of flavonoids. The discovery of medicinal plants containing flavonoid chemicals is potential as a supportive and preventative treatment, notably for COVID-19 which has caused a pandemic in several countries, including Indonesia.Objective: This study aimed to determine the total flavonoid content in the ethanolic extracts of C. asiatica leaves and Imperata cylindrica roots to find the potential of flavonoid-rich plants as an alternative source of COVID-19 treatment.Methods: The ethanolic extracts from the combination of C. asiatica leaves and I. cylindrica roots (with five combination ratios) were tested for total flavonoid content using the UV-Vis Spectrophotometry method. The total flavonoid content of the extract combination was analyzed using a one-way ANOVA test.Results: The total flavonoid contents of the combination of C. asiatica leaf and I. cylindrica root extracts at a ratio of 1:1, 2:3, 3:2, 4:1, and 1:4 were 45.88 ± 0.08, 42.14 ± 0.08, 40.52 ± 0.08, 66.28 ± 0.08, and 40.88 ± 0.13 mg/g EQ, respectively. The homogeneity with Levene's test obtained a p-value of 0.303. The one-way ANOVA exhibited the p-value of F-test statistics < 0.001. Conclusion: The total flavonoid contents of ethanolic extracts from C. asiatica leaves and I. cylindrica roots are at a ratio of 4:1 > 1:1 > 2:3 > 1:4 > 3:2. The high total flavonoid content plays a role in increasing anti-inflammatory and immunomodulatory activities in COVID-19 patients.Keywords: Total flavonoid content, C. asiatica, I. cylindrica, UV-Vis Spectrophotometry, COVID-19
STUDI PUSTAKA SISTEMATIS: PENGARUH POLIFENOL DELIMA (Punica granatum L.) TERHADAP KADAR PROSTAGLANDIN E2 DARI SEL MAKROFAG RAW264.7 Faizah Dwi Qurrotul Aini; Anita Puspa Widiyana; Doti Wahyuningsih
Jurnal Kedokteran Komunitas Vol 9, No 1 (2021)
Publisher : Jurnal Kedokteran Komunitas (Journal of Community Medicine)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (517.692 KB)

Abstract

Abstrak: Prostaglandin E2 (PGE2) adalah senyawa yang akan meningkat pada jaringan inflamasi dan berkontribusi memodulasi nyeri. Delima (Punica granatum L.) dilaporkan memiliki potensi anti-inflamasi dan anti-nyeri neurogenik. Studi pustaka sistematis (SPS) ini mempelajari potensi anti-inflamasi ekstrak dan senyawa polifenol delima terhadap penurunan kadar PGE2 pada sel makrofag RAW264.7. Metode: Studi pustaka sistematis. Data penelitian dikumpulkan dari PubMed Central, PubMed, dan Google Scholar berdasarkan kata kunci pomegranate atau Punica granatum, inflammation, PGE2, dan sel makrofag RAW264.7. Delapan artikel memenuhi kriteria inklusi dan ditetapkan untuk ditelaah. Hasil: Ekstrak kulit dan bunga delima serta senyawa polifenol dalam buah dan kulit delima terbukti mampu menurunkan kadar PGE2 pada sel makrofag RAW264.7. Aktivitas anti-inflamasi ekstrak kulit dan bunga delima serta senyawa polifenol dalam buah dan kulit delima menurunkan kadar PGE2 melalui hambatan ekspresi Toll-Like Receptor 4 (TLR4), hambatan aktivasi jalur pensinyalan Mitogen-Activated Protein Kinase (MAPK), hambatan aktivasi faktor transkripsi Nuclear Factor-Kappa B (NF-κB), serta hambatan ekspresi dan regulasi enzim COX-2. Data menunjukkan bahwa senyawa polifenol delima menghambat sintesis PGE2 secara dependen. Kesimpulan: Senyawa polifenol delima mampu menurunkan kadar PGE2 pada sel makrofag RAW264.7.Kata kunci: Delima, Inflamasi, PGE2, Sel Makrofag RAW264.7
STUDI IN SILICO AFINITAS SENYAWA AKTIF BIJI KACANG MERAH (Phaseolus vulgaris) TERHADAP PROTEIN SIRTUIN-1 (SIRT-1) DAN NUCLEAR RELEATING FACTOR-2 (NRF2) UNTUK MENCEGAH ALZHEIMER M Josie Yusuf; Anita Puspa Widiyana; Dini Sri Damayanti
Jurnal Kedokteran Komunitas Vol 10, No 2 (2022)
Publisher : Jurnal Kedokteran Komunitas (Journal of Community Medicine)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (412.845 KB)

Abstract

ABSTRAK Pendahuluan: Protein Sirtuin-1 (SIRT-1) dan Nuclear Releating Factor-2 (NRF2) berperan dalam mekanisme terjadinya Alzheimer. Secara empiris kacang merah (Phaseolus vulgaris) mengandung senyawa aktif untuk menghambat Alzheimer, namun mekanismenya terhadap SIRT-1 dan NRF2 belum pernah diteliti. Penelitian dilakukan untuk mengetahui mekanisme kacang merah mencegah Alzheimer melalui aktivasi SIRT-1 dan NRF2.Metode: Penelitian dilakukan secara In Silico dengan menggunakan docking server untuk menilai afinitas senyawa aktif biji kacang merah terhadap protein SIRT-1 dan NRF2. Uji farmakokinetik, fisikokimia dinilai dengan pkCSM (Predicting Small-Molecule Pharmacokinetic and Toxicity Properties). Analisa dilakukan dengan diskriptif analitik dengan melihat hasil afinitas senyawa aktif terhadap SIRT-1 dan NRF2 dibandingkan obat kontrol.Hasil: Didapatkan 5 senyawa aktif (beta sitosterol, sianidin, glisitein, biokanin A, dan genistein) dari 8 senyawa aktif yang diteliti memiliki afinitas tinggi terhadap protein SIRT-1 dan 5 Senyawa aktif (beta sitosterol, biokanin A, genistein, glisitein, dan formononetin) dari 20 senyawa aktif yang didockingkan memiliki afinitas yang tinggi terhadap protein NRF2. Hasil prediksi parameter fisikokimia seluruh senyawa aktif memenuhi hukum 5 Lipinski. Hasil Prediksi sifat farmakokinetik β-sitosterol adalah senyawa yang memiliki nilai paling baik.Kesimpulan: senyawa aktif biji kacang merah mempunyai potensi sebagai pencegahan alzheimer dengan mengaktifkan SIRT-1 dan NRF2 yaitu beta sitosterol. Beta sitosterol juga mempunyai afinitas, fisikokimia, farmakokinetik yg baik dan tidak bersifat toksik sehingga dapat menjadi kandidat kuat obat pencegahan Alzheimer melalui peningkatan SIRT-1 dan NRF2.Kata Kunci : Alzheimer; Phaseolus vulgaris; in silico
Perbandingan Kadar Merkuri (Hg) dan Aktivitas Antioksidan Ekstrak Metanolik Akar Eceng Gondok (Eichornia crassipes ) di Daerah Lawang dan Pasuruan Chasan Arfisa; Anita Puspa Widiyana; Yoni Rina Bintari
Jurnal Kedokteran Komunitas Vol 8, No 1 (2020)
Publisher : Jurnal Kedokteran Komunitas (Journal of Community Medicine)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.042 KB)

Abstract

Background: Water hyacinth (Eichornia crassipes) is an aquatic plant that grows on the surface of the water and is also a high accumulator of heavy metals. E. crassipes has compounds containing sulfihydryl group which can bind to Hg. E. crassipes in Lawang and Pasuruan regions is thought to have high Hg levels which may result in the high antioxidant activity. The purpose of this research was to determine the levels of mercury and antioxidant activity in the methanol extract of water hyacinth roots in Lawang and Pasuruan. Methods: This research is an experimental research in the laboratory using water hyacinth roots in Lawang and Pasuruan areas taken by cluster sampling area. The extraction was by maceration method for 72 hours with methanol solvent. Extraction is done in 2 ways, namely using a shaker and without a shaker. Hg test was measured using atomic absorption spectrophotometry (AAS) at a maximum wavelength of 253.96 nm.. The antioxidant activity test was using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and their result was check using by Uv-Vis  spectrophotometry (517 nm wavelength).Result: Hg content of the water hyacinth root extract Lawang shaker area 0.5287 ppm while without shaker 0.2207 ppm. For the Pasuruan shaker area 0.7910 ppm while without the shaker 0.7874 ppm (p <0.05). The antioxidant activity of the water hyacinth root extract of Pasuruan area was higher than that of Lawang area (p <0.05). IC50 value of Lawang samples with 334.8 ppm shakers; without shakers 205.4 ppm while Pasuruan samples with 161.94 ppm shakers; without a shaker 168.45 ppmConclusion: Macerated extraction of water hyacinth roots to Hg levels in Pasuruan area was higher than Lawang area. There is a relation between Hg levels and high antioxidant activity. The antioxidant activity of the water hyacinth root in Pasuruan area is higher than Lawang area.Keyword: Antioxidant, Water hyacinth, Lawang Malang. Pasuruan, Mercury (Hg)
COMPUTATION DESIGN OF QUINAZOLINE-4(3H)-ON DERIVATIVES AS CYCLOOXYGENASE-2 (COX-2) INHIBITOR Anita Puspa Widiyana
Jurnal Farmasi Sains dan Praktis Vol 7 No 2 (2021)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v7i2.4827

Abstract

The 3-(benzylideneamino)-2-(2,4-dichlorophenyl)-quinazoline-4(3H)-ones (BDCQ) are compounds developed as anticancer drugs and quinazolines. The activity and bioavailability of BDCQ derivatives as anticancer compounds that inhibit COX-2 can be predicted by computer programs and online servers. Substituents are added at positions 2 and 3 to the quinazoline-4(3H)-on ring, such as -H, -NO2, -OCH3, -N(CH3)2, -SO2NH2, -OH, and –OCH3. QSAR as COX-2 inhibitor analysis was performed by SPSS Ver. 21 software. Lipinski’s rule of five for determining bioavailability is performed by an online server at http://ilab.acdlabs.com. The best QSAR equation used to predict the COX-2 inhibitors from these compounds is RS-pred = 0.372 Log P + 0.014 MR + 0.979 Etot – 4.859, with n= 12, R = 0.998; SE = 0.356, F = 805.252 and sig = 0.001. Six compounds were predicted to have good oral bioavailability, such as 3-(benzylideneamino)-2-(2,4-dichlorophenyl)quinazoline-4(3H)-one, 2-(2,4-dichlorophenyl)-3-((2-nitrobenzylidene)amino)quinazoline-4(3H)-one, 2-(2,4-dichlorophenyl)-3-((3-nitrobenzylidene)amino)quinazoline-4(3H)-one, 2-(2,4-dichlorophenyl)-3-((2-methoxybenzilidene)amino)quinazoline-4(3H)-one, 2-(2,4-dichlorophenyl)-3-((3-methoxybenzylidene)amino)quinazolin-4(3H)-one, and 2-(((2-(2,4-dichlorophenyl)-4-oxoquinazolin-3(4H)-yl)imino)methyl)- benzenesulfonamide. This research can be used as an in vitro and in vivo study for BDCQ derivatives as anticancer drugs.
PHYTOCHEMICAL ANALYSIS AND TOTAL FLAVONOID CONTENT ON ETHANOL AND ETHYL ACETATE EXTRACT FROM NEEM (AZADIRACHTA INDICA JUSS.) LEAVES UTILIZING UV–VIS SPECTROPHOTOMETRIC Anita Puspa Widiyana; Didi Nurhadi Illian
Jurnal Farmasi Sains dan Praktis Vol 8 No 1 (January-April 2022)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v8i1.6582

Abstract

The plants are rich sources of secondary metabolites, a bioactive compound that has various activities. Flavonoids, as a type of secondary metabolite, have been reported to possess anticancer, antioxidant, antibacterial, anti-inflammatory, and antiviral activities. Flavonoid has been found abundantly in Neem (Azadirachta indica Juss.) leaves. The difference in total flavonoid content might be an occurrence of the different solvent types and concentrations. The present study was conducted to analyze the phytochemical and determine the total flavonoid content of neem leaves extract using two different solvents (namely 70% ethanol and ethyl acetate) using UV-Vis spectrophotometric. The extraction from neem leaves was performed by maceration method. Phytochemical analysis of neem leaves reveals several secondary metabolites: flavonoids, steroids/triterpenoids, tannins, and saponins. Total flavonoid content from both extracts was determined by utilizing the UV-Vis spectrophotometric method at a maximum wavelength of 428.2 nm with three repetitions, and also quercetin was used as a standard. Total flavonoid content from neem leaves extract in solvents of 70% ethanol and ethyl acetate had a value of 118.57 ± 0.08 mg/g QE and 74.17 ± 0.20 mg/g QE, respectively. Neem leaves extract in solvents of 70% ethanol and ethyl acetate had identical phytochemical content. Total flavonoid content of neem leaves from 70% ethanol extract was higher than ethyl acetate extract.
STUDI IN SILICO AFINITAS SENYAWA AKTIF BIJI KACANG MERAH (Phaseolus vulgaris) TERHADAP PROTEIN SIRTUIN-1 (SIRT-1) DAN NUCLEAR RELEATING FACTOR-2 (NRF2) UNTUK MENCEGAH ALZHEIMER M Josie Yusuf; Anita Puspa Widiyana; Dini Sri Damayanti
Jurnal Kedokteran Komunitas Vol 10 No 2 (2022)
Publisher : Jurnal Kedokteran Komunitas (Journal of Community Medicine)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK Pendahuluan: Protein Sirtuin-1 (SIRT-1) dan Nuclear Releating Factor-2 (NRF2) berperan dalam mekanisme terjadinya Alzheimer. Secara empiris kacang merah (Phaseolus vulgaris) mengandung senyawa aktif untuk menghambat Alzheimer, namun mekanismenya terhadap SIRT-1 dan NRF2 belum pernah diteliti. Penelitian dilakukan untuk mengetahui mekanisme kacang merah mencegah Alzheimer melalui aktivasi SIRT-1 dan NRF2.Metode: Penelitian dilakukan secara In Silico dengan menggunakan docking server untuk menilai afinitas senyawa aktif biji kacang merah terhadap protein SIRT-1 dan NRF2. Uji farmakokinetik, fisikokimia dinilai dengan pkCSM (Predicting Small-Molecule Pharmacokinetic and Toxicity Properties). Analisa dilakukan dengan diskriptif analitik dengan melihat hasil afinitas senyawa aktif terhadap SIRT-1 dan NRF2 dibandingkan obat kontrol.Hasil: Didapatkan 5 senyawa aktif (beta sitosterol, sianidin, glisitein, biokanin A, dan genistein) dari 8 senyawa aktif yang diteliti memiliki afinitas tinggi terhadap protein SIRT-1 dan 5 Senyawa aktif (beta sitosterol, biokanin A, genistein, glisitein, dan formononetin) dari 20 senyawa aktif yang didockingkan memiliki afinitas yang tinggi terhadap protein NRF2. Hasil prediksi parameter fisikokimia seluruh senyawa aktif memenuhi hukum 5 Lipinski. Hasil Prediksi sifat farmakokinetik β-sitosterol adalah senyawa yang memiliki nilai paling baik.Kesimpulan: senyawa aktif biji kacang merah mempunyai potensi sebagai pencegahan alzheimer dengan mengaktifkan SIRT-1 dan NRF2 yaitu beta sitosterol. Beta sitosterol juga mempunyai afinitas, fisikokimia, farmakokinetik yg baik dan tidak bersifat toksik sehingga dapat menjadi kandidat kuat obat pencegahan Alzheimer melalui peningkatan SIRT-1 dan NRF2.Kata Kunci : Alzheimer; Phaseolus vulgaris; in silico
Co-Authors Bachtiar Rifai Pratita Ihsan Chasan Arfisa Damayanti, Dini Sri Diah Royani Meisani Didi Nurhadi Illian Didi Nurhadi Illian Doti Wahyuningsih Erna Sulistyowati Eva Putri Arfiani Faizah Dwi Qurrotul Aini Galih Satrio Putra Husain Latuconsina Intan Nurkholidah Luthfi Ahmad Muchlashi Luthfi Ahmad Muchlashi M Josie Yusuf Majida Ramadhan Majida Ramadhan Mellany Ika Sulistyowaty Muhammad Ali Rahmatullah Muhammad Fadlan Rofiq Muhammad Fahmi Hidayatullah Nour Athiroh Abdoes Sjakoer Sri Herlina Sri Herlina Tutuk Budiati VALENTINA YURINA Yoni Rina Bintari Yudi Purnomo