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All Journal Jurnal Kedokteran Komunitas (Journal of Community Medicine)
Nurma Alifia Rakhma
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Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Public Health

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Efek Penambahan Fraksi Semi Polar (F15-F19) Ekstrak Metanol Tapak Liman Pada Daya Hambat Amoksisilin dan Kloramfenikol Terhadap Bakteri Staphylococcus aureus dan Escherichia coli Nurma Alifia Rakhma; Zainul Fadli; Rio Risandiansyah
Jurnal Kedokteran Komunitas Vol 8, No 1 (2020)
Publisher : Jurnal Kedokteran Komunitas (Journal of Community Medicine)

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Abstract

Introduction: Elephantopus scabr linn contains various compounds which have antibacterial activity. The addition of antibiotic with herbs may increase antibiotic activity. However previous studies on Elephantopus scabr linn  was in the form of crude extract. Therefore, this study  separates compounds these into Semi polar fraction to determine the antibiotic activity and what compounds were contained therein.Methods: Methanol extract was fractionated using silica gel stationary phase and mobile phase 75 ml of ethyl acetate: 25 ml of  methanol. Phytochemical test were carried out with dragendorff spray, FeCl3 and Formaldehyde on Thin layer Chromatography. The interaction between herbs and antibiotics was assessed based on the AZDAST method and the inhibitory assessment using the Kirby bauer method.Result: Five fractions (f15-f19) were obtained using fractionation. Fraction 15 contain alkaloids, and fraction 16-19 contains alkaloids and phenol. On a single test, fraction 16 had clear zones against S.aureus with a value  6,66 ± 0,57 mm. In the combination test, fraction 18 and 19  had an antagonistic interaction with amoxicillin in S.aureus. Combination of fractions 16-19 had antagonistic interaction with chloramphenicol in S.aureus.Conclusion: Fraction 16 of Elephantopus Scabr Linn metanolic extract  was sinergistic with amoxicillin against S.aureus.Keywords: Semi Polar Fraction, Elephantopus scabr linn, S. aureus, E.coli
Co-Authors Muhammad Zainul Fadli Risandiansyah, Rio