This research aims to determine: The effect of organizational culture on the motivation of employees, Effect of effective communication on the motivation of employees, The influence of organizational culture on the performance, Effect of effective communication to employee performance, The effect of motivation on employee performance, The role of motivation in mediating effect of organizational culture on employee performance, The role of motivation in mediating the effects of effective communication to employee performance at the Parliament Secretariat Southeast Sulawesi Province. The design of this research is associative (causal). The object of this study is the performance of the staff of the Parliament Secretariat Southeast Sulawesi Province. The population in this study are all servants of Parliament Secretariat of Southeast Sulawesi province, amounting to 148 people. The samples using the formula Slovin. Analysis of data used in this research is descriptive statistical analysis and Partial Least Square (PLS). The results of this research concluded that: Cultural organizations significant positive effect on the motivation of employees, Cultural organizations significant positive influence on employee performance, Effective communication significant positive effect on employee motivation, Effective Communication siginifkan positive effect on employee performance, Motivation significant positive effect on the performance of employees, Motivation mediates the influence of organizational culture on employee performance, The motivation mediates the effect of effective communication to employee performance. This means that the motivation could be a mediating variable in strengthening effective communication influence on employee performance in Southeast Sulawesi Provincial Parliament Secretariat