Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist)

EFEK EKSTRAK BIJI KOPI ROBUSTA (coffeacanephora) TERHADAP pH SALIVA dan PERTUMBUHAN BAKTERI Staphylococcus aureus (ATCC® 29213™) (IN VITRO) Lubis, M Rizki Fauzi; Lindawati, Yumi
Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist) Vol 12 No 3 (2018): Jurnal Ilmiah PANNMED Periode Januari-April 2018
Publisher : Politeknik Kesehatan Kemetrian Kesehatan Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (359.981 KB)

Abstract

Kopi merupakan minuman yang digemari masyarakat. Kandungan dalam kopi terdiri dari kafein, trigonelin, sukrosa, monosakarida, asam klorogenat, dan asam nikotinat. Kafein pada kopi yang berguna sebagai obat antibakteri. Tujuan penelitian ini untuk mengetahui efek ekstrak kopi Robusta pada konsentrasi 100%, 50%, 25%, dan 12,5% untuk mempertahankan pH saliva normal dan mengetahui jumlah koloni Staphylococcus aureus. Besar sampel masing-masing perlakuan berjumlah 20 sampel untuk pengukuran pH saliva dan jumlah koloni Staphylococcus aureus. Pengumpulan saliva dilakukan pada pukul 9 sampai pukul 10 pagi. Pengukuran pH saliva menggunakan indikator pH saliva Hanna Instrument, dan pengujian bakteri Staphylococcus aureus (ATCC®29213™) menggunakan media Mannitol Salt Agar, selama 24 jam pada suhu 37oC, setelah itu dilakukan penghitungan jumlah koloni(CFU). Hasil uji T berpasangan menunjukkan ekstrak kopi Robusta konsentrasi 100%, 50%, dan 25% dapat menurunkan pH saliva secara signifikan. Pada uji kruskal wallis menunjukkan hasil yang signifikan antara seluruh konsentrasi ekstrak kopi Robusta. Hasil uji Mann Whitney U perbedaan jumlah koloni bakteri Staphylococcus aureus dari tiap konsentrasi ekstrak kopi Robusta adalah signifikan p <0,05. Uji Korelasi pearson antara pH kopi dan jumlah koloni bakteri Staphylococcus aureus pada tiap konsentrasi terdapat hubungan yang signifikan antara pH kopi terhadap jumlah koloni bakteri Staphylococcus aureus. Kesimpulan dari penelitian adalah ekstrak kopi Robusta secara signifikan dapat menurunkan pH saliva dan menurunkan jumlah koloni Staphylococcus aureus.
PERBANDINGAN NILAI KEKERASAN PERMUKAAN ENAMEL PADA PERENDAMAN OBAT KUMUR CENGKEH DENGAN DAN TANPA PENAMBAHAN SALIVA BUATAN Lindawati, Yumi; Novia, Novia
Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist) Vol 10 No 3 (2016): Jurnal Ilmiah PANNMED Periode Januari - April 2016
Publisher : Politeknik Kesehatan Kemetrian Kesehatan Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.417 KB)

Abstract

Nowadays herbal mouthwash is widely consumed by the society, one of which is mouthwash containing cloves. Cloves contain minerals such as calcium and phosphorus which can help the remineralization of enamel. Mouthwash containing clove oil has antibacterial, antimicrobial effects, and can inhibit decalcification of enamel. The purpose of this study was to investigate the effect of immersion in clove mouthwash and the effect of of immersion in clove mouthwash with addition of artificial saliva on the hardness of enamel surface after immersion in commercial orange juice. It was a laboratory experimental pre-posttest control group design. Thirty two permanent maxillary first premolar extracted for orthodontic reason which qualify inclusion and exclusion criteria were used in this study. All samples were immersed in commercial orange juice for 5 minutes for demineralization then they were divided into group of clove mouthwash immersion without addition of artificial saliva and group of clove mouthwash immersion with addition of artificial. The surface enamel hardness before immersion, after immersed in commercial orange juice and after immersed in clove mouthwash of each sample was measured using Microvickers Hardness Tester FM-800. The results of the research that has been tested with Paired T-Test showed a significant decrease (p<0,05) of surface enamel hardness in clove mouthwash immersion without addition of artificial saliva group, while the group of clove mouthwash immersion with addition of artificial saliva showed a significant increase (p<0,05) of surface enamel hardness. The conclusion of this research showed that the value of enamel hardness is higher with the addition of artificial saliva than without addition of artificial saliva in clove mouthwash.