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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Agrotrop : Journal on Agriculture Science Jurnal Pengendalian Hayati Plumula : Berkala Ilmiah Agroteknologi Proceeding International Conference on Global Resource Conservation Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) JURNAL AGROHITA Jurnal Ilmiah RESPATI Agrotechnology Research Journal Jurnal Pembelajaran dan Biologi Nukleus Agrienvi : Jurnal Ilmu Pertanian Nusantara Science and Technology Proceedings Berkala Penelitian Hayati AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment)

Arika Purnawati

Program Studi Agroteknologi Fakultas Pertanian UPN Veteran Jawa Timur

Author-ID : 5402990

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Other

Published : 24 Documents Claim Missing Document

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Endophytic Bacteria as Biocontrol Agents of Xanthomonas campestris pv. manihotis on Cassava In Vitro Purnawati, Arika
Proceeding International Conference on Global Resource Conservation Vol 4, No 1: Proceeding of 4th ICGRC 2013
Publisher : Proceeding International Conference on Global Resource Conservation

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.209 KB)

Xanthomonas campestris pv. manihotis was important pathogen on cassava can cause plant die but the control still had not been taken seriously but now was study about endophytic bacteria as biocontrol agents for it. Endophytic bacteria are microbes which live symbiotically inside the plant tissue and without causing disease symptoms and had potential as biocontrol agents for it. The purpose of this research is to know the potential of endophytic bacteria that was isolated from cassava as biocontrol agents for it. The potential ofÂ it was study using antagonism assay in vitro and characteristics of them based on the phenotypic characteristics were Gram reaction, hypersensitive reaction (HR) on tobacco leaf, anaerobic growth, catalase, carbohydrat hydrolyze, heat tolerance, NaCl tolerance. This assay used seed coating combination plate chlorofom method and used completely randomized design with 20 treatments ofÂ isolates and each was repeated three times. The result of this research were 20 endophytic bacteria isolates and 4 isolates can inhibit of Xanthomonas campestris pv. manihotis growth in vitro.Keywords: endophytic bacteria, Â biocontrol agents, Â Xanthomonas campestris Pv. manihotis, Â

Biodiversity of Endophytic Bacteria from Egg Plant in Lowland Purnawati, Arika; Nirwanto, Herry
Nusantara Science and Technology Proceedings 5th International Seminar of Research Month 2020
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.0934

Endophytic bacteria are a group of microbes that live in plant tissues without causing negative effects and don’t cause sick on plants, are found in various plants, and can be isolated from all parts of the plant: roots, stems, and seeds. Endophytic bacteria in nature, have high diversity because in a single plant can be found more than one kind of endophytic bacteria, so many plants found many kinds of endophytic bacteria. Endophytic bacteria that have been isolated and identified from eggplants. This study aimed to isolate endophytic bacteria from eggplants in lowland Kandat Kediri. Crop sampling was conducted using a systematic random method. The research stages are surveys and microbial isolation of endophytes. Isolation is done from the stems of a healthy eggplant around the sick eggplant. The crop samples were then washed with running water and cut 1-3 cm. The cutting was sterilized by ethanol 70% (30 sec), sodium hypochlorite 5% (5 min), ethanol 70% (30 sec) and rinsed with sterile water (3 minutes). The results are from the stem get 10 isolates of endophytic bacteria that have different characteristics.

Endophytic Bacteria as Biocontrol Agents of Tomato Bacterial Wilt Disease Purnawati, Arika
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

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Ralstonia solanacearum is one important pathogen on tomato because it caused wilt disease that can loss yield until 100%. The object of these experiment are to select of endophytic bacteria that was isolated from healthy tomato stems, root and to investigate of them as biocontrol agents for tomato bacterial wilt disease. There are 10 isolates successfully isolated and two isolates are Ps1 and Ps8 can inhibit of R. solanacearum. The result show the two isolates are Ps1 and Ps8 significantly suppress disease incidence until 8.07-9.19%.

UJI ANTAGONISME BAKTERI ENDOFIT ASAL TANAMAN PERTANIAN DATARAN RENDAH PADA MEDIUM AGAR Arika Purnawati; Noni Rahmadhini; Elly Syafriani
Plumula : Berkala Ilmiah Agroteknologi Vol 7 No 1 (2019): Plumula : Berkala Ilmiah Agroteknologi
Publisher : UPN VETERAN JAWA TIMUR

This research aims to obtain endophytic bacterial candidates from lowland agricultural plants that have potential as biological control agents for Ralstonia solanacearum on tomato plants. Bioassay was carried out using the soaking method of tomato seeds in the suspension of endophytic bacteria (108 cfu / ml) during 60 minutes then R. solanacearum was 24 hours old (108 cfu / ml) mixed with 6 ml of liquid agar 0.6% and poured onto the seed surface. Bioassays were observed at 24, 48, 72 and 96 hours. The study was conducted using Completely Randomized Design (CRD) with 10 endophytic bacterial isolates as single treatment factor and repeated 3 times. The results obtained showed that 10 endophytic bacterial isolates were able to inhibit the growth of R. solanacearum in agar medium, and the highest clear zone size was formed by H, I, J

UJI PATOGENESITAS BEBERAPA ISOLAT BAKTERI SIMBION NEMATODA ENTOMOPATOGEN TERHADAP LARVA KROP KUBIS Crocidolomia pavonana Aris Cahyono; Arika Purnawati; Tri Mudjoko; Presti Mardiyani
Plumula : Berkala Ilmiah Agroteknologi Vol 7 No 2 (2019): Plumula : Berkala Ilmiah Agroteknologi
Publisher : UPN VETERAN JAWA TIMUR

To control pest Crocidolomia pavonana in cabbage plants are still based on chemical and mechanical control. The methode is considered ineffective because excessive use of chemicals has a negative effect on the comodity and the environment. The application of plant protection technology using Biological Control Agents (BCA) is one of the solutions given. One of them is entomopathogenic nematode (EPN). The advantage possessed by EPN is the symbiosis with the symbiont bacteria that secretes toxins so that it can immobilize the host quickly. Based on this, a study was conducted to determine the pathogenicity of symbionic bacteria against Crocidolomia pavonana larvae, so that they could be developed as bioentomopathogenic in the Integrated Pest Control concept. The pathogenicity test on Crocidolomia pavonana larvae was carried out using 4 treatments placed in a Completely Randomized Design (CRD) with each treatment repeated 6 times. The results showed that in the three days of observation, the four bacterial isolates had the same ability to kill Crocidolomia pavonana larvae with the highest percentage of 100% and the lowest 88.9%. Thus the four isolates can be used as an alternative in controlling Crocidolomia pavonana larvae.

Potensi Rumput Laut Dalam Menghambat Pertumbuhan Jamur Aspergillus flavus KETUT SRIE MARHAENI JULYASIH; ARIKA PURNAWATI
Agrotrop : Journal on Agriculture Science Vol 9 No 1 (2019)
Publisher : Fakultas Pertanian Universitas Udayana

Potential of Seaweed in the Inhibition of Fungi Growth Aspergillus flavus. This use of synthetic chemicals as a controller of fungal growth in foodstuffs can have adverse effects on health, so it needs natural control ingredients that do not have an impact on human health. Various types of fungi such as Aspergillus flavus attack a lot of food items after harvest, these fungi can produce aflatoxin which is very toxic to consumers. Aflatoxin cannot be neutralized through cooking so efforts to avoid fungal contamination need to be done. One of the natural fungi controllers is by utilizing natural resources, including seaweed. Development of seaweed as an antifungal is an alternative that needs to be realized. For this reason, it is necessary to examine the potential inhibitory of seaweed A. flavus development. This study examined six types of seaweed like Gracilaria verrucosa, Euchema spinosum, Ulva lactuca, Ulva reticulata, Euchema edule, and Padina sp. The results showed that Padina sp. seaweed has a very strong potential inhibitory compared to other types of seaweed.

Kemampuan Antagonistik Beberapa Isolat Pseudomonad Fluoresen Terhadap Bakteri Ralstonia Solanacearum Penyebab Penyakit Layu Pada Tanaman Tomat Yenny WURYANDARI; Arika PURNAWATI; Triwidodo ARWIYANTO; Bambang HADISUTRISNO
Jurnal Pengendalian Hayati Vol 1 No 1 (2008)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.295 KB)

Bacterial Wilt disease (Ralstonia solanacearum) is a main problem in tomato plant.Controls of it have been optimum success yet. Control of it using certainly pseudomonad fluorescentstrain can pressure plant disease developments which cause soil pathogen.The aim of the research, getpseudomonad fluorescent from tomato rizosfer which can inhibit of wilt bacteria disease Ralstoniasolanacearum. Research methods are isolation and identification of pathogen bacteria andantagonistic bacteria. Biological control agents candidate selection was done with antagonistic invitro and inhibit mechanism test. The Research show; there is wilt symptom in tomato areal at WajakMalang. From isolation get Ralstonia solanacearum bacteria with identity white coloni, fluidal,irregular shape in YPGA media and high virulensi. From tomato rizosfer soil isolation in the sameareal get 130 isolate of pseudomonad fluorescent in King’s B media. Antagonistec test in vitro to itisn’t of all bacteria can inhibit Ralstonia solanacearum growing. From them which was tested, getvariation of inhibit zona from 4 mm until more than 30 mm. More of inhibit mechanism isbacteriostatic and only many is bacterisida.

VIABILITAS PSEUDOMONAD Fluorescens ISOLAT Pf-122 DALAM BEBERAPA FORMULA Yenny Wuryandari; Arika Purnawati; Siswanto Siswanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 2 (2009): June 2009
Publisher : The East Java Biological Society

The objectives of this research were to examine effect of several formulas on the viability of Pseudomonas Fluorescens isolate Pf- 122. The eight formulations include carriers, additives, and concentration of Pf-122. The efficacy of various formulation in maintaining the population of Pf-122 in storage was assessed. The result of the viability test of Pf-122 in formula showed that the highest population of Pf-122 in week 0 was observed on the formula 1, viz the formula with combination of pupuk kandang as carrier and CMC as additive and Pf-122 at the concentration of 1010 CFU/ml. The bacterial population of the formula number1 could reach 4.8 × 1010 CFU/formula and followed by the formulas number 2,3,4,5,6, and 7. The lowest population was observed on the formula number 8 with the density of 5.8 × 106 CFU/formula. The bacterial population decreased during storage in all of the formulas and even it could not be detectedin the formulas number 3 and 4 after the first week of storage. In the formulas number 1 and 2 survived within 3 months.

POTENSI KONSORSIUM Bacillus spp. DAN Pseudomonas fluorescens UNTUK MENGENDALIKAN PENYAKIT LAYU FUSARIUM PADA TANAMAN CABAI RAWIT Nensi Agustina; Arika Purnawati; Endang Triwahyu Prasetyawati
Plumula : Berkala Ilmiah Agroteknologi Vol 10 No 1 (2022): Plumula : Berkala Ilmiah Agroteknologi
Publisher : UPN VETERAN JAWA TIMUR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/plumula.v10i1.64

Cayenne pepper (Capsicum frutescens L.) is one of the horticultural crops with high economic value. The production of cayenne pepper is often constrained by stem wilt disease caused by the fungus Fusarium sp. This research aims to know the effect of the consortium of Bacillus spp. and Pseudomonas fluorescens as biological agents to control fusarium wilt in cayenne pepper. This research was conducted at the screenhouse of UPN "Veteran" East Java. The research began in January 2021 until April 2021. The study was arranged using a Split Plot Design with two factors, namely antagonistic bacteria and application time and was repeated 4 times. The first factor is antagonistic bacteria with 5 levels of treatment, namely without the provision of antagonistic bacteria, Bacillus spp. isolate code Ba9, Bacillus spp. isolate code Ba17, P. fluorescens, Bacillus spp. isolate code Ba9 + P. fluorescens, Bacillus spp. isolate code Ba17 + P. fluorescens. The second factor is the application time with 2 levels of treatment, namely before transplanting seedlings and after transplanting seedlings. If it is known that there is a significant effect of the treatment, then the Duncan Multiple Range Test (DMRT) is carried out at the 5% level. The treatment of the consortium of Bacillus spp. isolate code Ba9 and P. fluorescens caused a delay in the incubation period of Fusarium sp. by 23.42%, increasing plant height by 20.55%, and accelerating the emergence of prospective flowers, while the treatment with the consortium of Bacillus spp. isolate code Ba17 and P. fluorescens suppressed the intensity of fusarium wilt disease by 29.03%, increasing the number of leaves by 56.58% compared to the single aplication of bacteria.

Deteksi Cendawan Terbawa Benih Gandum asal Australia Menggunakan Metode Blotter Test Indah Pemuda; Arika Purnawati; Tri Mujoko
AGRITROP Vol 20, No 1 (2022): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agritrop.v20i1.6975

Gandum merupakan salah satu komoditas yang diimpor Indonesia untuk memenuhi kebutuhan pasar nasional. Indonesia mengimpor gandum pada 2019 sebanyak 10,629 juta ton dan pada 2020 sebanyak 10,229 juta ton. Benih gandum yang diimpor berpotensi sebagai pembawa patogen terutama cendawan yang bersifat OPTK golongan A1 sehingga penting untuk dilakukan deteksi awal patogen untuk mencegah penyebarannya di Indonesia. Penelitian ini bertujuan untuk mendeteksi cendawan terbawa benih gandum asal Australia (Australia I dan Australia II) menggunakan metode blotter test. Tahapan metode ini sebagai berikut penyiapan sampel benih gandum asal Australia I dan Australia II, deteksi cendawan benih menggunakan metode blotter test, dan identifikasi cendawan secara makroskopis dan mikroskopis. Keanekaragaman cendawan terbawa benih gandum dianalisis secara deskriptif berdasarkan karakter makroskopis maupun mikroskopis. Hasil pengamatan ditemukan 8 spesies cendawan terbawa benih gandum asal Australia (Australia I dan Australia II), yaitu Alternaria alternata, Alternaria brassicicola, Alternaria japonica yoshi, Aspergillus flavus, Aspergillus niger, Bipolaris cyonodontis, Penicillium sp., dan Rhizopus stolonifer. Keanekaragaman cendawan yang ditemukan pada sampel Australia I lebih beragam dibandingkan Australia II.

Co-Authors Alvia Cindy Apriliani Anisa Rizki Amalia Aris Cahyono Bambang HADISUTRISNO Elly Syafriani Fatimah Nabila Zahra Hadi Suhardjono Hasni Wardahni Herry Nirwanto Herry Nirwanto Herry Nirwanto Indah Pemuda Indarwati Indarwati Irsyad Irsyad Ketut Srie Marhaeni Julyasih Laras Setyowati Marcellino Althaf Maria Rodhya Alfa Nensi Agustina Noni Rahmadhini Nora Augustien Novi Nafisah Najmi Pawana Nur Indah Penta Suryaminarsih Presti Mardiyani Rizky Ika Noviyanti Tri Mudjoko Tri MUJOKO Triwidodo ARWIYANTO Vika Yanti Wilujeng Widayati Windriyanti, Wiwin Wiwik Harjani Yenny WURYANDARI Zakaria Wildan Fachrezzy

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