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Tuty Arisuryanti, Tuty
Laboratory of Genetics, Faculty of Biology, Gadjah Mada University
Biochemistry, Genetics & Molecular Biology Education

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Analisis Kariotipe Ular Trawang (Coelognathus radiatus, (Boie 1827)) Qurniawan, Tony Febri; Arisuryanti, Tuty; Nur Handayani, Niken Satuti
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i2.3047

Abstract

Analysis of Radiated Ratsnakes (Coelognathus radiatus, (Boie 1827) Karyotype. Radiatedratsnake is a reptile which has an economic potential an export comodyty. However, there arefew reports on genetic studies of radiated ratsnake. The aim of this research was to examinechromosome characters of the radiated ratsnake collected from Sewon, Bantul, Daerah IstimewaYogyakarta. Chromosome preparation method used was splash using blood culture. The resultsrevealed that the diploid chromosome number (2n) of radiated ratsnake was 30, classified asmetacentric (chromosome pairs number 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, dan 11), submetacentric(chromosome pairs number 12 and 13), and telocentric (chromosome pairs number 14 and 15).Therefore the karyotype formula of radiated ratsnake was 2n = 2x = 30 = 22 m + 4 sm + 4 t. Theresult showed that the shortest of short arm chromosomes was 0 ?m, the longest one was0.665 ± 0.0504 ?m, the shortest of long arm chromosomes was 0.268 ± 0.0011?m, the longestone was 0.746 ± 0.0059 ?m, the shortest of total length chromosomes was 0.27 ± 0.001 ?m, andthe longest of total length chromosomes was 1.41 ± 0.045 ?m. The R value was 5.267 ± 0.15408indicating that radiated ratsnake from Sewon, Bantul, Daerah Istimewa Yogyakarta haschromosome variation size.Keywords: Coelognathus radiatus, blood culture, chromosome character, karyotype
A new species of Microhyla (Anura: Microhylidae) from Sumatra, Indonesia Atmaja, Vestidhia Yunisya; Hamidy, Amir; Arisuryanti, Tuty; Matsui, Masafumi; Smith, Eric N.
TREUBIA Vol 45 (2018): Vol. 45, December 2018
Publisher : Research Center for Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/treubia.v45i0.3625

Abstract

A new species of frog in the genus Microhyla is described from Sumatra, Indonesia based on molecular and morphological characters. This new species was previously confused with M. achatina, a Javan endemic. This new species is diagnosable from its congeners by possessing a medium size (SVL in adult males 18.20–21.32 mm, in adult females 20.37–25.51 mm), a stout body, a nostril–eyelid length being about half of the snout length, having a single outer palmar tubercle, a tibiotarsal articulation reaching the center of the eye (when the hindlimbs are stretched and adpressed to the body), having finger and toe tips dilated, having the dorsum with medial longitudinal grooves, and excibiting a very thin and short dark stripe on the temporal region above a wider cream stripe, extending from the postorbital area to insertion of forelimb. Additionally, the new species is characterized by possessing relatively little foot webbing. Uncorrected 16S rRNA sequence divergences between the new taxon and sequences for other congeneric species available ranged from 4.8 to 15.0%.
ANALISIS KARIOTIPE ULAR TRAWANG (COELOGNATHUS RADIATUS, (BOIE 1827)) Qurniawan, Tony Febri; Arisuryanti, Tuty; Nur Handayani, Niken Satuti
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i2.3047

Abstract

Analysis of Radiated Ratsnakes (Coelognathus radiatus, (Boie 1827) Karyotype. Radiatedratsnake is a reptile which has an economic potential an export comodyty. However, there arefew reports on genetic studies of radiated ratsnake. The aim of this research was to examinechromosome characters of the radiated ratsnake collected from Sewon, Bantul, Daerah IstimewaYogyakarta. Chromosome preparation method used was splash using blood culture. The resultsrevealed that the diploid chromosome number (2n) of radiated ratsnake was 30, classified asmetacentric (chromosome pairs number 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, dan 11), submetacentric(chromosome pairs number 12 and 13), and telocentric (chromosome pairs number 14 and 15).Therefore the karyotype formula of radiated ratsnake was 2n = 2x = 30 = 22 m + 4 sm + 4 t. Theresult showed that the shortest of short arm chromosomes was 0 ?m, the longest one was0.665 ± 0.0504 ?m, the shortest of long arm chromosomes was 0.268 ± 0.0011?m, the longestone was 0.746 ± 0.0059 ?m, the shortest of total length chromosomes was 0.27 ± 0.001 ?m, andthe longest of total length chromosomes was 1.41 ± 0.045 ?m. The R value was 5.267 ± 0.15408indicating that radiated ratsnake from Sewon, Bantul, Daerah Istimewa Yogyakarta haschromosome variation size.Keywords: Coelognathus radiatus, blood culture, chromosome character, karyotype
Co-Authors AMIR HAMIDY Atmaja, Vestidhia Yunisya Matsui, Masafumi Niken Satuti Nur Handayani Smith, Eric N. Tony Febri Qurniawan