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All Journal The Indonesian Journal of Dental Research Majalah Kedokteran Gigi Indonesia Majalah Obat Tradisional Dental Journal (Majalah Kedokteran Gigi)

Alma Linggar Jonarta

Department Of Oral Biology, Faculty Of Dentistry, Universitas Gadjah Mada

Author-ID : 551884

Dentistry Health Professions Medicine & Pharmacology Public Health

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Articles

Micronucleus frequency of the buccal epithelial cells on pesticide-exposed female farmers in Dieng village, Central Java Yudasari, Innayati Oktiana; Susilowati, Heni; Jonarta, Alma Linggar
The Indonesian Journal of Dental Research Proceeding Book
Publisher : The Indonesian Journal of Dental Research

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Pesticide is a toxic material used to eradicate physical intruder of crop, including pests, diseases, or weeds. Long-term exposure of pesticide to human body may give genotoxic effects at cellular level, as its substances are chemically bond with the components of DNA triggering the damage of DNA and chromosomes. Micronucleus, additional nucleus on the cells, presents as a result of the termination of the chemical bonds of DNA. The aim of this study was to investigate the frequency of micronuclei of buccal epithelial cells on pesticide-exposed female farmers in Dieng Village, Central Java. This research was conducted on 36 female villagers of Dieng Plateau. The first group consisted of 18 subjects who had regularly and directly exposed by pesticides due to their job as farmers. The control group consisted of 18 subjects were unexposed to pesticides. Buccal epithelium cells of all subjects was collected by swabing the right and left buccal mucosa using cytobrush, fixed and stained using Feulgen-Rossenbeck method. The micronuclei frequency was counted per 1000 epithelial cells. Data was analyzed using Independent Sample t-test. The statistical analysis showed a significant difference between exposed and unexposed groups (p= 0,01). It is concluded that there was an increase in the micronucleus frequency of buccal epithelial cells on pesticide-exposed female farmers in Dieng Village. Moreover, direct long-term exposure of pesticides may harm and damage the human body cells at the gene level.

Systemic IL-1β and TNF-α Productions of E. coli Lipopolysaccharide-Induced Periodontitis Model on Rats Alma Linggar Jonarta; Widya Asmara; Indwiani Astuti; Regina TC Tandelilin
The Indonesian Journal of Dental Research Vol 1, No 1 (2010)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Periodontal disease, a common inflammatory oral disease involved periodontal tissues, has been linked with the evidence of some systemic disorders. Recently, periodontal disease has been suspected as a trigger of systemic disorders. Penetration of bacterial products, such as lipopolysaccharide (LPS) may reach into deeper periodontal tissues. Therefore there may affect systemic blood and cytokines production. Interleukin-1β (IL-1β) and Tumour Nuclear Factor-α (TNF-α) are known as pro-inflammatory cytokines. The production of systemic IL-1β and TNF-α of E. coli lipopolysaccharide-induced periodontitis model on rats was investigated in this research. Fifteen male Wistar rats, aged 6-8 weeks used for this study were divided into 3 groups. For group 1 and 2, silk ligature 3/0 were inserted in interdental area between upper right molar 1 and 2. First and second group received solution containing 10μg/ml and 1mg/ml E. coli lipopolysaccharide, respectively, mixedwith 2% carboxymethylcellulose (CMC) diluted in 100μl of phosphate buffer saline (PBS). The solution was topically applied on gingival tissues around the gingival sulcus, a single topical application of solution onceper 2 days for 14 days. Untreated subjects were used as negative control. On day 15, the blood was collected from vena orbitalis, and rats were sacrificed. The blood serum of each group was divided into 2 groups andcultured for 4 hours with or without 20μl of 100ng/ml of E. coli LPS. ELISA techniques were used to measure the cytokine productions of the supernatant. The data was analysed using Repeated Measure ANOVA. This study showed that there was a significant increase of IL-1β production on low dose of LPS compared to control and high dose of LPS groups (p<0.05). Whereas TNF-α not significantly showed increasing trend. The increasing trend of pro-inflammatory cytokine productions, such as IL-1β and TNF-α, on LPS-induced periodontitis model in this experiment supports the previous studies about the contribution of periodontal disease in the pathogenesis of systemic diseases.

The effect of in vitro royal jelly provision on adhesion of Pseudomonas aeruginosa Sifra Kristina Hartono; Tetiana Haniastuti; Heni Susilowati; Juni Handajani; Alma Linggar Jonarta
Majalah Kedokteran Gigi Indonesia Vol 5, No 1 (2019): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic bacterium, which could aggressively infect immunocompromised patients and thus, cause high mortality rate. In addition, P. aeruginosa in oropharynx could be aspirated and cause ventilator associated pneumonia. Royal jelly is one of bee’s products that has been used for therapeutic needs including antibacteria. Adherence factor of P. aeruginosa were flagelum, pili and lectin. The aim of the study was to determine the effect of royal jelly to P. aeruginosa adhesion. Suspension of P. aeruginosa (ATCC® 27853) was incubated at 37 °C for 18 h. Treatment groups were exposed to royal jelly with several concentrations, 2%, 4%, 6%; while distilled water was being used as negative control. Bacterial adhesion test was determined using spectrophotometer λ = 600 nm to measure optical density values of adhered bacterial suspension in tubes. The result of one-way ANOVA showed significant differences (p<0.05) of optical density values among groups indicating that royal jelly affected the bacterial adhesion. LSD results showed significant difference of optical density values between 2%, 4%, and 6% royal jelly compared to distilled water. Six percent of royal jelly had the least optical density value compared to the other groups. In conclusion, royal jelly has the ability to decrease adhesion of P. aeruginosa. Six percent of royal jelly has better ability to decrease adhesion of P. aeruginosa than other concentrations.

Rapid enamel deposition on Sprague Dawley after nano calcium supplementation during pregnancy Heriati Sitosari; Alma Linggar Jonarta; Yustina Andwi Ari Sumiwi; Tetiana Haniastuti
Majalah Kedokteran Gigi Indonesia Vol 4, No 3 (2018): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Calcium is one of the most important minerals needed during hard tissue development. The preparation of this material into nano-sized particle is carried out to enhance the bioavailability and distribution of calcium in the body. Lack of calcium during odontogenesis causes defect in enamel such as hypoplasia and hypomineralization. During amelogenesis, after secretion of organic matrices, enamel mineralization will start in the presence of calcium. The objective of this study was to determine the effect of nano calcium supplementation during pregnancy on enamel development. In this study, 3-month-old female Sprague Dawley were mated and divided into three groups: nano calcium group (A), micro calcium group (B), and negative control group (C). The treatment was started on day 1 of pregnancy to day 1 after birth by intragastric administration method. The mandibles of 6 pups from each group were collected and stained with hematoxylin and eosin. Examination was conducted using microscope. Enamel deposition was measured using Optilab Image Raster® and the data collected was analyzed using t-test. Histological section of mandibular right first molar on Sprague Dawley newborn pups showed that enamel was observed on day 1 after birth but only on the group treated with nano calcium and micro calcium. Statistical analysis performed showed that the difference between the two groups was significant (p<0.05). From this study it can be concluded that the administration of nano calcium during pregnancy leads to rapid enamel deposition on Sprague Dawley pups.

Effect of Red Onion (Allium cepa var ascalonicum) Skin Ethanolic Extract on the Motility and the Adhesion Index of Pseudomonas aeruginosa and Macrophage Phagocytosis Index Irma Prasety Ayu Nugraheni; Derana Widyastika; Sofia Maulida; Heni Susilowati; Alma Linggar Jonarta
Majalah Obat Tradisional Vol 24, No 1 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Red onion skin (Allium cepa var ascalonicum) contains various ingredients that may function as antibacterial agents against microorganisms, as well as anti-inflammatory and immunomodulator agents for host cells, such as macrophages. Pseudomonas aeruginosa found in the oral cavity is commensal bacteria that may turn into opportunistic pathogen by utilizing its virulence factors such as motility and adhesion to the host cell. The purpose of this study was to investigate the effect of red-onion-skin ethanolic extract towards P. aeruginosa ATCC 9027 on the motility and adhesion ability, furthermore, to know its effect on the macrophage phagocytosis. The research was conducted into three parts of experiment using red-onion-skin ethanolic extract. Extract-induced bacterial motility test was carried out on semi-solid media, stained using 0.1% crystal violet, then the radial length of the bacterial movement was measured. The bacterial adhesion index to buccal cells was calculated after incubated for two hours and stained with Gram stain. Phagocytic activity of the host cells on P. aeruginosa was done by exposing the extract to the mouse peritoneal macrophages, then the phagocytosed bacteria were counted after Giemsa staining. Statistical test results from the three experiments showed significant differences between the test groups compared to the control groups (p <0.05). It was concluded that the red onion-skin ethanolic extract not only affects P. aeruginosa by reducing swarming motility and preventing bacterial adhesion to buccal epithelial cells, but also induces the host cells by increasing the ability of macrophage phagocytosis to these bacteria.

Micronucleus frequency in exfoliated buccal cells from hairdresser who expose to hair products Koh Hui Yee; Alma Linggar Jonarta; Regina TC. Tandelilin
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 2 (2015): June 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Hairdresser is one of the fastest growing occupations in today’s society. Hairdresser help styling, cutting, colouring, perming, curling, straightening hair and various treatment to customer. Somehow, hairdresser are constantly exposed to chemical substances such as aromatic amines, hydrogen peroxide, thioglycolic acid, formaldehyde in hair products which can cause damage to human’s genome. Micronucleus is one of the effective biomarker for processes associated with the induction of DNA damage. Purpose: The aim of this study was to determine the micronucleus frequencies in buccal mucosa epithelial cells of hairdresser who were exposed to chemical of hair products. Method: This study was conducted on twenty female subjects, who were divided into 2 groups: exposed and non-exposed (control) group. All subjects recruited were working in the same beauty salon. Buccal cells were obtained from each individual by using cytobrush. The cells were stained with modified Feulgen-Ronssenback method and counting of micronucleus per 1000 cell was done under light microscope. The data were analyzed using independent t-test and one-way Anova (p<0.05). Result: The result showed a significant difference in micronucleus frequency between 2 groups. There were a significantly increase of micronucleus frequency in hairdressers and increase of micronucleus frequency with the longer duration of exposure. Conclusion: It concluded that the chemical substances of hair products had affected the micronucleus frequency ofthe epithelial cells in buccal mucosa of hairdressers.

Co-Authors Derana Widyastika Heni Susilowati Heni Susilowati Heriati Sitosari Indwiani Astuti Innayati Oktiana Yudasari, Innayati Oktiana Irma Prasety Ayu Nugraheni Juni Handajani Koh Hui Yee Regina TC Tandelilin Regina TC. Tandelilin Sifra Kristina Hartono Sofia Maulida Widya Asmara Yustina Andwi Ari Sumiwi

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