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All Journal Biosaintifika: Journal of Biology & Biology Education Bioscientist : Jurnal Ilmiah Biologi Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Sewagati
Endry Nugroho Prasetyo
Departemen Biologi, Fakultas Sains Dan Analitika Data, Institut Teknologi Sepuluh Nopember, Jawa Timur, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Engineering Health Professions Immunology & microbiology Social Sciences

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Journal : Bioscientist : Jurnal Ilmiah Biologi

 1 
Antimicrobial Activity Test of Medicinal Plant Extract Using Antimicrobial Disc and Filter Paper Muhammad Taufiq Hidayat; Maharani Pertiwi Koentjoro; Endry Nugroho Prasetyo
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 1 (2023): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i1.7544

Abstract

Plant extracts contain secondary metabolites such as flavonoids and phenolic compounds with antibiotic activity. Antibiotic activity test can be done by using the Kirby-Bauer disc method which the antibiotic activity is indicated by the formation of a clear zone. This research was conducted using extracts of Moringa oleifera leaves and Opuntia cochenillifera cladode extracted using ethyl acetate as a solvent. Antibiotic activity test against Staphylococcus aureus bacteria was done in Mannitol Salt Agar and Luria Bertani Agar media. The Kirby-Bauer discs used are Antimicrobial Disc Oxoid® and Filter Paper. The results of this study show that the use of the Antimicrobial Disc Oxoid® and Filter Paper shows no statistical difference in clear zone results (t-value = 0,45; p-value = 0,655; with α = 95%). While the Clear Zone results on Mannitol Salt Agar gave better results than Luria Bertani Agar and were statistically significant (t-value = 2,46; p-value = 0,02; with α = 95%). These findings indicate that filter paper can be an inexpensive alternative for the antibiotic test with no significantly different result compared to commercial antibiotic disc. However, an antibiotic test against Staphylococcus aureus is better done on Mannitol Salt Agar (MSA) compared to Luria Bertani Agar (LBA).
Co-Authors Adelia Paramita Efendiy Adian Galihditya Sinarto Wardhana Adidoyo Prakoso Afifatul Jannah Ahmad Mujiburrosyid Ahmad Saifullah Hajar Alfi Nur Iftitah Alvin Maulana Amri Maulana Astrina Dilla Bambang Pramujati Candra Faisal Akbar Dandi Taufiq Noerhidayat Dini Ermavitalini Enrique Gianfranco Hamdan Ihsan Haryanto Haryanto Heru Sukadri Hidayah Sri Wilujeng Hikmatun Ni'mah Hikmatun Nikmah Indra Cahya Widagda Kevin Surya Rizky Shodiq Krystallyn Gracella Angeline Maharani Pertiwi Koentjoro Maharani Pertiwi Koentjoro Mahfud Mayongga Heriz Febrada Mohamad Emaldi Wilhan Ariawan Muhamad Irfaid Darojat Muhammad Majid Alifan Muhammad Rizky Sanjaya Muhammad Taufiq Hidayat Mukhlis Solehuddin Niki Yuliansari Nuraini Septi Fijianti Ratri Ninda Handayani Risfanali Raja Siti Isna Rachma Wati Siti Zullaikah Triono Bagus Saputro, Triono Bagus Wicaksono, Sigit Tri