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Aminudin Afandhi
University of Brawijaya
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Diversity and Abundance of Insect on Shallot (Allium Ascalonicum L.) with Integrated Pest Management (IPM) and Conventional Patterns Dorkas Wantiawati Manna; Aminudin Afandhi; Hagus Tarno
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.3

Abstract

Research on diversity and abundance of insect on shallot with Integrated Pest Management (IPM) and conventional patterns has been carried out. This study aims to analyze diversity and abundance of insects, damage level of shallot, and relationship between diversity and abundance of insects with growth and production of shallot in IPM and conventional land. This research was conducted in Junrejo village, Batu City in September to December 2017 while identification of insect was carried out at the Laboratory of Plant Pest and Disease, University of Brawijaya. This study uses Yellow pan trap and Pitfall trap methods. The result showed diversity index (H') of IPM land was 2.50 and of conventional land was 2.67, which both are moderate diversity. The abundance of insect on IPM land is higher (8672 individuals) compared to conventional land (3475 individuals). The level of crop damage is lower in IPM land compared to conventional land, while shallot growth and production is higher in IPM land compared to conventional land.
Fungal Endophytic Beauveria bassiana in Chinese Kale Against Plutella xylostella (L.) Larvae Aminudin Afandhi; Silvi Ikawati; Ismatul Baroro; Mufidah Afiyanti; Rose Novita Sari; Handoko Handoko; Tri Suyono; Yogo Setiawan; Hagus Tarno
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.4

Abstract

This study evaluated three methods of inoculation of Beauveria bassiana endophytes from Chinese kale and then infected into Plutella xylostella L to determine the mortality rate. The endophytic B. bassiana using three different inoculation methods including seeds-soaking, leaf-spraying, and soil-wetting. The Chinese kale seeds from Winsa variety, B.bassiana isolates derived from collection planthopper. The endophytic fungus was identified to be B. bassiana based on the analysis of colony morphology. The results of this study included the degree of colonization of Chinese kale plants and the mortality of P. xylostella. The average B. bassiana colonies in leaves were 13.89%, stems 2.77% and roots 5.55%. The results showed on leaves that the seeds-soaking method obtained higher colonization of B. bassiana rate when compared to leaf-spraying and soil-wetting. The highest percentage of P. xylostella mortality was generated from seeds-soaking (45%), soil-wetting (37.5%), leaf-spraying (35%). The B. bassiana fungus which caused the fastest LT50 with a seeds-soaking (409.48 hours), and then soil-wetting (679.15 hours), leaf-spraying (1090.21 hours). The positive effects of seeds-soaking of endophytic B. bassiana and mortality of Plutella xylostell.
Colony Growth, Sporulation, and Viability of Entomopathogenic Fungus Beauveria bassiana (Balsamo) Vuillemin on Various Agricultural Waste as Growing Media Irvan Kurniawan; Aminudin Afandhi; Hagus Tarno
Research Journal of Life Science Vol 9, No 2 (2022): : IN PRESS
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.02.4

Abstract

Beauveria bassiana is an entomopathogenic fungus that is widely produced for the benefit of biological control agents of various insect pests. The current production that is expected is to use cheap media but produce quality isolates. This study aims to determine colony growth, sporulation and viability of Beauveria bassiana conidia in several agricultural waste media. The experiment was compiled in a complete randomized design and replicated four times. B. bassiana in rice bran media added with cricket flour  had the fastest colony growth while B. bassiana in rice husk media was the lowest, lower than the control treatment. In  wheat bran media added cricket flour has slower colony growth than rice bran with cricket flour media similar to control treatment but has the second highest sporulation after rice bran with cricket flour media. The highest viability belongs to B. bassiana which is grown on rice bran with cricket flour media. The production in each treatment medium has a real effect but the addition of cricket flour has a significant influence in the production of quality isolates.
Co-Authors Dorkas Wantiawati Manna Hagus Tarno Handoko Handoko Irvan Kurniawan Ismatul Baroro Mufidah Afiyanti Rose Novita Sari Silvi Ikawati Tri Suyono Yogo Setiawan