Research Journal of Life Science
Vol 8, No 3 (2021)

Fungal Endophytic Beauveria bassiana in Chinese Kale Against Plutella xylostella (L.) Larvae

Aminudin Afandhi (University of Brawijaya)
Silvi Ikawati (University of Brawijaya)
Ismatul Baroro (University of Brawijaya)
Mufidah Afiyanti (University of Brawijaya)
Rose Novita Sari (University of Brawijaya)
Handoko Handoko (Universitas Islam Malang)
Tri Suyono (University Khairun, Ternate)
Yogo Setiawan (University of Brawijaya)
Hagus Tarno (University of Brawijaya)



Article Info

Publish Date
10 Dec 2021

Abstract

This study evaluated three methods of inoculation of Beauveria bassiana endophytes from Chinese kale and then infected into Plutella xylostella L to determine the mortality rate. The endophytic B. bassiana using three different inoculation methods including seeds-soaking, leaf-spraying, and soil-wetting. The Chinese kale seeds from Winsa variety, B.bassiana isolates derived from collection planthopper. The endophytic fungus was identified to be B. bassiana based on the analysis of colony morphology. The results of this study included the degree of colonization of Chinese kale plants and the mortality of P. xylostella. The average B. bassiana colonies in leaves were 13.89%, stems 2.77% and roots 5.55%. The results showed on leaves that the seeds-soaking method obtained higher colonization of B. bassiana rate when compared to leaf-spraying and soil-wetting. The highest percentage of P. xylostella mortality was generated from seeds-soaking (45%), soil-wetting (37.5%), leaf-spraying (35%). The B. bassiana fungus which caused the fastest LT50 with a seeds-soaking (409.48 hours), and then soil-wetting (679.15 hours), leaf-spraying (1090.21 hours). The positive effects of seeds-soaking of endophytic B. bassiana and mortality of Plutella xylostell.

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Journal Info

Abbrev

RJLS

Publisher

Subject

Education

Description

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