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Toberni S Situmorang
Universitas Efarina
Agriculture, Biological Sciences & Forestry Environmental Science

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Isolasi Dan Karakterisasi Bakteri Dari Tanah Bakaran Sampah Toberni S Situmorang; Rahmiati Rahmiati; Helen Anjelina Simanjuntak
Journal of Natural Sciences Vol 3, No 3 (2022): Journal of Natural Sciences November
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v3i3.306

Abstract

Bacteria are microorganisms that are abundant in the environment. Isolation of bacteria has been carried out from various sources of water, air and soil. Soil is a material that is rich in nutrients needed by various organisms including bacteria, which are capable of producing secondary metabolites that can be utilized in various fields. Different sources of isolates will produce different characteristics of the bacteria. This research aims to determine the type and characterization of bacteria originating from burnt waste soil. The research was conducted using a qualitative descriptive method. The soil sample used is burnt waste soil. The results showed that 6 bacterial isolates from burnt waste soil had different characteristics, namely TBS01, TBS02, TBS03, TBS04, TBS05, TBS06. Microscopic identification results showed that the bacterial isolates TBS01 and TBS02 were gram-negative bacteria and the other isolates included gram-positive bacteria. Differences in morphological and microscopic characteristics indicate different types of bacterial isolates.
Potensi Buah Andaliman (Zanthoxylum acanthopodium DC.) sebagai Antibakteri Terhadap Salmonella typhi Helen Anjelina Simanjuntak; Hermawan Purba; Cander Pasaribu; Junius Gian Ginting; Rahmiati Rahmiati; Toberni S Situmorang
Journal of Natural Sciences Vol 4, No 2 (2023): Journal of Natural Sciences Juli
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v4i2.404

Abstract

Typhoid fever is a major disease of death in the medical field caused by Salmonella typhi infection with symptoms including fever, mucosal lesions, vomiting, diarrhea, fluid loss, septicemia, and peritonitis. The use of natural plants to cure infectious diseases such as andaliman fruit. The aim of this study was to determine the antibacterial activity of Andaliman fruit extract on the growth of Salmonella typhi. Experimental research method. The Kirby-bauer method was used for the antibacterial test, the maceration method was used for extraction. The results of phytochemical compounds found flavonoids, tanins, saponins, tannins, alkaloids steroids, and triterpenoids in simplicia and andaliman fruit extracts. All extracts have the potential to inhibit the growth of Salmonella typhi, with the diameter of the inhibition zone consisting of 10.04 mm, 12.22 mm, 15.46 mm and 16.68 mm in the strong category. These doses are 10%, 30%, 50%, and 70%. The analysis shows the potential of Andaliman fruit extract as an antibiotic in the treatment of typhoid fever.
Co-Authors Cander Pasaribu Helen Anjelina Simanjuntak Hermawan Purba Junius Gian Ginting Rahmiati Rahmiati