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All Journal As-syifaa Jurnal Farmasi
St Maryam
Fakultas Farmasi, Universitas Muslim Indonesia, Makassar
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology

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KARAKTERISASI MAKROSKOPIK DAN MIKROSKOPIK SERTA ISOLASI DNA ISOLAT FUNGI ENDOFIT DAUN EKOR NAGA (Rhaphidophora pinnata L.F Schott) St Maryam; Siska Nuryanti; Kartini Eka Fakhriyanti Rahbuddin
As-Syifaa Jurnal Farmasi Vol 14, No 2 (2022): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jifa.v14i2.900

Abstract

 Endophyte fungi generally produce secondary metabolites which have biological activities such as anticancer, antiviral, or antibacterial compounds. The endophytic fungi isolate of FM 2 code is one of the endophytic fungi samples of dragon tail leaf (Rhaphidophora pinnata (L.f.) Schott) because it has the best activities. The research aimed to isolate DNA samples of the dragon-tail leaves and to identify the endophytic fungi isolates through macroscopic and microscopic observations. The significance of DNA isolation can be found through electrophoresis, which will show DNA band under the UV light. The results showed that the sample the endophytic fungi isolates on FM 2 code was identified conventionally by observing its morphological characters through macroscopic and microscopic identification compared to literature, showing the similarities to the genus Mucor sp. which will show DNA band under the UV light. The results showed that the sample of endophytic fungi was isolated by a transiluminator UV lamp indicating that the sample was successfully isolated
AKTIVITAS SENYAWA FLAVANOID EKSTRAK ETANOL BUNGA KERSEN (Muntingia calabura L) SEBAGAI TABIR SURYA Masdiana Tahir; Rahmawati Rahmawati; St Maryam; Putri Nurfauziah; Naura Nazhifah
As-Syifaa Jurnal Farmasi Vol 14, No 2 (2022): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jifa.v14i2.839

Abstract

Chronic exposure to ultraviolet rays from the sun might result in oxidative stress, changes in the structure and composition of the skin. Sunscreen preparations are recommended to be used to prevent or minimize the harmful effects of UV rays on the skin. Sunscreen can be obtained from natural ingredients, one of which is the flower of cherry (Muntingia calabura L.). This study aims to identify the content of flavonoids and test the sunscreen activity of the ethanol extract of cherry flower (Muntingia calabura L.) in vitro using the UV-Vis spectrophotometric method based on the SPF value. Flavonoids are claimed to be capable to blocking ultraviolet (UV) light-induced radicals that have a protective effect against UV radiation, where the SPF value is a universal measure that describes the potency of a UV defense material. The findings of this study showed that the ethanol extract of cherry flowers positively contained flavonoid compounds including the flavonol, flavanone, and anthocyanidin groups. The results of the sunscreen activity test showed that the ethanol extract of cherry flowers had sunscreen activity at a concentration of 0.01% with an SPF value of 10.9 (moderate SPF), 0.05% with an SPF value of 41.6 (high SPF), 0,15% with an SPF value of 45.5 (high SPF). and 0.25% with an SPF value of 48.7 (high SPF). The higher the extract concentration, the higher the SPF value indicating the greater the activity as sunscreen.
Co-Authors Kartini Eka Fakhriyanti Rahbuddin Masdiana Tahir Naura Nazhifah Putri Nurfauziah Rahmawati Rahmawati Siska Nuryanti