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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS)
Muhaimin Rifa'i
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience

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Gynura procumbens Ethanolic Extract Promotes Lymphocyte Activation and Regulatory T Cell Generation In Vitro Dinia Rizqi Dwijayanti; Muhaimin Rifa'i
Journal of Tropical Life Science Vol. 5 No. 1 (2015)
Publisher : Journal of Tropical Life Science

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Abstract

Immune system is a system of biological structures and processes within organism directed to protect against invaded pathogen. Cellular and humoral immune system mediated by immunocompetent cells such as CD4+ T cells, CD8+ T cells, CD4+CD25+ T cells, and B220 cells play important role for maintaining immunological surveillance. The purpose of this study was to determine the effect of ethanolic extract of  G. procumbens leaves (EEGL) on the profile of CD4+ T cells, CD4+CD25+ T cells, and B220+ cells. Splenic cells were isolated from BALB/c mice and cultured in RPMI1640 medium in the presence of EEGL. After 4 days of incubation, cells were harvested, stained with antibodies and analyzed by flow cytometer. The data were analyzed by one-way ANOVA with α= 0.05 and Tukey test using SPSS 16.0 for windows. The results showed that the extract of  G. procumbens could increase proliferation of CD4+CD62L T cell, CD4+CD25+T cells, and B220+ cells compared to the control. Here, we showed the biological effect of G. procumbensas medicinal herb with immunomodulatory activity andthe dose of 0.1 µg/ml and 1.0 µg/ml could promote T cell activation compared to the highest dose of 10 µg/ml. In terestingly, the dose of 10 µg/ml rather promote than inhibit B cell proliferation.
Modulation of Granulocyte Cells Development by VipAlbumin® Administration in BALB/C Mice with Diabetes Mellitus Andi Rizki Adi Pradana; Mansur Ibrahim; Muhammad Sasmito Djati; Muhaimin Rifa'i
Journal of Tropical Life Science Vol. 5 No. 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.05

Abstract

Diabetes mellitus is a metabolic disease that is caused either by the decrease of insulin secretion frompancreatic β cells or the insensitivity of target cells against insulin. High glucose levels (hyperglycemia condition)can trigger the formation of free radicals, the main cause of diabetes micro and macrovascular complications. Theformation of free radicals and AGE (advanced glycation end-products) is assumed to became the key factor in thedecline of granulocyte cell production as well as the disruption of these cells functional activity. The purpose ofthis research was to determine the role of VipAlbumin® in inhibiting the adverse effects of increased blood glucoselevels, which highly influence the production of granulocyte. This study was divided into in vitro and in vivostage. BALB/C mice were used as experimental animals at in vivo stage and induced to undergo diabetes through100 mg/kg BW streptozotocin (STZ) injection at the age of 5 days. VipAlbumin® administered orally for 14 days,which began when mice reached the age of 14 weeks. The administration of VipAlbumin® divided into 3 dosesi.e. 0,01664 mg/gr BW (1st dose), 0,416 mg/gr BW (2nd dose), and 10,4 mg/gr BW (3rd dose). The further step wasa flowcytometric analysis to see the development of granulocyte cells relative amount, which were isolated fromthe bone marrow. The result of this analysis shows that VipAlbumin® administration, particularly at the 2nd and3rd dose, were able to modulate granulocyte cells development in the bone marrow.
Antioxidant Activity of Unripe Sapodilla Fruit Extract (Manilkara zapota L.) through Nrf2 and SOD Expression in Type 1 Diabetic Mice: Antioxidant activity of Manilkara zapota extract in T1DM Muhaimin Rifa'i; Fikriya Novita Sari; Rizky Senna Samoedra; Setyaki Kevin Pratama; Sri Rahayu; Aris Soewondo; Muhammad Halim Natsir
Journal of Tropical Life Science Vol. 13 No. 1 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.01.12

Abstract

This research aims to analyze the effect of unripe sapodilla fruit extract on endogenous antioxidant expression in T1DM BALB/c mice and its free radical scavenging activity. Manilkara zapota extract (MzE) is an aqueous extract of unripe sapodilla fruit and was obtained by maceration and freeze-drying process. This study used 25 male BALB/c mice with 7-weeks-old of age. They were divided randomly into five groups (n=5) before treatment. A single high dose (145 mg/kg BW) of streptozotocin (STZ) was intraperitoneally injected to induce type 1 diabetes mellitus (T1DM). MzE was given orally once each day for 14 days. Liver cells were isolated and immunoassay with anti-superoxide dismutase (SOD) and anti-nuclear factor erythroid 2-related Factor 2 (Nrf2), and then the results were analyzed by flow cytometry. Diphenylpicrylhydrazyl (DPPH) assay was performed to analyze free radical scavenging. Data were analyzed statistically with one-way ANOVA (p<0.05). The result showed that the glucose levels in diabetic mice after MzE administration were significantly lower than in the DM group. MzE treatment increased the expression of Nrf2 and SOD in diabetic mice. MzE could scavenge DPPH with the IC50 value obtained at 48.35 μg/mL, while ascorbic acid as a control could scavenge DPPH with the IC50 value at 22.24 μg/mL. The increase in the scavenging activity is in line with the increase in extract concentration. In conclusion, this study revealed that MzE can be an endogenous antioxidant enhancer by improving the expression of Nrf-2, SOD and can inhibit free radicals as an exogenous antioxidant in T1DM.
Anti-hyperglycemic and Immunomodulatory Activity of a Polyherbal Composed of Sesbania grandiflora, Salacca zalacca and Acalypha indica Ahmad Zamroni; Elok Zubaidah; Muhaimin Rifa'i; Simon Bambang Widjanarko
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2018.008.03.09

Abstract

Diabetes has become a serious global public health problem due to its high prevalence and mortality. Unfortunately, current anti-diabetic drugs are having some limitations and adverse effects. Therefore, searching for a new anti-diabetic agent is an urgent challenge. In this research, we examined the effectiveness of a traditional anti-diabetic polyherbal composed of Sesbania grandiflora seeds, Salacca zalacca leaves and Acalypha indica roots (2:1:1). The study was aimed to explore the anti-hyperglycemic effect of the polyherbal in STZ-induced diabetic mice and to investigate the immunomodulatory activity involved in the process of controlling hyperglycemia. Our results showed that the polyherbal water extract (150 mg.kgBW-1) could suppress blood glucose elevation and preserve pancreatic islet of diabetic mice. Moreover, the polyherbal treatment could normalize the relative amount of activated CD4+CD62L- and CD8+CD62L- T cells. The polyherbal extract also stimulated the production of CD4+CD25+FoxP3+ regulatory T cells (Tregs) which is known to play an important role in diabetes control. In addition, polyherbal treatment also increased the relative amount of anti-inflammatory cytokines IL-10 and TGF-β. These results revealed that the polyherbal extract has an anti-hyperglycemic and immunomodulatory activity that may provide beneficial function in diabetes healing. Keywords: Antidiabetes, Immunomodulator, Polyherbal, Regulatory T cell.
The Effectiveness of Combination of Momordica charantia Extract and Averrhoa bilimbi on Nf-Kb Activation in Mice (Mus musculus) Balb/C Hyperglycemia Models Intan Sartika Risky Sampurna; Muhaimin Rifa'i; Sri Rahayu
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1262.729 KB) | DOI: 10.21776/ub.jels.2019.010.01.11

Abstract

Hyperglycemia is a medical condition when the blood glucose levels increase exceeds the reasonable limit. Hyperglycemia is a typical sign of diabetes mellitus (DM). Indonesia is the sixth-ranked country in the world after China, India, United States, Brazil, and Mexico. Treatment with herbs is currently being developed. Pare (Momordica charantia) and Belimbing wuluh (Averrhoa bilimbi) are plants that found around us. Some studies state that each of these plants can be anti-diabetic. The hyperglycemia can cause an immune system disorder characterized by pancreatic β cell death involving IL-1β, kappa B (NF)-κB nuclear factor, and Fas. The ability of NF-kB activation will affect the number of cytokines expressed by T cells, namely TNF-α, and IFN-γ. The purpose of this study is to determine the effect of NF-kB activation on blood glucose levels in hyperglycemia mice. The results showed that the positive control treatment showed an increase in the number of NF-kB activations in CD4 and CD8 cells. EPBW (combination of Averrhoa bilimbi extract and Momordica charantia) administration results at a dose of 10 mg.kg-1 BW showed a reduction in the amount of activated NF-kB as a substitute for the reduction. In addition, that dose can reduce blood sugar levels in mice hyperglycemia model.
Effects of Bitter Melon (Momordica charantia L.) and Starfruit (Averrhoa bilimbi L.) on Proinflammatory Cytokines Produced by Hyperglycemic Mice Model Bella Pradina Novinda Wardani; Sri Rahayu; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.03

Abstract

Hyperglycemia is a condition of excessive blood glucose in blood plasma caused by damage to the pancreatic beta-cell structure that causes impaired insulin secretion. This study aimed to investigate the combination of Bitter Melon and Star Fruit ethanol extract (SBME) on pro-inflammatory cytokines production in hyperglycemic albino mice models. This study was treated in 25 female albino mice weighing 25-30 and 9 weeks old. STZ was given in a dose of 145 mg.kg-1 BW intraperitoneally. Hyperglycemic mice were given orally with SBME ethanol extract at doses 10, 40, and 160 mg.kg-1 BW for two weeks. After treatment, pro-inflammatory molecules were analyzed by flow cytometry from the splenic cell. This study showed that SBME treatment can reduce the production of pro-inflammatory cytokines especially IL-1b, but increases IL-6 in hyperglycemic mice. After treatment with SBME at a dose of 40, the IL-1b pro-inflammation molecule decreased significantly (p>0,05). It reached a normal physiological level, but the dose has not been able to reduce the IL-6 pro-inflammation molecule significantly. The conclusion of this study was Bitter Melon and Star Fruit ethanol extract (SBME) with a treatment of 40 mg.kg-1 BW can suppress IL-1b pro-inflammatory cytokines in hyperglycemic mice models, but has not been able to reduce the expression of IL-6 pro-inflammatory cytokines. Suggesting this medicinal herb might be a useful strategy for future therapeutic interventions in degenerative diseases or diseases involving cell activation, but a study of doses is needed.Keywords: Averrhoa bilimbi L., Hyperglycemia, Inflammation, Momordica charantia L.
Co-Authors Ahmad Zamroni Andi Rizki Adi Pradana Aris Soewondo Bella Pradina Novinda Wardani Dinia Rizqi Dwijayanti Elok Zubaidah Fikriya Novita Sari Intan Sartika Risky Sampurna Mansur Ibrahim Moch Sasmito Djati Muhammad Halim Natsir Rizky Senna Samoedra Setyaki Kevin Pratama Simon Bambang Widjanarko Sri Rahayu Sri Rahayu