Article Per Year (5 Year)
p-Index From 2019 - 2024
0.444
P-Index
This Author published in this journals
All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Kelautan Tropis
Subagiyo Subagiyo
Department of Marine Science, Faculty of Fisheries and Marine Sciences, Universitas Diponegoro
Earth & Planetary Sciences

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Genetic and Morphological Variation of the Redbelly Yellow Tail Fusilier, Caesio cuning (Bloch, 1971) from the Nyamuk Waters, Karimunjawa Archipelago Nining Nursalim; Agus Trianto; Ni Kadek Dita Cahyani; Nenik Kholilah; Galank Fad'qul Janarkho; Eko Hardianto; Subagiyo Subagiyo
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 27, No 4 (2022): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.27.4.341-348

Abstract

Yellowtail fusilier, Caesio cuning (Bloch, 1971) is the main catch of fishermen on the Nyamuk Island, Karimunjawa. C. cuning also has a unique character in the form of different eye color, white, red, and white-red. These differences were raise question whether the eye colors will be differentiated the morphology, and the genetic diversity of C. cuning. This research aims to study the biodiversity of C. cuning caught by fishermen on Nyamuk Island, Karimunjawa, based on their morphology, and molecular using mtDNA control region gene marker.  This study also wanted to test, if different eye colors observed on the sample also reflected at the genetic level. A unique thing was found in morphological observations in the form of eye colors in C. cuning (white, red and white-red). Measurements of 19 morphometric characters were conducted on 44 samples directly in the field. Twenty four  (24) samples with three different eye colors were selected for molecular assay of locus control region mtDNA in the laboratory. Morphological analysis showed the total length of 44 samples of C. cuning ranged from 15-29.4 cm. The total length of white eye color ranged from 15-29.4 cm from 20 samples, red-eye color 17-26 cm from 20 samples and white-red eye color 22-24.6 from 4 samples. Molecular test showed that the fish observed was indeed C. cuning with a similarity of 97.07%-99.27% of NCBI data. The genetic distance C. cuning between individuals was 0-3%.  Genetic distance in the same eye color and different have the same value of 2%. Twenty haplotypes were obtained from 24 samples with high haplotypes diversity, values ranging from 0.978-1,000. The nucleotide diversity ranges from 0.019-0.021. There is no molecular difference between C. cuning with different eye colors.
Carrageenan Extraction of Kappaphycus alvarezii Seaweed from Nusa Lembongan Waters Using Different Alkaline Treatments Ichoun Brawici Dhewang; Ervia Yudiati; Subagiyo Subagiyo; Rabia Alghazeer
Jurnal Kelautan Tropis Vol 26, No 2 (2023): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v26i2.17389

Abstract

Kappaphycus alvarezii is a carrageenan-producing red seaweed that is widely cultivated in Nusa Lembongan waters, Bali, Indonesia. Carrageenan is generally extracted using an alkaline base,. iIn this study three different types of alkali were used to extract carrageenan originating from Nusa Lembongan Waters. This study aims to determine the quality of the extraction. The three alkalis used were KOH, NaOH, Ca(OH)2, and distilled water as a control. The 20 g dried seaweed was weighed, washed with the tap running water, and immersed in 0.15% alkaline solution (KOH, NaOH, Ca(OH)2) as well as distilled water for 24 hrs. Followed by rinsing with running water until pH was neutral. The solution was soaked and heated at 100ºC for 2 hours with a ratio of seaweed and water 1 kg in 20 L, and filtered. The extract was mixed with 1.25% KCI. The carrageenan precipitate was put in the oven at 60-80ºC until dry for 48 hours. The yield test results showed that the use of alkaline Ca(OH)2 resulted in a yield of 29.28% better than alkaline KOH (28.18%) and NaOH (27.7%). Based on the analysis of Fourier Transform Infrared Spectroscopy (FTIR), extraction using alkaline Ca(OH)2 showed functional groups characteristic of iota-carrageenan, whereas using alkaline KOH and NaOH showed functional groups characteristic of kappa-carrageenan.
Co-Authors Agus Trianto Eko Hardianto Ervia Yudiati Galank Fad'qul Janarkho Ichoun Brawici Dhewang Nenik Kholilah Ni Kadek Dita Cahyani Nining Nursalim Rabia Alghazeer