R. Priyanto
Department of Animal Production and Technology, Faculty of Animal Science, IPB University

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The Association of Single Nucleotide Polymorphism -69T>G HSPA1A Gene with Bali Cattle Heat Tolerance I. Suhendro; J. Jakaria; R. Priyanto; W. Manalu; R. R. Noor
Tropical Animal Science Journal Vol. 45 No. 4 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.4.429

Abstract

Heat shock protein plays an essential role in thermoregulatory during heat stress responses. This study aims to determine the association of single nucleotide polymorphism (SNP) -69T>G in the promoter region of the heat shock protein 70 member 1A (HSPA1A) gene on heat tolerance in Bali cattle. One hundred and sixteen heads of Bali cattle were collected from different locations such as Pangyangan, Bali Island; Serading, Sumbawa Island; and Sembalun, Lombok Island. The SNP was analyzed by genotyping using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), which used BstUI enzyme restriction. Physiological responses including respiration rate (Rr), rectal temperature (Tr), heart rate (Hr), heat tolerance coefficient (HTC), and blood glucose level (Glu) were measured. Association analysis was conducted using a general linear model by setting genotype, altitude, and sex as factors. The SNP -69T>G variant of HSPA1A gene found in this study were wild type (TT) with 144 bp & 498 bp; GG with 144, 236, & 262 bp; and TG with 144, 236, 262, & 498 bp. Bali cattle with the GG genotype had lower (p<0.001) Rr and HTC compared to the other genotypes. It could be concluded that physiological performances were lower at high altitudes, and the SNP -69T>G HSPA1A was associated with the physiological performances of Bali cattle. SNP -69T>G of HSPA1A could be utilized for candidate marker-assisted selection of Bali cattle to improve the performance of heat tolerance.
Non-Carcass Composition of Thin Tail Sheep Fed Indigofera Zollingeriana with Different Maintenance System A. R. Jatnika; I. Munandar; Husni; S. Nealma; R. Priyanto; L. Abdullah
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 11 No. 1 (2023): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jipthp.11.1.1-6

Abstract

The composition of non-carcass thin-tailed sheep fed Indigefera zollingeriana with different rearing systems, was evaluated using twenty sheep with an average initial body weight of 18.3 ± 2.01 kg. The variables observed in this study included the weight of the head, feet, skin, tail, liver, spleen, lungs, heart, kidneys, rumen, reticulum, omasum, abomasum, intestines, empty viscera and omnetal fat. The design used in this study was a factorial completely randomized design (CRD) with five replications. Feed is treatment factor one (P1 = grass + commercial concentrate and P2 = grass + Indigefera zollingeriana) and maintenance is treatment factor two (SI = Semi Intensive and I = Intensive). The results of the study were analyzed by analysis of covariance. and the correction factor is the initial weight of the sheep. The results of this study showed that giving P2 to sheep resulted in lower tail weight but higher kidney weight (P<0.05). In addition, there was an interaction effect (P< 0.05) between treatments on the weight of theomasum and abomasum, where the sheep with P1I treatment had the highest omasum and abomasum weights, while the lowest omasum and abomasum weights were in the P2I treatment.
Physical Characteristics of Three Types of Muscles with Different Aging Times K. D. Yulianti; R. Priyanto; H. Nuraini
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 11 No. 2 (2023): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jipthp.11.2.54-59

Abstract

The demand for premium beef continues to increase along with changes in the lifestyle of upper middle class, increasing tourists and expatriates from abroad. Local cattle usually cannot produce premium beef, but aging can improve the quality of meat. This study aimed to characterize the physical quality (pH, cooking loss, water holding capacity, and tenderness) of bali beef with different aging times. This study used three types of muscles, which is Longissimus dorsi, Gluteus medius, and Semitendinosus from bali beef aged ± 3 years and body weight of ± 350 kg. Samples were aged at cold temperatures for 1, 21, and 42 days. A completely randomized design with a 3x3x4 factorial was used in this study. Least Square Means test was applied if the data obtained is significantly different. The results showed that the three types of muscle produced relatively the same physical properties, except for cooking loss. The Longissimus dorsi and Semitendinosus muscles were aged for 21 days to show the best results ofmeat tenderness.