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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Naji Arafat Mahat
Department of Chemistry, Faculty of Science, University Teknologi Malaysia, 81310 Skudai, Johor
Agriculture, Biological Sciences & Forestry Environmental Science

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Effect of microbial inoculant on the oviposition and completion of life cycle of Chrysomya megacephala (Fabricius) and Chrysomya rufifacies (Macquart) infesting rabbit carcasses: Effect of microbial inoculant on Chrysomya megacephala (Fabricius) and Chrysomya rufifacies (Macquart) Naji Arafat Mahat; Nur Amalina Omar; Heo Chong Chin; Mohammad Rahim Kamaluddin; Nor Wajihan Muda
Journal of Tropical Life Science Vol. 13 No. 1 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.01.08

Abstract

This research was conducted to assess the influence of microbial inoculant (EM.1®) on the initial oviposition and developmental pattern of two prevalent necrophagous flies (Chrysomya megacephala and Chrysomya rufifacies) in Malaysia. Nine rabbit carcasses were equally divided into control and two treated groups. The control carcasses were sprayed with deionized water (about 100 mL), whereas the two other treated groups were individually sprayed with two concentrations of EM.1® at 1:500 and 1:100, respectively. Results revealed that the single application of EM.1® on both treated carcasses did not statistically impede oviposition by both necrophagous species, as well as their subsequent developmental patterns (P > 0.05) when compared with that of controls. Therefore, estimating the minimum post-mortem interval (mPMI) using empirical baseline data for the control animal model would remain appropriate for estimating mPMI in cases involving carcasses with a single application of EM.1®
Applications of X-Chromosome Short Tandem Repeats for Human Identification: A Review: Applications of X-Chromosome Short Tandem Repeats for Human Identification Naji Arafat Mahat; Aedrianee Reeza Alwi; Faezah Mohd Salleh; Seri Mirianti Ishar; Mohammad Rahim Kamaluddin; Mohd Radzniwan A. Rashid
Journal of Tropical Life Science Vol. 13 No. 1 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.01.19

Abstract

The forensic DNA profiling technique has tremendously contributed to forensic human identification, an important aspect in forensic investigations. In instances whereby comparison samples are unavailable, utilization of short tandem repeats of X chromosome (X-STRs) may prove useful to resolve complex kinship investigations involving missing persons and mass disasters. Despite such evidential values, the use of X-STRs during investigations remains scarce in many Southeast Asian countries including Malaysia, requiring concerted efforts for establishing forensic statistical support for its diverse populations (especially the admixture populations), standardizing core loci and procedure, improving the knowledge among practitioners as well as developing suitable standard operating procedure for incorporating X-STRs analysis in the overall DNA profiling framework. Hence, this review paper aims to highlight the developments, applications and population data of X-STRs, as well as its challenges and future insights for forensic casework.
Nutritional Properties Evaluation of Blowfly Larvae from Fish and Chicken Wastes for Asian Sea Bass Feed Formulation Application: Nutritional Properties Evaluation of Blowfly Larvae Nurazira Anuar; Nur Faizah Moidu Omar; Zainoha Zakaria; Aemi Syazwani Abdul Keyon; Nor Wajihan Muda; Mohammed Suhaimee Abd. Manaf; Naji Arafat Mahat
Journal of Tropical Life Science Vol. 13 No. 3 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.03.02

Abstract

This present research characterized the proximate nutrient, fatty acids and amino acids compositions of the wild third instar blowfly larvae collected from fish and chicken wastes as well as its mixture, in view of aquaculture feeding for Asian sea bass (Lates calcarifer). Analyses of crude protein, crude lipids, crude fiber, ash, carbohydrates, amino as well as fatty acids were performed. Results revealed that the larvae that fed on the mixed substrates (chicken and fish, 50:50 ratio) had significantly the highest nutritional values (51.47 ± 0.32% of crude protein, 29.4 ± 1.47% of crude fat, 4.81 ± 0.83% of crude fiber, 4.85 ± 0.01% of ash and 12.71 ± 1.67% of carbohydrate) for formulating feeds for Asian sea bass when compared with that of chicken and fish wastes alone (P < 0.05). Similarly, significantly higher percentages of fatty acids (DHA and EPA) and amino acids (Arg, Lys, Met, Trp, His, Val, Ile, Leu and Phe) were observed in the larvae from mixed-substrates when compared with that of the remaining two substrates (P < 0.05). The findings supported the utilization of blowfly larvae harvested on mixed substrate as a possible candidate of nutrients for Asian sea bass feed formulations
Co-Authors Aedrianee Reeza Alwi Aemi Syazwani Abdul Keyon Faezah Mohd Salleh Heo Chong Chin Mohammad Rahim Kamaluddin Mohammad Rahim Kamaluddin Mohammed Suhaimee Abd. Manaf Mohd Radzniwan A. Rashid Nor Wajihan Muda Nor Wajihan Muda Nur Amalina Omar Nur Faizah Moidu Omar Nurazira Anuar Seri Mirianti Ishar Zainoha Zakaria