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The Effect Of Several Concentrations Of Growth Regulatory Substance (ZPT) Auxin NAA (Naphthalene Acetic Acid) On The Root Growth Of Vanila (Vanila planifolia Andrew) Cuttings Raesita Dorhayne Timburas; Arthur G. Pinaria; Edy F. Lengkong
Jurnal Agroekoteknologi Terapan Vol. 4 No. 1 (2023): EDISI JANUARI-JUNI 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jat.v4i1.44100

Vanilla cultivation is generally propagated vegetatively, namely by stem cuttings, but the growth potential is still very low, so special treatment is required, such as giving growth regulators (ZPT) which can stimulate growth. ZPT which is often found in the market is Auxin which functions to stimulate growth and stimulate cell division and enlargement. The use of auxin NAA growth regulator causes faster and longer root formation, forming a strong, compact and fibrous root system. This study was an experimental study using a completely randomized design (CRD) consisting of 7 treatments and 5 replications so that the number of plants was 35 plants. The observed variables measured were root growth time, root length, and root dry weight. The results of the research statistically showed that the ZPT NAA treatment had a significant effect on the root appearance and root length variables, but had no significant effect on the root length variable. Keywords: Vanilla, ZPT, Auxin NAA Abstrak Budidaya vanili umumnya diperbanyak secara vegetatif yaitu dengan stek batang, namun potensi tumbuhnya masih sangat rendah sehingga diperlukan perlakuan khusus seperti pemberian zat pengatur tumbuh (ZPT) yang dapat merangsang pertumbuhan. ZPT yang banyak dijumpai di pasaran adalah Auksin yang berfungsi untuk merangsang pertumbuhan dan merangsang pembelahan dan pembesaran sel. Penggunaan zat pengatur tumbuh auksin NAA menyebabkan pembentukan akar lebih cepat dan lama, membentuk sistem perakaran yang kuat, kompak dan berserat. Penelitian ini merupakan penelitian eksperimen dengan menggunakan metode Rancangan Acak Lengkap (RAL) yang terdiri dari 7 perlakuan dan 5 ulangan sehingga jumlah tanaman adalah 35 tanaman. Variabel pengamatan yang diukur adalah waktu tumbuh akar, panjang akar, dan berat kering akar. Hasil penelitian secara statistik menunjukkan bahwa perlakuan ZPT NAA berpengaruh nyata terhadap variabel kenampakan akar dan panjang akar, namun tidak berpengaruh nyata terhadap variabel panjang akar. Kata kunci: Vanili, ZPT, Auxin NAA

Induction Of Direct Somatic Embriogenesis Of Chrysanthemum In Ms And Naa Media Combined With Some Cytokinin Concentrations Hestia Sualang; Edy F. Lengkong; Pemmy Tumewu
Jurnal Agroekoteknologi Terapan Vol. 4 No. 1 (2023): EDISI JANUARI-JUNI 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jat.v4i1.44247

This study aims to determine the effect of using MS media and ZPT NAA combined with several concentrations of cytokinins, namely BAP and Kinetin on somatic embryogenesis as seen from the growth of shoots of chrysanthemum (Chrysanthemum sp.). This research was conducted from June 2022 to October 2022 at the Genetics Laboratory of the Faculty of Agriculture, University of Sam Ratulangi. This study used a completely randomized design (CRD) with 7 treatments namely A (NAA 2.0 ppm); AB1 (NAA 2.0 ppm + BAP 1.0 ppm); AB2 (NAA 2.0 ppm + BAP 2.0 ppm); AB3 (NAA 2.0 ppm + BAP 3.0 ppm); AK1 (NAA 2.0 ppm + Kinetin 1.0 ppm); AK2 (NAA 2.0 ppm + Kinetin 2.0 ppm); AK3 (NAA 2.0 ppm + Kinetin 3.0 ppm). Each treatment was repeated 7 times resulting in 49 experimental units. Observational data were analyzed using the ANOVA test and continued with the 5% LSD test. The results showed that MS medium and growth regulator NAA combined with cytokinins could induce somatic embryogenesis of chrysanthemum plants, where concentrations of NAA 2.0 ppm and BAP 2.0 ppm had the best effect on the number and height of explant shoots. The concentration of NAA growth regulator at 2.0 ppm gave the highest number of roots and when combined with cytokinins it reduced the number of roots. Keywords: Growth Regulatory Substances, Somatic Embryogenesis, Chrysanthemum. Abstrak Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan media MS dan ZPT NAA yang dikombinasikan dengan beberapa konsentrasi sitokinin yaitu BAP dan Kinetin terhadap embriogenesis somatik yang dilihat dari pertumbuhan tunas tanaman krisan (Chrysanthemum sp.). Penelitian ini dilaksanakan pada bulan Juni 2022 sampai Oktober 2022 di Laboratorium Genetika Fakultas Pertanian Universitas Sam Ratulangi. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 7 perlakuan yaitu A (NAA 2,0 ppm); AB1 (NAA 2,0 ppm + BAP 1,0 ppm); AB2 (NAA 2,0 ppm + BAP 2,0 ppm); AB3 (NAA 2,0 ppm + BAP 3,0 ppm); AK1 (NAA 2,0 ppm + Kinetin 1,0 ppm); AK2 (NAA 2,0 ppm + Kinetin 2,0 ppm); AK3 (NAA 2,0 ppm + Kinetin 3,0 ppm). Masing-masing perlakuan yang diulang 7 kali menghasilkan 49 satuan percobaan. Data hasil pengamatan dianalisis dengan menggunakan uji ANOVA dan dilanjutkan dengan uji BNT 5%. Hasil penelitian menunjukkan bahwa media MS dan zat pengatur tumbuh NAA yang dikombinasikan dengan sitokinin dapat menginduksi embriogenesis somatik tanaman krisan, dimana konsentrasi NAA 2,0 ppm dan BAP 2,0 ppm memberikan pengaruh terbaik terhadap jumlah dan tinggi tunas ekplan. Konsentrasi zat pengatur tumbuh NAA 2,0 ppm memberikan jumlah akar terbanyak dan apabila dikombinasikan dengan sitokinin dapat menekan jumlah akar. Kata kunci : Zat Pengatur Tumbuh, Embriogenesis Somatik, Krisan

Effect Of Npk Fertilizer On Growth And Production Of Sorghum (Sorghum bicolor (L.) Moench) Plants. Millenia Feyby Klau; Stella M.T Tulung; Edy F. Lengkong
Jurnal Agroekoteknologi Terapan Vol. 4 No. 1 (2023): EDISI JANUARI-JUNI 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jat.v4i1.47139

Sorghum (Sorghum bicolor (L.) Moench) is one type of cereal plant that has a high nutritional content. The increasing number of people in Indonesia every year is increasing this causes the need for food is also increasing. The current national food production has not been able to meet the needs of the community due to experts in the function of paddy fields, low soil fertility and lack of water, so it is necessary to look for food plants that are able to withstand dry land. Sorghum is one of the solutions for food crops that are able to grow on marginal land, wide adaptation, need little water, suitable for planting on dry land and when the weather is hot. This study aims to determine the effect of NPK fertilizer on the growth and production of sorghum. The study was carried out in Tombatu Village, Southeast Minahasa Regency which took place from August to November 2021. The study used a Randomized Block Design (RAK) consisting of five levels of treatment P1 100 kg/ha, P2 200 kg/ha, P3 300 kg/ha, P4 400 kg /ha, P5 500 kg/ha, the treatment was repeated 5 times. Observation variables included dry weight of plantings, planting seed weight, weight of 1000 seeds ,number of seeds filled with plantings, and number of empty seeds from planting . The results showed that a dose of 500 kg/ha of NPK fertilizer gave the best results for dry weight of the plant , planting seed weight, 1000 seed weight and number of seeds filled with plantings, while the number of empty were not affected by the dose of fertilizer given. Keywords: NPK fertilizer, Sorghum (Sorghum bicolor (L.) Moench) Abstrak Sorgum (Sorghum bicolor (L.) Moench) merupakan salah satu jenis tanaman serealia yang memiliki kandungan nutrisi yang tinggi. Peningkatan jumlah penduduk di Indonesia setiap tahun semakin meningkat hal ini menyebabkan kebutuhan pangan juga semakin meningkat. Produksi pangan nasional yang ada saat ini belum mampu memenuhi kebutuhan masyarakat akibat ahli fungsi lahan sawah, kesuburan tanah rendah dan kekurangan air, sehingga perlu dicari tanaman pangan yang mampu tahan pada lahan kering. Tanaman sorgum merupakan salah satu solusi tanaman pangan yang mampu tumbuh di lahan marginal, adaptasi luas, butuh sedikit air, cocok ditanam pada lahan kering dan saat cuaca panas. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian pupuk NPK pada pertumbuhan dan produksi tanaman sorgum. Penelitian dilaksanakan di Desa Tombatu, Kabupaten Minahasa Tenggara yang berlangsung dari bulan Agustus sampai November 2021. Penelitian menggunakan Rancangan Acak Kelompok (RAK) yang terdiri atas lima taraf perlakuan P1 100 kg/ha, P2 200 kg/ha, P3 300 kg/ha, P4 400 kg/ha, P4 500 kg/ha, perlakuan tersebut diulang sebanyak 5 kali. Variabel pengamatan meliputi berat kering pertanaman, berat biji pertanaman, berat 1000 biji, jumlah biji berisi pertanaman, dan jumlah biji hampa pertanaman. Hasil penelitian menunjukkan bahwa pemberian dosis pupuk NPK 500 kg/ha memberi hasil terbaik untuk berat kering pertanaman, berat biji pertanaman, berat 1000 biji dan jumlah biji berisi pertanaman sedangkan jumlah biji hampa pertanaman tidak dipengaruhi oleh dosis pupuk yang diberikan. Kata Kunci: Pupuk NPK, Tanaman Sorgum (Sorghum bicolor (L.) Moench)

CHARACTERIZATION OF PURPLE CORN PLANTS F2 AND F3 SEEDS (Zea mays L.) FREE PONDS OF MANADO YELLOW CORN WITH PURPLE CORN. Ratna Juita; Yefta Pamandungan; Edy F. Lengkong
Jurnal Agroekoteknologi Terapan Vol. 3 No. 1 (2022): EDISI JANUARI-JUNI 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jat.v3i1.38113

This study aims to determine the characteristics of purple corn F2 and F3 seeds (Zea mays L.) free pollinated Manado Kuning corn with purple corn. The research was carried out in the experimental garden (KP) Pandu, Faculty of Agriculture, Sam Ratulangi University, from July 2019 to October 2019. The research was conducted to describe the growth and yield characters of purple corn obtained from open-pollinated Manado Kuning corn with purple corn using 6 genotypes (cobs) of purple corn, namely: G1 = Genotype 1 purple corn (cob 1), G2 = Genotype 2 purple corn (cob 2), G3 = Genotype 3 purple corn (cob 3), G4 = Genotype 4 purple corn (cob 4), G5 = Genotype 5 purple corn (cob 5), G6 = Genotype 6 purple corn (cob 6). The results of the research on growth components showed that the highest plant height and number of cobs were found in the G5 population (102.71 cm and 1.93) and the highest stem diameter was in the G1 population (2.60 cm). While the yield components in the form of cobs and seeds F3 showed that the highest cob length was found in population G2 (11.57 cm), ear diameter in population G1 (3.17 cm), number of rows of seeds and number of seeds per cob were found in population G5 ( 10.13 and 199.47), the weight of dry cobs with husks and weight of dry cobs without husks were found in the G6 population (51.36 gr and 45.10 gr) and the color of the seeds produced were purple, yellow and white with the percentages respectively 88.11%, 14.82%, 13.28% in population G6, G1 and G3 respectively.Keywords: characterization of corn, purple cornAbstrakPenelitian ini bertujuan mengetahui karakteristik tanaman jagung ungu F2 dan biji F3 (Zea mays L.) hasil bersari bebas jagung Manado Kuning dengan jagung ungu. Penelitian dilaksanakan di kebun percobaan (KP) Pandu Fakultas Pertanian Universitas Sam Ratulangi, pada bulan Juli 2019 sampai dengan Oktober 2019. Penelitian dilaksanakan untuk mendeskripsikan karakter perumbuhan dan hasil jagung ungu yang diperoleh dari persarian bebas (open pollinated) jagung Manado Kuning dengan jagung ungu dengan menggunakan 6 genotipe (tongkol) jagung ungu yaitu: G1 = Genotipe 1 jagung ungu (tongkol 1), G2 = Genotipe 2 jagung ungu (tongkol 2), G3 = Genotipe 3 jagung ungu (tongkol 3), G4 = Genotipe 4 jagung ungu (tongkol 4), G5 = Genotipe 5 jagung ungu (tongkol 5), G6 = Genotipe 6 jagung ungu (tongkol 6). Hasil penelitian pada komponen pertumbuhan menunjukkan bahwa tinggi tanaman dan jumlah tongkol tertinggi terdapat pada populasi G5 (102,71 cm dan 1,93) dan diameter batang tertinggi pada populasi G1 (2,60 cm). Sedangkan pada komponen hasil yang berupa tongkol dan biji F3 menunjukkan bahwa panjang tongkol tertinggi terdapat pada populasi G2 (11,57 cm), diameter tongkol pada populasi G1 (3,17 cm), jumlah baris biji dan jumlah biji pertongkol terdapat pada populasi G5 (10,13 dan 199,47), berat tongkol kering dengan kelobot dan berat tongkol kering tanpa kelobot terdapat pada populasi G6 (51,36 gr dan 45,10 gr) dan warna biji yang di hasilkan yaitu ungu, kuning dan putih dengan persentase berturut-turut 88,11%, 14,82%, 13,28% pada populasi G6, G1 dan G3.Kata kunci: karakterisasi jagung, jagung ungu

GROWTH OF POTATO SEEDS (Solanum tuberesum L.) ON MS MEDIA SUBSTITUTED WITH COCONUT WATER Edy F. Lengkong; hizkia mantiri; Arthur G. Pinaria
Jurnal Agroekoteknologi Terapan Vol. 4 No. 2 (2023): EDISI JULI-DESEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jat.v4i2.50675

The tissue culture technique can be the best method of choice because it is fast in propagation and has a relatively short time. This research was conducted using a completely randomized design, with 4 treatments, namely: P0. MS Media 100%, P1. MS Media 90% + 10% Coconut Water, P2. MS Media 80% + 20 Coconut Water, P3. MS Media 70% + 30% Coconut Water. Each treatment was repeated 10 times to obtain 40 experimental units and in each experimental unit/bottle, there were 2 shoot explants. The results of the study that the substitution of Coconut Water in MS Media had a significant effect on plant height parameters but had no significant effect on parameters of number of leaves, number of roots, number of branches, and dry weight of potato plantlets. The best use of coconut water as a substitute for MS media for the growth of potato plantlets is at a concentration of 30%. because it did not differ from the control (100% MS media), especially the parameters of the number of leaves, number of roots, number of branches, and dry tissue of potato plantlets. Keywords: Potato Seeds, MS Media, Coconut water Abstrak Teknik kultur jaringan dapat menjadi pilihan metode terbaik karena cepat dalam perbanyakannya dan memiliki waktu yang relatif singkat. Penelitian ini dilakukan dengan menggunakan rancangan acak lengkap, dengan 4 perlakuan yaitu: P0. MS Media 100%, P1. MS Media 90% + 10% Air Kelapa, P2. MS Media 80% + 20 Air Kelapa, P3. MS Media 70% + 30% Air Kelapa. Setiap perlakuan diulang sebanyak 10 kali sehingga diperoleh 40 satuan percobaan dan pada setiap satuan percobaan/botol terdapat 2 eksplan pucuk. Hasil penelitian bahwa substitusi Air Kelapa pada Media MS berpengaruh nyata terhadap parameter tinggi tanaman namun tidak berpengaruh nyata terhadap parameter jumlah daun, jumlah akar, jumlah cabang, dan berat kering planlet kentang. Pemanfaatan air kelapa sebagai pengganti media MS untuk pertumbuhan planlet kentang yang terbaik adalah pada konsentrasi 30%. karena tidak berbeda dengan kontrol (media 100% MS) terutama pada parameter jumlah daun, jumlah akar, jumlah cabang, dan jaringan kering planlet kentang. Kata Kunci : Benih Kentang, MS Media, Air Kelapa

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