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All Journal The Journal of Experimental Life Sciences (JELS)

Muhaimin Rifa'i

Department of Biology, Faculty of Mathematics and Natural Sciences, University of Brawijaya, Malang, Indonesia

Author-ID : 5918318

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

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Articles

Bioactivity of Purple Yam Tuber (Dioscorea alata L.) on the Level of CD8+and CD8+CD462L+ T cells and Histology of Liver in BALB/c Mice Model of Digestive Allergy Yuyun Ika Christina; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 4 No. 2 (2014)
Publisher : Postgraduate School, Universitas Brawijaya

Purple yam tuber (Dioscorea alata L.) is a family of Dioscoreaceae containing diosgenin which is known as immunomodulatory agent. This study aimed to understand the quantitative changes of naïve and activated memory of T cells on mice model of digestive allergy after orally treated with ethanol extract of purple yam tuber. In this experiment, architecture of hepar histopathology is also observed. Ethanol extract of purple yam tuber with three doses of 0.167 g/kg bw (U1), 2.008 g/kg bw (U2), and 10.039 g/kg bw (U3) are applied. Data were analyzed using One-way ANOVA (p <0.05) and Tukey test using SPSS 16.0 for Windows. Ethanol extract of purple yam tuber triggers the immunocompetent activity of T cells in mice model of digestive allergy. The result showed that the number of memory type T cells in mice model of digestive allergy decreased in lower dose (0.167 g/kg bw (U1). However, the number of naïve T cells, CD8+CD62L+ in mice with digestive allergy after administration of purple yam tuber ethanol extract increased significantly in lower dose (0.167 g/kg bw (U1) compared with positive control (OVA). Dose variations of extract ethanol of purple yam tuber (0.167 g/kg bw) has a significantly effect to shift the T cell status from memory to naïve. Keywords: Digestive allergy, Dioscorea alata L., histopathology, immunomodulatory, subset T cells

Bioactivity of Ethanolic Extract of Propolis (EEP) in Balb/C Mice's CD4+CD25+ and B220+ Lymphocyte Cells Aisyah Zahroh Aden; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 4 No. 2 (2014)
Publisher : Postgraduate School, Universitas Brawijaya

This experiment was aimed to determine the bioactivity of ethanolic extract of propolis (EEP) against the changes in the quantity of CD4+CD25+ and B220+ lymphocytes and determine the optimal dose of EEP to increase the number of CD4+CD25+ and B220+ cells in Balb/c mice. Balb/c mice were divided into four treatment groups: control treatment, treatments of EEP at a dose of 50 mg.kg-1, 100 mg.kg-1, and 200 mg.kg-1 body weight of mice. All mice were dissected after two weeks post treatment. Profiles of lymphocytes from the spleen expressing CD4+CD25+ and B220+ cells were analyzed by flowcytometry using CellQuest software. Data was analyzed by Kruskall Wallis and Mann Whitney statistical test with P<0.05 using SPSS 16.0 for Windows. The results showed that the treatment of dose of 50 mg.kg-1 of EEP can increase relative number of CD4+CD25+ cells significantly, but those cells decrease significantly when we apply the dose of 100 and 200 mg.kg-1. The relative number of B220+ cells increase in the dose of 50 and 200 mg.kg-1 and decrease in the dose of 100 mg.kg-1 compared to the control. This experiment suggest that EEP has bioactivity to modulate the quantity of CD4+CD25+ and B220+ in dependent manner.Keywords: B220+, CD4+CD25+, lymphocyte, propolis

The Role of Moringa oleifera- Ifalmin® Formulation in Regulation of B220+IgM+ and B220+IgG+ in Diabetic Mice Wahyu Isnia Adharini; Ruri Vivian Nilamsari; Noviana Dwi Lestari; Nashi Widodo; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

It has been known that the immunoglobulin levels were altered in diabetes mellitus (DM) conditions. This study aimed to evaluate the levels of immunoglobulins in DM mice after the administration of Moringa oleifera-Ifalmin® formulation (MI). Streptozotocin, at a dose of 145 mg.kg-1, was injected intraperitoneally to experimental mice to obtain diabetic mice. The groups were divided into normal mice, diabetic mice without treatment, diabetic mice with metformin treatment (307.5 mg.kg-1 BW), and diabetic mice with MI treatment at dose 1 (M:I= 800 mg.kg-1 BW: 800 mg.kg-1 BW), dose 2 (M:I= 615 mg.kg-1 BW: 615 mg.kg-1 BW), and dose 3 (M:I= 800 mg.kg-1 BW: 615 mg.kg-1 BW). Mice were orally treated by MI for 14 days. Subsequently, the levels of immunoglobulin IgM and IgG were evaluated using flow cytometry analysis. IgM and IgG levels were significantly lower in the DM group than the normal group. These results indicated that DM altered immunoglobulin levels. MI treatment for 14 days significantly increased the number of IgM and IgG at the level equivalent to the normal group and significantly different as compared to the DM group. Based on the results, MI can be used as an immunomodulatory agent in humoral immunity through the precise regulation of IgM and IgG.

Adaptive Immune Response Stimulation on Nephrolithiasis Mice Model after Treatment of Tempuyung (Sonchus arvensis L.) Leaf Extract Khoirin Maghfiroh; Muhaimin Rifa'i; Sri Widyarti
The Journal of Experimental Life Science Vol. 4 No. 2 (2014)
Publisher : Postgraduate School, Universitas Brawijaya

Calcium crystal accumulation on kidney can cause kidney stone (nephrolithiasis). The oxalate calcium crystal which is deposite on the kidney can trigger inflammation on the epithelial that is able to induce cells death (necrosis). The necrosis is able to cause inflammation and it will affect the body's immune system. Infection agent that comes to the body will be responded by the innate immunity which will be responded later by adaptive immunity. One of herbal agent that is expected to be used to stimulate adaptive immunity response is Tempuyung (Sonchus arvensis) leaf extract. The aim of this study is to find out the change of relative amount of CD4+ T cells, CD8+ T cells, and B (B220) cells on nephrolithiasis mice model after S.arvensis leaf extract. The mice are divided into six groups; control group, placebo, nephrolithiasis, S.arvensis leaf extract for 7 days, nephrolithiasis then it is continued with S.arvensis leaf extract for 7 days, and simultaneous (nephrolithiasis and S.arvensis leaf extract for 3 months). The amount of relative T lymphocyte cells is measured by using BD FACSCalibur FlowcytometerTM. The data is analyzed by using ANOVA one way (p<0.05) using SPSS 16.0 software for Windows. The result shows that there are changes of relative CD4+ T cells, CD8+ T cells, and B (B220) cells on nephrolithiasis mice model after the giving of S.arvensis leaf extract. The treatment of S.arvensis leaf extract on the nephrolithiasis mice model can stimulate the homeostatic activity by suppressing the B cells. Compound of S.arvensis leaf extract that can inhibit of Th1 cells and the increase of Th2 cells by proliferation cells activity. The treatment of S.arvensis leaf extract for 7 days can suppress CD4+. The S.arvensis leaf extract can stimulate adaptive immune response which is caused by immunomodulatory active component.Keywords: Nephrolithiasis, Sonchus arvensis L., leaf extract, adaptive immune response.

Co-Authors Aisyah Zahroh Aden Khoirin Maghfiroh Nashi Widodo Noviana Dwi Lestari Ruri Vivian Nilamsari Sri Widyarti Wahyu Isnia Adharini Yuyun Ika Christina

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