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All Journal The Journal of Experimental Life Sciences (JELS)
Sri Rahayu
University of Brawijaya
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

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Oocyte In Vitro Maturation with Crude Sperm Extract Protein of Bull's Spermatozoa Bilqis Bilqis; Sri Rahayu; Gatot Ciptadi
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (985.453 KB) | DOI: 10.21776/ub.jels.2016.006.01.04

Abstract

Oocyte In vitro maturation (IVM) is one of the important parts for in vitro fertilization (IVF). The success of oocyte maturation is influenced by the composit ion and the quality of IVM medium. Culture medium which used to IVM not only influences the oocyte process to reach metaphase II and proceed the fertilization, but also influences to developmental of an embryo. Crude sperm extract has high-level protein kinase and contains some sperm proteins. Crude sperm extracts expected as natural maturation medium that can increase the success of In Vitro Maturation (IVM). The characterization of crude sperm extracts profile with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Oocyte maturation is observed in the 26th hour from the first culture. The result of crude sperm extract characterization obtained from the protein with the molecular weight is 176.8, 63.2, 58.4, 55.3, 52.4, 49.7, 44.6, 38.02, 36.03, 34.15 and 26.8 kDa. Oocyte maturation with concentration of crude sperm extract 1.5 µg.mL-1 with 71.6% matures oocyte and oocyte maturation with concentration of crude sperm extract 2.5 µg.mL-1 with 75% matures oocyte. Keywords: Crude Sperm Extract, In Vitro Maturation, Oocyte
Estrous Cycle Response in Mice (Mus musculus) with CSE (Crude Sperm Extract) Injected Intraperitoneally Enni Mutiati; Sri Rahayu; Gatot Ciptadi; Moch. Nasich
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (976.426 KB) | DOI: 10.21776/ub.jels.2016.006.01.03

Abstract

Sperm protein has an important role in fertilization process. It becomes antigenic when it is injected to body and can increase TNF-α secretion. TNF-α in blood vesssel can inhibit estradiol synthesis. Estradiol has a significant role in repduction cycle, especially in estrous cycle. The study aims to understand the influence of Crude Sperm Extract (CSE) to mice (Mus musculus) estrous cycle. 16 fertile mices strain Balb-C aged 3-4 months, weighed 20-30 g was divided into 4 groups. P0 is a control group injected by PBS, group P1, P2, and P3 injected by CSE with 1.5 mg.ml-1, 2 mg.ml-1, and 2.5 mg.ml-1. CSE is injected intraperitoneally during mice's  diestrus phase. CSE is injected in day 0, day 12, day 24 and observed daily. The data are descriptively analyzed. The results show that CSE with molecule weight between 26.8-176.8 kDa influences estrous cycle. Keywords: Crude Sperm Extract, estrous cycle, folliculogenesis, Mus musculus
Role of Active Compounds of Bohadschia argus Inhibit Cancer Cell Survival Jantje Wiliem Souhaly; Sri Rahayu; Widodo Widodo
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1142.888 KB) | DOI: 10.21776/ub.jels.2019.009.01.03

Abstract

Sea cucumber is marine biota with a high economic value and also has potential for anti-cancer. The purpose of this study was to explore the mechanism of active compound of Bohadschia argus on regulating cancer cell survival. The B. argus samples were collected from the sea of Kamal Village, West Seram Maluku, then extracted by water. The constituents of water extract of B. argus were examined by LC-MS. The network among active compound and its protein target were determined by Cytoscape app. The result shows that B. argus has several active compounds, such as chondroitin sulfate, holothurin A, holothurin B, and scabraside that might play a role in cancer cell apoptosis, proliferation, and metastasis. Keywords: Active compound, Bohadschia argus, LC-MS.
Co-Authors Bilqis Bilqis Enni Mutiati Gatot Ciptadi Jantje Wiliem Souhaly Moch. Nasich Widodo Widodo