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All Journal The Journal of Experimental Life Sciences (JELS) Research Journal of Life Science
Mintarto Martosudiro
University of Brawijaya
Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Neuroscience

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Fourier Transform Infrared (FTIR) Spectroscopy Method for Fusarium solani Characterization Ifa Maulidah Hasanah; Mintarto Martosudiro; Luqman Qurata Aini; Kestrilia Rega Prillianti; Matheus Randy Prabowo
Research Journal of Life Science Vol 9, No 1 (2022)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.01.3

Abstract

The detection and identification of microorganisms using spectroscopy techniques promise to be of great value because of their sensitivity, rapidity, low expense, and simplicity.  In this study, we used FTIR spectroscopy for the characterization of Fusarium solani. PCR amplification of DNA extracted from these isolates showed the possibility of amplifying PCR products with sizes 559 bp using the ITS1-ITS4 primers. Based on phylogenetic tree analysis, the isolate of F. solani showed a closely relationship to Fusarium solani isolate MN (MH300495.1) with 99.63% similarity.  The study is focused on the carbohydrate structure which can be analyzed in the range of 900 to 1200 cm-1 of FTIR wavenumber.  The spectra of our samples share similarities with one another, although small differences occur in the absorbance value. The band at 1027 cm-1 is assigned to the C-O stretching of glycogen. Meanwhile, at 1042 cm-1 is interpreted as carbohydrate C-O stretching as well. The band around 1073 cm-1 might arise from both chitin C-C stretching and phosphate stretching of nucleic acids. Other vibrations associated with chitin are also found at 1115 cm-1 and 1151 cm-1 which are assigned to C-O-C symmetric stretching and C-O-C asymmetric stretching, respectively.
Effect of Calcium Polysulfide Applications on Severity of Cocoa Pod Rot (Phytophthora palmivora Butl.) and Fungal Diversity Wahyu Widiyasmoro; Mintarto Martosudiro; Liliek Sulistyowati
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1305.655 KB) | DOI: 10.21776/ub.jels.2017.007.02.02

Abstract

Indonesian government gives a high priority for the development and revitalization of cocoa production. In 2012/2013, production of cocoa in Indonesia reached 410,000 ton from 1,774,500 ha (about 231 kg ha-1), lower than Ghana which is 835,000 ton from 1,600,300 ha (about 521 kg ha-1). One of the constraints of cocoa production in Indonesia is pod rot disease caused by Phytophthora palmivora, which can reduce about 90% of production during wet season. Calcium polysulfide was suggested as one of the potential, cheap and save substance to control P. palmivora. The purpose of this study was to evaluate the calcium polysulfide potential to control the disease and its effect on the phyllosphere-fungal diversity. A positive correlation was found between concentration of calcium polysulfide and inhibition of fungal growth in the media. Otherwise, a negative correlation was found between concentration of calcium polysulfide and fungal biomass. Disease severity of cocoa treated by calcium polysulfide 80 mL L-1 every week was 11.67% significantly lower than control, i.e. 68.33%. Furthermore, it was also found that the index value of fungal diversity on the phylosphere decreased by increasing concentration and interval of  calcium polysulfide application.Keywords: Calcium polysulfide, Cocoa, Phytophthora palmivora.
Co-Authors Ifa Maulidah Hasanah Kestrilia Rega Prilianti Liliek Sulistyowati Luqman Qurata Aini Matheus Randy Prabowo Wahyu Widiyasmoro