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All Journal The Journal of Experimental Life Sciences (JELS) Research Journal of Life Science
Mintarto Martosudiro
University of Brawijaya
Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Neuroscience

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Journal : Research Journal of Life Science

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Fourier Transform Infrared (FTIR) Spectroscopy Method for Fusarium solani Characterization Ifa Maulidah Hasanah; Mintarto Martosudiro; Luqman Qurata Aini; Kestrilia Rega Prillianti; Matheus Randy Prabowo
Research Journal of Life Science Vol 9, No 1 (2022)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.01.3

Abstract

The detection and identification of microorganisms using spectroscopy techniques promise to be of great value because of their sensitivity, rapidity, low expense, and simplicity.  In this study, we used FTIR spectroscopy for the characterization of Fusarium solani. PCR amplification of DNA extracted from these isolates showed the possibility of amplifying PCR products with sizes 559 bp using the ITS1-ITS4 primers. Based on phylogenetic tree analysis, the isolate of F. solani showed a closely relationship to Fusarium solani isolate MN (MH300495.1) with 99.63% similarity.  The study is focused on the carbohydrate structure which can be analyzed in the range of 900 to 1200 cm-1 of FTIR wavenumber.  The spectra of our samples share similarities with one another, although small differences occur in the absorbance value. The band at 1027 cm-1 is assigned to the C-O stretching of glycogen. Meanwhile, at 1042 cm-1 is interpreted as carbohydrate C-O stretching as well. The band around 1073 cm-1 might arise from both chitin C-C stretching and phosphate stretching of nucleic acids. Other vibrations associated with chitin are also found at 1115 cm-1 and 1151 cm-1 which are assigned to C-O-C symmetric stretching and C-O-C asymmetric stretching, respectively.
Co-Authors Ifa Maulidah Hasanah Kestrilia Rega Prilianti Liliek Sulistyowati Luqman Qurata Aini Matheus Randy Prabowo Wahyu Widiyasmoro