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All Journal HAYATI Journal of Biosciences Jurnal Dinamika Pengabdian (JDP) Jurnal Bioteknologi & Biosains Indonesia (JBBI) JURNAL GALUNG TROPIKA Jurnal Agrotech International Journal of Agriculture System
Rinaldi Sjahril, Rinaldi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology

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KERAGAMAN GENETIK 22 AKSESI PADI LOKAL TORAJA UTARA BERBASIS MARKA SIMPLE SEQUENCE REPEATS (SSR) Ladjao, Holy Ekklesia; Sjahril, Rinaldi; Riadi, Muh.
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 2 (2018): December 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3100.711 KB) | DOI: 10.29122/jbbi.v5i2.3031

Abstract

Genetic Diversity of 22 Local Rice Accessions from North Toraja Based on Simple Sequence Repeats (SSR) MarkersABSTRACTOne way to explore the potential of local rice is by the characterization that could obtain genetic diversity of that plants. The aim of this study was to obtain the genetic diversity of 22 local rice accession from North Toraja. Twenty-two of local rice accessions from North Toraja were characterized by 30 SSR markers and using NTSYS pc 2.1 program to analyze genetic diversity. The results showed that twenty-six SSR markers that had been analyzed produced some alelles with a size between 106.75-311 bp, the average number of alleles were 3 and the polymorphism rate was 0.53. On coefficient genetic similarity at 0.38, the population formed three clusters. Cluster I and II were dominated by rice that had no hair on the tip of the grain and cluster III were dominated by rice that had hair on the tip of the grain. There were 105 opportunities to crossing between accessions when the genetic distance was above 0.7.Keywords: genetic diversity, local rice, North Toraja, polymorphism rate, SSR markers ABSTRAKSalah satu cara untuk menggali potensi padi lokal adalah dengan karakterisasi. Dengan adanya kegiatan karakterisasi tersebut maka dapat diketahui bagaimana keragaman genetik dari suatu tanaman. Penelitian ini bertujuan untuk mengetahui keragaman genetik dari 22 aksesi padi lokal Toraja Utara. Duapuluh dua aksesi padi lokal Toraja Utara dikarakterisasi menggunakan 30 marka SSR dan dianalisis keragaman genetiknya menggunakan program NTSYS pc 2.1. Hasil penelitian menunjukkan bahwa duapuluh enam marka SSR yang dianalisis memiliki kisaran ukuran alel antara 106.75-311 bp, dengan jumlah alel rata-rata 3 dan tingkat polimorfisme sebesar 0,53. Koefisien kemiripan genetik 0,38 dan terbentuk 3 klaster. Pada klaster I dan klaster II didominasi oleh padi yang tidak memiliki rambut pada ujung gabahnya, dan pada klaster III didominasi oleh padi yang memiliki rambut pada ujung gabahnya. Selain itu, pada jarak genetik diatas 0,7 terdapat 105 peluang persilangan.Kata Kunci: keragaman genetik, marka SSR, padi lokal, tingkat polimorfisme, Toraja Utara
KERAGAMAN GENETIK 22 AKSESI PADI LOKAL TORAJA UTARA BERBASIS MARKA SIMPLE SEQUENCE REPEATS (SSR) Ladjao, Holy Ekklesia; Sjahril, Rinaldi; Riadi, Muh.
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3100.711 KB) | DOI: 10.29122/jbbi.v5i2.3031

Abstract

Genetic Diversity of 22 Local Rice Accessions from North Toraja Based on Simple Sequence Repeats (SSR) MarkersABSTRACTOne way to explore the potential of local rice is by the characterization that could obtain genetic diversity of that plants. The aim of this study was to obtain the genetic diversity of 22 local rice accession from North Toraja. Twenty-two of local rice accessions from North Toraja were characterized by 30 SSR markers and using NTSYS pc 2.1 program to analyze genetic diversity. The results showed that twenty-six SSR markers that had been analyzed produced some alelles with a size between 106.75-311 bp, the average number of alleles were 3 and the polymorphism rate was 0.53. On coefficient genetic similarity at 0.38, the population formed three clusters. Cluster I and II were dominated by rice that had no hair on the tip of the grain and cluster III were dominated by rice that had hair on the tip of the grain. There were 105 opportunities to crossing between accessions when the genetic distance was above 0.7.Keywords: genetic diversity, local rice, North Toraja, polymorphism rate, SSR markers ABSTRAKSalah satu cara untuk menggali potensi padi lokal adalah dengan karakterisasi. Dengan adanya kegiatan karakterisasi tersebut maka dapat diketahui bagaimana keragaman genetik dari suatu tanaman. Penelitian ini bertujuan untuk mengetahui keragaman genetik dari 22 aksesi padi lokal Toraja Utara. Duapuluh dua aksesi padi lokal Toraja Utara dikarakterisasi menggunakan 30 marka SSR dan dianalisis keragaman genetiknya menggunakan program NTSYS pc 2.1. Hasil penelitian menunjukkan bahwa duapuluh enam marka SSR yang dianalisis memiliki kisaran ukuran alel antara 106.75-311 bp, dengan jumlah alel rata-rata 3 dan tingkat polimorfisme sebesar 0,53. Koefisien kemiripan genetik 0,38 dan terbentuk 3 klaster. Pada klaster I dan klaster II didominasi oleh padi yang tidak memiliki rambut pada ujung gabahnya, dan pada klaster III didominasi oleh padi yang memiliki rambut pada ujung gabahnya. Selain itu, pada jarak genetik diatas 0,7 terdapat 105 peluang persilangan.Kata Kunci: keragaman genetik, marka SSR, padi lokal, tingkat polimorfisme, Toraja Utara
Selection of early maturing and high yielding mutants of Toraja Local Red Rice Grown from M2-M3 Population after Ion Beam Irradiation Sjahril, Rinaldi; Trisnawaty, A. R.; Riadi, Muhammad; Rafiuddin, Rafiuddin; Sato, Tadashi; Toriyama, Kinya; Hayashi, Yoriko; Abe, Tomoko
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.258 KB) | DOI: 10.4308/hjb.27.2.166

Abstract

This study aims to obtain the genotype of local Toraja red rice M2 mutants that have the potential to be developed into early maturing varieties and have high yields. Research carried out with a mass selection method consisting of two irradiation treatments, namely: (1) irradiation with a Carbon ion dose of 150 Gy (PL-C); (2) irradiation with Argon ion dose of 10 Gy (PL-A) and control as a comparison. Selected strains of M2 seed population were planted in experimental strains plus 2 strains as control, each row had 50 plants. Selection is done in two stages. The first stage, the selection is based on the criteria of plant growth components, to select rice plants that have early-middle age characters, dwarf-semi-dwarf, and many tillers. The second stage, selected strains from the scoring method were then grouped based on the degree of similarity with the cluster analysis method. Irradiation with ion beams produces mutants with shorter harvest times than their parents, with the harvest time 16-17 days or 10% shorter compared to their parents. This study produced 10 selected strains, 5 strains of carbon ion and 5 strains of argon ion irradiation treatment, and formed 5 groups with a 90% similarity coefficient.
DETERMINASI KAPASITAS INDUKSI POLIPLOIDI MUTAGEN KIMIAWI KOLKISIN DAN BIO-CATHARANTHINE PADA BAWANG MERAH PALU SECARA IN VITRO Azhar, Arini; Sjahril, Rinaldi
Jurnal Agrotech Vol 13 No 1 (2023)
Publisher : FAKULTAS PERTANIAN UNIVERSITAS ALKHAIRAAT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/agrotech.v13i1.116

Abstract

Bawang merah palu (Allium cepa L. Var. Aggregatum) merupakan salah satu komoditas hortikultura yang mempunyai kandungan gizi dan senyawa yang tergolong zat non gizi serta enzim yang berfungsi untuk terapi, meningkatkan dan mempertahankan kesehatan tubuh serta memiliki aroma khas yang digunakan untuk penyedap masakan dan bahan baku utama industri bawang goreng. Penelitian ini bertujuan untuk mendapatkan konsentrasi yang tepat dari mutagen kimia yaitu bio-catharanthine yang paling efektif untuk mendapatkan bawang merah palu poliplodisasi yang dideteksi melalaui analisis sitologi (flow cytometry). Penelitian dilakukan di Laboratorium Biosains dan Bioteknologi Reproduksi Tanaman, Departemen Budidaya Pertanian Fakultas Pertanian dan Unit Perbenihan Tanaman Teaching Industry, Universitas HasanuddinMakassar. Penelitian akan dilaksanakan pada bulan April - Juli 2023. Pada perlakuan bio-catharanthine waktu muncul akar hanya terbentuk pada kalus yang diberikan perlakuan biocathranthine konsentrasi 0,075% dengan lama perendaman 12. Sedangkan pada perlakuan biocatharanthine waktu muncul tunas pada kalus yang diberikan perlakuan bio-cathranthine konsentrasi 0,050 % lama perendaman 3 jam setelah dipindahkan ke media regenerasi 25 (hst). Selama pengamatan berlangsung kalus hanya membentuk akar dan tunas pada kedua kalus tersebut. Dalam penelitian ini, Bio-cathrantine hanya dapat memberi pengaruh dalam pembentukan akar dan tunas pada kalus dengan konsentrasi 0,075% dengan lama perendaman 12 jam dan pada konsentrasi 0,050 % lama perendaman 3 jam pada tanaman bawang merah palu. Hal ini dapat terjadi karena kualitas kalus yang tidak kompak sehingga diperoleh kualitas kalus yang berbeda-beda.
PEMBUATAN SERUM DENGAN MEMANFAATKAN EKSTRAK KULIT GAMAL (Gliricidia sepium) DAN GEL LIDAH BUAYA (Aloe vera) SEBAGAI ALTERNATIF PENGOBATAN LUKA RINGAN Ahmad, A. Sri Sartika Sufiina; Sjahril, Rinaldi; Yanasari, Herlinda; Suria, Muh. Yunus; Fauzan, Achmad; Febrianti, Nur Qadri
Jurnal Dinamika Pengabdian Vol. 9 No. 2 (2024): JURNAL DINAMIKA PENGABDIAN VOL. 9 NO. 2 MEI 2024
Publisher : Departemen Budidaya Pertanian Fakultas Pertanian UNHAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jdp.v9i2.26957

Abstract

Kulit sangat berperan penting dalam kehidupan manusia. Namun, kulit seringkali menjadi permasalahan utama ketika terjadi kecelakaan, yang mana akibat dari kecelakaan tersebut dapat menimbulkan luka. Luka adalah suatu kondisi rusaknya kontinuitas jaringan, struktur dan fungsi anatomis kulit normal akibat adanya proses patologis. Luka saat ini menjadi masalah penting bagi setiap orang dikarenakan dapat menurunkan tingkat kepercayaan diri, sehingga perawatan dan pengelolaan terhadap luka perlu dilakukan secara baik dan teratur. Obat luka yang yang berbahan dasar alami sangat amat sulit buat didapatkan. Kini telah hadir obat luka yang berasal dari 100% bahan alami yaitu obat luka yang berasal dai kulit gamal dan lidah buaya. Kegiatan ini bertujuan untuk pemanfaatan gamal yang hanya diketahui sebagai pakan ternak, dan pemanfaatan lidah buaya yang masih sangat minim dan menciptakan sebuah inovasi baru serum luka dari bahan alami ekstrak kulit gamal dan juga lidah buaya untuk menyembuhkan luka ringan. Dari kegiatan ini diperoleh sebuah produk serum luka berbahan dasar alami dari ekstrak kulit gamal dan lidah buaya yang dapat dimanfaatkan oleh masyarakat. Kata kunci: Gamal, lidah buaya, luka. ABSTRACT Skin plays a very important role in human life. However, skin is often becoming a major problem when an accident occurs, which can result in injuries. of the accident can cause wounds. Wound is a condition of damage to the continuity of tissue, structure, and anatomical function of normal skin as a result of a pathological process. Wounds are currently an important issue for everyone because they can reduce the level of self-confidence so the care and management of wounds need to be done properly and regularly. Wound medications that are made from natural ingredients are very difficult to obtain. Now there is wound medicine that comes from 100% natural ingredients, namely wound medicine from gamal skin and aloe vera. This activity aims to utilize gamal, which is only known as animal feed, and the utilization of aloe vera, which is still very minimal and creates a new innovation. Utilization is still very minimal and creates a new innovation of wound serum from natural ingredients of Gamal skin extract and aloe vera to heal minor wounds. From this activity, a natural-based wound serum product was obtained from gamal skin extract and aloe vera that can be utilized by the community.
BUDIDAYA TANAMAN SAYUR DAN OBAT ORGANIK DI PEKARANGAN Haring, Feranita; Sjahril, Rinaldi; Syaiful, Syatrianty A.; Sahur, Asmiaty
Jurnal Dinamika Pengabdian Vol. 9 No. 1 (2023): JURNAL DINAMIKA PENGABDIAN VOL. 9 NO. 1 OKTOBER 2023
Publisher : Departemen Budidaya Pertanian Fakultas Pertanian UNHAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jdp.v9i1.28216

Abstract

Pengabdian masyarakat ini diharapkan menjadi pemantik untuk menggerakkan kegiatan perekonomian rumah tangga dan menyediakan sumber pangan dan obat yang sehat untuk keluarga. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk mendorong optimalisasi pemanfaatan lahan pekarangan melalui budidaya tanaman sayur dan obat di Kelurahan Adatongeng, Kecamatan Turikale, Kabupaten Maros. Kegiatan dilaksanakan oleh dosen dan mahasiswa Program Studi Agroteknologi Fakultas Pertanian Universitas Hasanuddin dengan melibatkan kelompok wanita tani (KWT) Segar Harum, Koordinator dan Penyuluh Pertanian Balai Penyuluhan Pertanian Kecamatan Turikale Kabupaten Maros. Kegiatan ini dilaksanakan menggunakan metode pembinaan terkait budidaya tanaman sayur dan obat organik di pekarangan melalui kegiatan penyuluhan dan demonstrasi cara pembuatan pupuk asam amino. Hasil penyuluhan dan pelatihan menunjukkan adanya peningkatan pemahaman para peserta tentang pertanian organik, budidaya tanaman sayur dan obat organik di pekarangan, serta pengetahuan dan keterampilan tentang pembuatan pupuk organik. Kata kunci: Pupuk asam amino, pekarangan. ABSTRACT This community service is expected to be a trigger to drive household economic activities and provide healthy sources of food and medicine for families. This community service activity aims to encourage the optimization of the use of yards through the cultivation of vegetable and medicinal plants in the Adatongeng Village, Turikale District, Maros Regency. The activity was carried out by lecturers and students of the Agrotechnology Study Program, Faculty of Agriculture, Hasanuddin University, involving the Segar Harum Women Farmers Group (KWT), Coordinators and Agricultural Extension Officers at the Agricultural Extension Center, Turikale District, Maros Regency. This activity was carried out using coaching methods related to the cultivation of organic vegetable and medicinal plants in the yard through counseling activities and demonstrations on how to make amino acid fertilizers. The results of counseling and training showed an increase in participants' understanding of organic farming, cultivation of organic vegetable and medicinal plants in the yard, as well as knowledge and skills about making organic fertilizer. Keywords: Amino acid fertilizer, yard.
POTENSI 2-IP DAN TDZ PADA PERBANYAKAN TUNAS CABAI KATOKKON (CAPSICUM ANNUUM VAR. CHINENSE) SECARA IN VITRO Ramlan, R; Sjahril, Rinaldi; Mantja, Katriani
Jurnal Galung Tropika Vol 12 No 1 (2023)
Publisher : Fakultas Pertanian, Peternakan dan Perikanan Universitas Muhammadiyah Parepare

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v12i1.1056

Abstract

Katokkon chili is a specific flora location in Toraja, South Sulawesi highlands that is unique compared to other types of chili peppers. Katokkon chili has a powerful and sharp distinctive aroma with a very high level of spiciness. Tissue culture could be a technique that is promising for breeding Katokkon. This study aims to determine the effect of using natural at a dose of potent 2-iP and synthetic TDZ growth regulators at various concentrations on the initiation of embryonic callus from callus explant. This research was conducted at the Laboratory of Plant Bioscience and Reproduction Biotechnology, Department of Agronomy, Faculty of Agriculture, Hasanuddin University, Makassar, in July-November 2022. This study was designed as an experiment it using a Completely Randomized Design (CRD) pattern with six treatments, each with three plicates. Callus was cultured on MS media with concentrations of 2-iP (1.0 mg L-1, 1.5 mg L-1, and 2.0 mg L-1) and TDZ (0.25 mg L-1, 0.5 mg L-1, and 1.0 mg L-1). The results showed that the administration of 2-iP and TDZ only formed roots and did not form buds. It gave 2-iP 1.0 mg L-1 formed roots on day 79 after plant culture with an average root length of 0.55 cm. The administration of TDZ 0.25 mg L-1 roots appeared on day 84 after pant culture with an average root length of 0.3 cm. A treatment of TDZ (1.0 mg L-1) and 2-iP (2.0 mg L-1) gave the best effect on callus weight Katokkon callus were compact in texture on administering 2-iP and TDZ. Callus changes color from white to green (embryogenic), and some experience browning.
Callus Induction of Porang Plants (Amorphophallus muelleri Blume) with 2,4- D on Various Explant Sources In Vitro Haring, Feranita; Sjahril, Rinaldi; Khatima, Khusnul
International Journal of Agriculture System VOLUME 11 ISSUE 2, DECEMBER 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijas.v11i2.4796

Abstract

This study aims to determine the effect of a concentration of 2,4-D added to the Murhasige and Skoog (MS) basic media on porang callus induction and the effect on various explant sources. The study was conducted in the form of experiments in the Laboratory of Bioscience and Plant Reproduction Biotechnology, Department of Agriculture, Faculty of Agriculture, Hasanuddin University from August to December 2021. The experiments were based on a Completely Randomized Design (CRD) pattern arranged in a factorial way with two treatment factors. The first factor was the concentration of 2,4-D consisting of five levels of treatment: without 2,4-D (0.0 mg L-1), 0.5 mg L-1, 1.0 mg L-1, 1.5 mg L-1, and 2.0 mg L-1. The second factor was the source of explants consisting of three levels: the petiole, leaf midrib, and leaf blade. The results after five months showed that the interaction between the concentration of 2.0 mg L-1 2,4-D with the petiole explants had the best effect on callus induction time (12.25 days) and callus weight (2.97 g). The concentration of 2.0 mg L-1 2,4-D had the best influence on the callus induction percentage (83.33%), and petiole-derived explants had the best influence on the callus induction percentage (91.67%). Administration of 2,4-D at a concentration of 2.0 mg L-1 and the petiole-derived explant gave the best results in this study; therefore, it is expected to be applied to the development of porang plant seeds in vitro.
Co-Authors Abe, Tomoko Achmad Fauzan, Achmad Ahmad, A. Sri Sartika Sufiina Azhar, Arini Dermawan, Rahmansyah Febrianti, Nur Qadri Haring, Feranita Haring, Feranita Hayashi, Yoriko Khatima, Khusnul Ladjao, Holy Ekklesia Ladjao, Holy Ekklesia Muhammad Riadi, Muhammad Rafiuddin, Rafiuddin Ramlan, R Riadi, Muh. Riadi, Muh. Rukka, Rusli M. Sahur, Asmiaty Suria, Muh. Yunus SYATRIANTY A. SYAIFUL Tadashi Sato toriyama, kinya Trisnawaty AR Yanasari, Herlinda