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All Journal Jurnal Peternakan Lokal ANGON: Journal of Animal Science and Technology
Efka Aris Rimbawanto
Fakultas Peternakan Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Social Sciences Veterinary

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Subtitusi Konsentrat dengan Daun Gamal (Gliricidia sepium) Pada Ransum Sapi Potong Secara In Vitro terhadap Kecernaan Bahan Kering dan Organik Merryafinola Ifani; Dwi Wijayanti; Efka Aris Rimbawanto; Bambang Hartoyo
Peternakan Lokal Vol 5 No 1 (2023): Jurnal Peternakan Lokal
Publisher : Program Studi Peternakan Universitas Muslim Maros

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46918/peternakan.v5i1.1721

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh subtitusi konsentrat dengan daun Gamal (Gliricidia sepium) terhadap kecernaan bahan kering dan kecernaan bahan organic secara in vitro dengan pakan basal jerami padi dengan rasio konsentrat dan jerami padi 60:40%. Materi yang digunakan dalam percobaan in vitro adalah cairan rumen berasal dari tiga sapi potong di Rumah Potong Hewan Bantarwuni yang pakan basalnya jerami padi, Kecamatan Kembaran, Kabupaten Banyumas. Daun Gamal berumur satu tahun dan dipanen umur 60 hari berasal dari Kebumen, jerami padi varietas Umbul-umbul dan konsentrat. Perlakuan terdiri dari T0: hijauan 40%+konsentrat 60%, T1: hijauan 40%+konsentrat 40%+ daun Gamal 20%, T2: hijauan 40%+konsentrat 20%+ daun Gamal 40%, T3: hijauan 40%+ daun Gamal 60%. Pengujian dilakukan secara in vitro mengunakan rancangan acak lengkap. Variabel terikat yang diukur adalah kecernaan bahan kering dan kecernaan bahan organik. Hasil penelitian menunjukan bahwa meningkatnya daun Gamal menggantikan konsentrat pada ransum sapi potong dapat meningkatkan (P< 0,05) kecernaan bahan kering dan kecernaan bahan organik. Rata-rata kecernaan bahan kering T0 (72,26%) T1 (79,03%) T2 (85,88%) T3 (86,43%) dan kecernaan bahan organik T0 (80,49%) T1 (85,30%) T2 (87,21%) T3 (94,51%). Hasil uji lanjut Dunnet menunjukan perlakuan T3 merupakan hasil terbaik, maka dapat disimpulkan bahwa daun Gamal dapat menggantikan penggunaan konsentrat sebesar 60%.
ISOLASI DAN EKSTRAKSI DAUN KALIANDRA (Calliandra calothyrsus) SEBAGAI KONSENTRAT PROTEIN DAUN DITINJAU DARI TITIK ISOELEKTRIK, ASAM AMINO DAN ZAT ANTINUTRISI Imam Wahyudi; Efka Aris Rimbawanto
ANGON: Journal of Animal Science and Technology Vol 4 No 2 (2022): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1184.9 KB) | DOI: 10.20884/1.angon.2022.4.2.p204-212

Abstract

Background. Research "Isolation and Extraction of Calliandra (Calliandra calothyrsus) Leaf as Leaf Protein Concentrate Judging from Isoelectric Point, Amino Acids and Antinutrients". This study aims to determine the isoelectric point, total amino acid content and the content of antinutrient tannins in the protein concentrate of calliandra leaves extracted and isolated from the protein. Materials and Methods. The research material used was calliandra (Calliandra calothyrsus) leaves. Determination of the isoelectric point using the modified Yatno method (2009), the total amino acid content using the high-performance liquid chromatography (HPLC) method, crude protein analysis using the Kjedhal according to AOAC (2005), and the content of antinutrient tannins using the butanol-HCl method. Results. The results showed that the isoelectric point of the calliandra leaf protein concentrate was reached in the first extraction with 0.05 N NaOH and pH 8 phosphate buffer in the second extraction resulting in 7.2 mg/ml protein precipitate with a crude protein content of 48.94%. The amino acid composition of calliandra leaf protein concentrate was higher than that of soybean meal except for glutamine. Conclusion. The tannin content produced in this study was 0.01% so that the calliandra leaf protein concentrate was safe to use as a protein source in poultry rations.
Co-Authors Bambang Hartoyo Dwi Wijayanti Imam Wahyudi Merryafinola Ifani