p-Index From 2019 - 2024
0.408
P-Index
This Author published in this journals
All Journal JURNAL ILMIAH PLATAX
Desy M. H. Mantiri
Unknown Affiliation

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

Isolation and amplification of 16S rRNA gen of Associated Microbial isolates in Red Algae Kappaphycus alvarezii from Belang, Southeast Minahasa Regency, North Sulawesi Letha L. Wantania; Stenly Wullur; Elvi L. Ginting; Desy M. H. Mantiri; Suzanne L. Undap; Deiske A. Sumilat; Grevo S. Gerung
Jurnal Ilmiah PLATAX Vol. 7 No. 1 (2019): ISSUE JANUARY-JUNE 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.1.2019.22808

Abstract

This study aims to obtain isolates and amplify the associative bacterial 16SrRNA gene in K. alvarezii algae. The K. alvarezii algae was collected from seaweed cultivation area in Belang, Southeast Minahasa, North Sulawesi. Associative bacteria were sampled from K. alvarezii algae, grown in Nutrient Agar and separated based on their morphological characteristics.  Each isolates were extracted their DNA genome and.the16S rRNA gene of each isolate was amplified using PCR.  Eight associative bacterial from K. alvarezii algae were successfully isolated based on morphological characteristics which were dominated by round shape, smooth edges, convex elevation, and white color of the isolates. The results of genomic DNA extraction from each of these isolates were successfully used as templates to amplify the 16s rRNA gene.Key word: Bacteria, Kappaphycus alvarezii, Taxsonomy, 16S rRNAABSTRAKPenelitian ini bertujuan untuk mendapatkan isolat dan mengamplifikasi gen 16SrRNA bakteri asosiatif pada alga K. alvarezii. Metode penelitian diawali dengan pengambilan sampel alga K. alvarezii di lokasi pembudidayaan rumput laut di desa Belang, Minahasa Tenggara, Sulawesi Utara. Selanjutnya, bakteri asosiatif pada alga K. alvarezii tersebut ditumbuhkan dalam media Nutrient Agar, isolat yang tumbuh kemudian dipisahkan berdasarkan karakteristik morfologinya. dan gen 16S rRNA masing-masing isolat diamplifikasi menggunakn PCR.  Delapan isolat bakteri asosiatif pada alga K. alvarezii berhasil diisolasi dengan karakteristik morfologi berbeda yang didominasi dengan bentuk round, tepian smooth, elevasi convex, dan warna putih. Hasil ekstraksi DNA genom dari masing-masing isolat tersebut berhasil digunakan sebagai template untuk mengamplifikasi gen 16s rRNA.Kata Kunci: Bakteri, Kappaphycus alvarezii, Taksonomi, 16S rRNA
Shell Color Classification And Carotenoid Pigments On Littoraria pallescens (Philippi, 1846) From Mangrove Ecosystem Area On Mokupa Village, Tombariri Sub District and Basaan Village, Ratatotok Sub District) Susan M. Sumampouw; Desy M. H. Mantiri; Farnis B. Boneka; Medy Ompi; James J. H. Paulus; Adnan S. Wantasen
Jurnal Ilmiah PLATAX Vol. 6 No. 2 (2018): ISSUE JULY-DECEMBER 2018
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.6.2.2018.20650

Abstract

The purpose of this study was to classify the color of the shell and to know the carotenoid pigment content in Littoraria pallescens based on color classification and population distribution in the mangrove ecosystem area of Mokupa Village, Tombariri Sub district and Basaan Village, Ratatotok Sub district. Sampling directly on mangrove trees as water begins to recede. Identification of L. pallescens species is done by looking at the shape of the shell, the color of the shell, the color of the operculum and the shape of the genital organ. Shell color classification by inserting into the Color Explorer application. Analysis of carotenoid pigments by extraction process with acetone and petroleum ether, further separation of pigment by thin layer chromatography. The results obtained show that L. pallescence dominates life on mangrove trees. Sex was inversely proportional to the two research sites, 53.8% of the male L. pallescens species and 46.2% female in Mokupa waters while from Basaan waters there were 47.1% males and 52.9% females. Color classification based on the percentage of occurrences of constant color that is black (18.5), black orange (16.3) brown black spots (16,3), gray (10.7), angry (6,3), yellow pale (17.8), brown yellow spots (14,1). The detected pigment based on the color classification of the shell is located on the identical and identifiable Rf for all colors is the β-carotene pigment.Keyword : Littoraria pallescence, Carotenoid pigments Tujuan dari penelitian ini adalah untuk mengklasifikasikan warna cangkang dan mengetahui kandungan pigmen karotenoid pada Littoraria pallescens berdasarkan klasifikasi warna dan sebaran populasinya di wilayah ekosistem mangrove Desa Mokupa kecamatan Tombariri dan Desa Basaan Kecamatan Ratatotok. Pengambilan sampel secara langsung pada pohon mangrove saat air mulai surut. Identifikasi spesies L. pallescens dilakukan dengan melihat bentuk cangkang, warna cangkang, warna operculum dan bentuk organ genital.  Pendataan untuk klasifikasi warna cangkang dengan memasukkan ke dalam aplikasi Color Explorer. Analisis pigmen karotenoid melalui proses ekstraksi dengan aseton dan petroleum eter, selanjutnya pemisahan awal pigmen dengan kromatografi lapis tipis. Hasil yang diperoleh menunjukan bahwa L. pallescence mendominasi hidup pada pohon mangrove. Jenis kelamin berbanding terbalik pada dua lokasi penelitian, spesies L. pallescens jantan 53.8% dan betina 46.2% di perairan Mokupa sedangkan dari perairan Basaan terdapat 47.1% jantan dan 52.9% betina. Klasifikasi warna berdasarkan persentase kemunculan warna yang konstan yaitu warna hitam (18,5), hitam oranye (16,3) coklat bercak hitam (16,3), abu-abu (10,7), Marah (6,3), kuning pucat (17,8), kuning bercak coklat (14,1). Pigmen yang terdeteksi berdasarkan klasifikasi warna pada cangkang adalah berada pada Rf yang sama dan yang dapat diidentifikasi untuk semua warna adalah pigmen ß-karoten.Kata kunci : Littoraria pallescence, Pigmen Karotenoid
The biodiversity of macroalgae in the coastal waters of Kora-Kora, East Lembean Sub-District, Minahasa Regency Rene Ch. Kepel; Desy M. H. Mantiri
Jurnal Ilmiah PLATAX Vol. 7 No. 2 (2019): ISSUE JULY - DECEMBER 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.2.2019.23727

Abstract

This study was carried out in coastal waters of Kora-Kora, East Lembean Sub-District, Minahasa Regency with an objective of knowing the taxa composition of macroalgae through morphological studies. Data collection used exploring survey. Results found 10 species that consisted 1 species of red algae (Rhodophyta), 6 species of brown algae (Phaeophyta), and 3 species of green algae (Chlorophyta).Keyword: Macroalgae, Kora-Kora. ABSTRAKPenelitian ini dilakukan di perairan pesisir Kora-Kora, Kecamatan Lembean Timur, Kabupaten Minahasa dengan tujuan untuk mengetahui komposisi taksa makroalga melalui pendekatan morfologi. Pengambilan data dilakukan dengan menggunakan metode survei jelajah. Hasil penelitian menemukan 10 spesies, yang terdiri dari 1 spesies alga merah (Rhodophyta), 6 spesies alga cokelat (Phaeophyta)) dan 3 spesies alga hijau (Chlorophyta).Kata Kunci: makroalga, Kora-Kora.