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Tety Desrita Handayani
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ISOLASI DAN IDENTIFIKASI BAKTERI KITINOLITIK DARI LIMBAH CAIR PEMBEKUAN UDANG Demas Bayu Handika; Tety Desrita Handayani; Reny Guspratiwi
Jurnal Fish Protech Vol 6, No 1 (2023): Jurnal Fish Protech Vol. 6 No. 1 April 2023
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jfp.v6i1.36916

Abstract

Limbah cair industri pembekuan udang merupakan salah satu sumber mikroorganisme pendegradasi kitin yang potensial. Tulisan ini melaporkan pencarian sumber enzim kitinolitik dari limbah industri perikanan. Tujuan dari riset ini adalah mengisolasi bakteri kitinolitik dari instalasi pengolahan limbah cair pembekuan udang dan mengidentifikasi bakteri terbaik penghasil kitinase. Skrining isolat bakteri dilakukan dengan mengevaluasi indeks kitinolitik (IK) pada medium kitin agar (koloidal kitin 2%). Terdapat 5 bakteri dengan indeks kitinolitik terbesar pada medium agar, yakni AR 17 dengan nilai IK 2,59 diikuti isolat bakteri AR 15; AR 18; AR 16 dan IL 08 secara berturut turut dengan nilai IK 2,55; 2,43; 2,32 dan 2,17. Lima isolat ini kemudian diukur nilai aktivitas kitinolitik pada medium kitin cair yang diperkaya koloidal kitin 1% pada suhu 30ºC. Berdasarkan pengujian didapatkan hasil bahwa Isolat AR 18 memiliki aktivitas enzim kitinolitik terbesar. Hasil identifikasi bakteri yang dilakukan secara biokimia menunjukkan bahwa isolat AR18 memiliki kemiripan 83,33% dengan Corynebacterium striatumKata Kunci: bakteri kitinolitik, isolasi, identifikasi, kitinase, Limbah cair pembekuan udang
SIFAT FISIKOKIMIA TEH TALUA INSTAN METODE SPRAY DRYING DENGAN PERLAKUAN JENIS TELUR YANG BERBEDA devi kumala sari; Tety Desrita Handayani; Nela Eska Putri Putri
Jurnal Sains dan Teknologi Pangan Vol 8, No 3 (2023): Jurnal Sains dan Teknologi Pangan
Publisher : JURUSAN ILMU DAN TEKNOLOGI PANGAN, UNIVERSITAS HALU OLEO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jstp.v8i3.39847

Abstract

ABSTRACT Tea talua is a traditional Minangkabau drink made from tea mixed with egg yolks and beaten with sugar and some other additives. Currently there are no instant talua tea drinks available. Instant drinks are usually in powder form served in airtight packages. Powdered drinks are prepared by quick drying with the addition of coating agents. This study aims to determine the effect of different types of eggs on the physicochemical quality of instant egg tea produced. This research method used a completely randomized design (CRD) which consisted of 3 types of treatment and 3 repetitions. This study used a completely randomized design (CRD) with 3 treatments and 3 replications. The treatment given was the difference between egg yolks as raw material for talua tea, including: R = chicken eggs, B = duck eggs, and K = native chicken eggs, so that 9 treatment units were obtained. Data were analyzed using Analysis of Variance (ANOVA) and Duncan's Multiple Range Test (DMRT) at 95% confidence level. The use of different types of eggs did not differ significantly in the proximate test, namely moisture content (2.17+0.40 - 2.41+0.26%), ash content (1.02 + 0.02 - 1.05 + 0.06%), fat content (6.43 + 3.86 - 7.98 +2.10%), protein content (7.74+1.34 - 8.3+0.53%), carbohydrate content (80.48+2.78 - 82.39 +4.47 %)  and the yield (6.70 +0.55 - 8.61 +2.15 % )of instant talua tea produced Keywords: instan drink, egg, tea, teh talua.
PENGARUH PERENDAMAN PRA PENGERINGAN PADA KADAR KALSIUM OKSALAT TEPUNG PORANG DI KABUPATEN LIMA PULUH KOTA, SUMATERA BARAT Tety Desrita Handayani; Neni Trimedona; Elva Amurita Zebua; Rozi Satria Utama
Jurnal Sains dan Teknologi Pangan Vol 8, No 3 (2023): Jurnal Sains dan Teknologi Pangan
Publisher : JURUSAN ILMU DAN TEKNOLOGI PANGAN, UNIVERSITAS HALU OLEO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jstp.v8i3.38093

Abstract

Porang (Amorphophallus oncophyllus) adalah tanaman lokal yang telah banyak dikembangkan sebagai sumber pangan dan juga mendukung konservasi hutan, akan tetapi porang mengandung senyawa kalsium oksalat yang dapat membahayakan konsumen karena dapat menyebabkan rasa gatal dan iritasi pada mulut dan tenggorokan, dan kerusakan ginjal. Tujuan penelitian ini adalah untuk menurunkan jumlah kadar kalsium oksalat produk tepung porang melalui perendaman pra pengeringan irisan porang. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan dua perlakuan yaitu perendaman dengan larutan garam dan perendaman dengan larutan asam asetat. Masing – masing perlakuan mempunyai dua level yaitu 5% dan 10%. Hasil penelitian menunjukkan kadar kalsium oksalat tepung porang tanpa perlakuan adalah sebesar 3,88%. Perlakuan perendaman larutan garam 5% dan 10% dan larutan asam asetat 5% dan 10% dapat menurunkan kadar oksalat masing – masing sebesar 44,59%, 43,28%, 41,73% dan 45,29%. Hasil penelitian ini menunjukkan bahwa perlakuan perendaman irisan porang dengan larutan garam atau asam asetat sebelum pengeringan irisan porang memberikan pengaruh yang signifikan dalam menurunkan kadar kalsium oksalat.
The Potential of Processing Gambir (Uncaria gambir roxb.) Plants in Mado Laoli Village, Gunungsitoli City Elva Amurita Zebua; Tety Desrita Handayani; Nathasa Wiesdania Sihite
Jurnal Sains dan Teknologi Pangan Vol 8, No 3 (2023): Jurnal Sains dan Teknologi Pangan
Publisher : JURUSAN ILMU DAN TEKNOLOGI PANGAN, UNIVERSITAS HALU OLEO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jstp.v8i3.38637

Abstract

The utilization of the gambier plant in Gunungsitoli City is still limited to produce dried gambier leaves as a raw material for betel nut chewing, so it has the potential to be processed into gambier which has high economic value. The study was aimed to determine the quality of gambier produced from gambier plants that grow in Mado Laoli Village, Gunungsitoli City with different drying methods. This study used a completely randomized design (CRD) consisting of 2 treatments, that were P1 (sun drying) and P2 (oven drying) with 3 replications. The results showed that the gambier plant in Mado Laoli Village had a high potential to be processed into gambier because it has produced gambier which have good quality and met the SNI quality requirements. Sun drying produced gambir second quality (15.48% of water content, 2.37% of ash content, 59.36% of catechin content, 12.17% of tannin content, 5.54% of water insoluble material, 3,29% of alcohol insoluble material). Oven drying produced gambir first quality (11.30% of moisture content, 2.18% of ash content, 68.15% of catechin content, 12.24% of tannin content, 6.32% of water insoluble material, 3.77% of insoluble alcohol). Different drying methods had a significant effect on the yield, moisture content, catechin content, water insoluble material and alcohol insoluble material content.
Characteristics of glucomannan of Porang Flour (Amorphophallus oncophyllus) with Various Chemical Extraction Methods Tety Desrita Handayani; Agustina Agustina; Eni Harmayani; Yudi Pranoto
Jurnal Sains dan Teknologi Pangan Vol 8, No 3 (2023): Jurnal Sains dan Teknologi Pangan
Publisher : JURUSAN ILMU DAN TEKNOLOGI PANGAN, UNIVERSITAS HALU OLEO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jstp.v8i3.38413

Abstract

Porang plants are widely cultivated in Indonesia as a potential source of glucomannan. Glucomannan is obtained by separating impurity components such as starch, cellulose, protein, fat, and the rest of the flour bag from porang flour. The purpose of this study was to determine the effect of the isolation method on porang flour to produce high purity glucomannan. In this study, the extraction of glucomannan was carried out by a modified simple centrifugation method with the addition of aluminum sulfate (EAS), sodium metabisulfite (ENM), both (ENMAS), and no treatment (EK). All of the glucomannan obtained was then analyzed for composition, color, transparency, degree of acetylation and viscosity. The characteristics of porang glucomannan were then compared with konjac glucomannan (PKG). The results showed that ENMAS showed the highest value with a purity value of 93.71%, WHC 55.06 g water/g glucomannan, whiteness degree 79.16, and gel viscosity 73000 cps.
Co-Authors Agustina Agustina Demas Bayu Handika Devi Kumala Sari Elva Amurita Zebua Eni Harmayani Nathasa Wiesdania Sihite Nela Eska Putri, Nela Eska Neni Trimedona Reny Guspratiwi Utama, Rozi Satria Yudi Pranoto