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Effect of Pumpkin Soygurt on Microbiota Balance in Diabetes Mellitus Model Rats Annisa Avelia; Didik Gunawan Tamtomo; Yulia Sari
Proceedings of the International Conference on Nursing and Health Sciences Vol 4 No 1 (2023): January-June 2023
Publisher : Global Health Science Group

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/picnhs.v4i1.1681

Abstract

Microbiota imbalance in the gastrointestinal tract causes low-grade systemic inflammation, which triggers metabolic changes and insulin resistance to become diabetes mellitus. This condition can be controlled through synbiotic supplementation through pumpkin soygurt containing lactic acid bacteria and polysaccharides. This study aimed to analyze the effect of complementary therapy using pumpkin soygurt and metformin on the abundance of lactic acid bacteria and Escherichia coli in a rat model of diabetes mellitus. The research design is experimental with a post-test only. Male Wistar rats were divided into six groups; healthy rats (KS), DM rats (K-), DM+metformin rats (K+), DM+metformin+1 mL pumpkin soygurt rats (P1), DM+metformin+ 1.5 mL pumpkin soygurt rats (P2), DM + metformin + 2 mL of pumpkin soygurt (P3) rats. The test material was given for four weeks. The abundance of LAB and Escherichia coli was analyzed using a sample of the contents of the cecum with the method total plate count. Data were analyzed using the One way ANOVA test. This study showed a dysbiosis condition on K- (BAL 6.85 CFU/mL; Escherichia coli 8.80 CFU/mL) dan K+ (BAL 4,99 CFU/ml; Escherichia coli 7.67 CFU/mL). In the treatment group, eubiotics were maintained, with the best conditions in group P2 with a BAL of 7.46 CFU/mL and Escherichia coli 4,62 CFU/mL. The combination of pumpkin soygurt and metformin can maintain the balance of the gastrointestinal microbiota in DM model rats, especially lactic acid bacteria and Escherichia coli.
The Effect of Bamboo Tali Leaf Tea on Leptin Levels in Rats Metabolic Syndrome Ani Rosa Putri Ayu Mujayanah; Yulia Sari; Shanti Listyawati
Proceedings of the International Conference on Nursing and Health Sciences Vol 4 No 1 (2023): January-June 2023
Publisher : Global Health Science Group

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/picnhs.v4i1.1686

Abstract

Metabolic syndrome is a threat to health problems in the world, namely a group of metabolic disorders that occur simultaneously. Obesity is a risk factor for metabolic syndrome. Fat accumulation causes insulin resistance and leptin resistance resulting in weight gain. Tali bamboo leaf tea contains a natural source of antioxidants that can increase insulin sensitivity so that it can reduce leptin levels. This study aims to determine the effect of bamboo1tali leaf tea on leptin levels in rats with metabolic syndrome. This type of research is experimental laboratoric with a Pre-Post Test with a Control Group design. Wistar male white rats, 8 weeks old, and 150 – 250 grams in weight, were fed HFHF (High Fat High Fructose) for 14 days, induced by Nicotinamide and Streptozotocin. Then, the rats were divided into 5 groups, namely negative control, positive control, and 3 treatments of tali bamboo leaf tea (300, 600, and 900 mg/200 g BW) for 28 days. Measurement of leptin levels using the ELISA method.Bamboo leaf tea had a significant effect (p <0.005) on leptin levels in rats with metabolic syndrome. The largest decrease in leptin levels occurred in the TDB3 group which was 82.8% and the lowest decrease in leptin levels occurred in the TDB1 group which was 34.8%. Bamboo leaf tea doses of 300, 600, and 900 mg/200 g BW reduced leptin levels in rats with metabolic syndrome for 28 days.
POTENSI TEH DAUN BAMBU TALI TERHADAP PERUBAHAN INDEKS MASSA TUBUH DAN KADAR TRIGLISERIDA TIKUS SINDROM METABOLIK Ani Rosa Mujayanah; Yulia Sari; Shanti Listyawati
GIZI INDONESIA Vol 46 No 2 (2023): September 2023
Publisher : PERSATUAN AHLI GIZI INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36457/gizindo.v46i2.889

Abstract

Metabolic syndrome is a metabolic disorder such as obesity, hyperglycemia, hypertension, and dyslipidemia that occur simultaneously. The prevalence of metabolic syndrome globally is increasing and the risk of T2DM, cardiovascular, stroke, and death. Metabolic syndrome is related to dyslipidemia due to excess energy intake which can cause oxidative stress and hypertriglyceridemia. Tali bamboo (Gigantochloa apus Kurz.) leaf tea contains antioxidants such as flavonoids, tannins, and saponins which have the potential to reduce BMI and triglyceride levels. This study aims to determine the potency of tali bamboo leaf tea on BMI and triglyceride levels in rats metabolic syndrome. This type of research is laboratory experimental with a control group pre-post test design. 30 male Wistar rats, aged 8 weeks, with a weight of 150 – 250 g, which were induced by High Fat High Fructose (HFHFr) for 14 days, then continued with STZ-NA. Rats were divided into 5 groups namely K- (aquades), K+ (metformin), and 3 doses steeping tali bamboo leaf (150, 300, and 450 mg/100 g BW) for 28 days. The BMI of rats was measured using the index Lee and triglyceride levels using the GPO-PAP method. Data were analyzed using paired t-test and One Way ANOVA. The results showed that there was a significant difference (p < 0,05) between BMI and triglycerise levels before and after treatment. The effective dose to reduce BMI and triglyceride levels is 300 and 450 mg/100 g BW. Tali bamboo leaf tea has the potential to improve BMI and triglyceride levels so that it can be developed as a therapy for metabolic syndrome.ABSTRAK Sindrom metabolik merupakan gangguan metabolisme tubuh seperti obesitas, hiperglikemia, hipertensi, dan dislipidemia yang terjadi secara bersamaan. Prevalensi sindrom metabolik secara global semakin meningkat dan berisiko terjadinya DMT2, kardiovaskular, stroke dan kematian. Sindrom metabolik berkaitan dengan dislipidemia akibat asupan energi berlebih yang menyebabkan stress oksidatif dan hipertrigliserida. Teh daun bambu tali (Gigantochloa apus Kurz.) mengandung antioksidan seperti flavonoid, tanin, dan saponin yang berpotensi dalam menurunkan IMT dan kadar trigliserida. Penelitian ini bertujuan untuk menganalisis potensi teh daun bambu tali terhadap IMT dan kadar trigliserida tikus sindrom metabolik. Jenis penelitian ini penelitian eksperimental laboratorik dengan rancangan pre-post test kelompok kontrol. Subjek penelitian ini adalah 30 ekor tikus Wistar, jantan, umur 8 minggu dengan BB 150 – 250 g yang diinduksi High Fat High Fructose (HFHFr) selama 14 hari, kemudian dilanjutkan STZ-NA. Tikus dibagi menjadi 5 kelompok yaitu K- (aquades), K+(metformin), dan 3 dosis seduhan teh daun bambu tali (150, 300, dan 450 mg/100 g BB) selama 28 hari. Indeks massa tubuh tikus diukur menggunakan rumus indeks Lee dan kadar trigliserida menggunakan metode GPO-PAP. Data dianalisis menggunakan paired t-test dan One Way ANOVA. Hasil penelitian menunjukkan bahwa terdapat perbedaan yang signifikan (p < 0,05) antara IMT dan kadar trigliserida sebelum dan setelah perlakuan selama 28 hari. Dosis efektif untuk menurunkan IMT dan kadar trigliserida adalah 300 dan 450 mg/100 g BB. Pemberian teh daun bambu tali berpotensi dalam memperbaiki IMT dan kadar trigliserida sehingga dapat dikembangkan sebagai terapi untuk sindrom metabolik. Kata kunci: antioksidan, daun bambu, dislipidemia, indeks Lee, sindrom metabolik