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All Journal Jurnal Florea Jurnal Ilmiah Kedokteran Wijaya Kusuma Jurnal SainHealth Medika Kartika : Jurnal Kedokteran dan Kesehatan Jurnal Pengabdian Masyarakat: Humanity and Medicine Berkala Penelitian Hayati
Rini Purbowati, Rini
Biomedical Department And Biomolecular Research, Faculty Of Medicine, Wijaya Kusuma Surabaya University, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary

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CORRELATION BIOFILM WITH INFECTION: IMPLICATIONS FOR PUBLIC HEALTH AND STRATEGIES TO CONTROL Purbowati, Rini
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 5, No 1 (2016): Edisi Maret 2016
Publisher : Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Biofilms are formed in the surfaces of mucosal of the body cavity can be a major source of infection . Infection by microbial biofilm formers are difficult to treat because of their greater resistance to antimicrobial agents than individual cells . Therefore biofilm -related infections and increased the economic burden of the country. The purpose of this literature review is to examine literature on biofilms and biofilm-associated microbes and their contribution to the disease burden of man with the aim of drawing attention to their public health implication.Biofilm are defined as “collections of microorganisms and their associated extracellular products at an interface and generally attached to a biological and non-biological substratum. Biofilm formation are influenced by factors controlling cell attachment, nature of surface, propertis of medium, and properties of the microbial cell surface. The ability of biofilm formation is genetically regulated by ica ABDC operon. Biofilm life cycle include adhesion of cells, formation of microcolonies, formation of biofilm and detachment anddispersal of biofilm. Structure of biofilm consists microorganisms and extracellular polymeric substances with vertical structures of microorganisms sometimes take the form of towers or mushrooms which are separated by interstitial spaces. Bacterial biofilm showed increased resistance to antibiotics, disinfectants and resistant to phagocytosis and other mechanisms of innate and adaptive immune system. Biofilms associated with food borne illness and affect to food security. Biofilms have been implicated in a wide variety of microbial infections in the body such as as urinary tract infections, catheter infections, middle-ear infections, formation of dental plaque, gingivitis, legionellosis ,infections involving contact lenses, and less common but more lethal processes such as endocarditis, infections in cystic fibrosis, and infections of permanent indwelling devices such as joint prostheses and heart valves. It is important for government agencies with a mandate for safeguarding public health and environment to develop and adopt ap propriate health risk assessment and biofilm-specific guidelines that are protective of both public health and the environment.
KEMAMPUAN PEMBENTUKAN SLIME PADA Staphylococcus epidermidis, Staphylococcus aureus, MRSA DAN Escherichia coli Rini Purbowati
Florea : Jurnal Biologi dan Pembelajarannya Vol 4, No 2 (2017)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (418.621 KB) | DOI: 10.25273/florea.v4i2.1647

Abstract

Biofilm merupakan komunitas bakteri yang terorganisasi, terakumulasi dalam metriks polimer (EPS) atau disebut juga slime yang diproduksi oleh bakteri itu sendiri dan mampu melekat pada permukaan hidup maupun tak hidup. Infeksi yang disertai dengan pembentukan biofilm sulit ditangani secara efektif oleh sistem kekebalan tubuh inang dan tahan terhadap pengobatan dengan antimikroba. S. aureus bersama dengan S. epidermidis merupakan agen penyebab infeksi antara lain infeksi implan medis dan infeksi nosokomial di seluruh dunia. Patogenesis infeksi S. epidermidis terkait dengan pembentukan biofilm. Patogenesis S. aureus disebabkan oleh efek gabungan dari faktor ekstraselular dan toksin, bersama dengan sifat invasif strain seperti perlekatan, pembentukan biofilm, dan ketahanan terhadap fagositosis. Metode Congo Red Agar (CRA) sebagai metode fenotipik yang cukup murah dan mudah dengan kriteria evaluasi didasarkan pada analisis visual warna koloni yang tumbuh pada media agar. Penelitian ini bertujuan untuk mengidentifikasi dan menganalisa kemampuan pembentukan slime pada isolat Staphylococcus epidermidis, Staphylococcus aureus, MRSA dan E. coli. Uji pembentukan slime bakteri dengan Metode CRA dilakukan dengan menanam isolat bakteri S. epidermidis, S. aureus (MSSA 1 dan MSSA 2), MRSA 1 dan E. coli (S4 dan S55) yang  telah diremajakan ke media CRA dalam cawan petri dengan metode streak 4 area. Menginkubasi selama 24 jam pada suhu 37° C pada kondisi aerobik. Interpretasi hasil menurut ketentuan sebagai berikut: strain menghasilkan koloni warna hitam pekat, hitam, dan hitam kemerahan dianggap memproduksi slime, sedangkan koloni merah dan kemerahan diklasifikasikan sebagai tidak memproduksi slime. Kesimpulan penelitian ini adalah isolat bakteri S. epidermidis, S. aureus dan MRSA dengan metode CRA (Congo Red Agar) diketahui mampu membentuk slime sedangkan isolat bakteri E. coli menunjukkan hasil yang bervariasi.
ANALISIS NILAI SKOR-T LUMBAL, KADAR TNF-A, DAN HUBUNGANNYA PADA POSTMENOPAUSE OSTEOPOROSIS POSYANDU-LANSIA Sri Lestari Utami; Nini Primadhani Paras Shinta Dewi; Rini Purbowati
Jurnal SainHealth Vol 5, No 2 (2021): September 2021
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v5i2.1522.24-31

Abstract

Indonesian women have four times higher rates of osteoporosis than men. This is caused by aging and the hormone estrogen, which halts production at menopause. Tumor Necrosis Factor-? (TNF-?) and other inflammatory cytokines play a role in the process of bone loss caused by an imbalance of osteoblast and osteoclast activity. This study aims to determine the prevalence of osteoporosis in postmenopausal women at the Elderly Integrated Service Post (Posyandu Lansia) based on the value of Bone Mineral Density (BMD) with lumbar T-score and TNF-levels and to analyze various possible relationships between the two. The research method has two stages. The first stage is screening activities at the Posyandu Lansia according to the inclusion and exclusion criteria using a questionnaire and measuring the BMD (bone mineral density) value with a sonometer. The second stage involves establishing the diagnosis of osteoporosis in 58 postmenopausal women with Dual Energy X-Ray Absorptiometry (DXA) and taking serum for analysis of TNF-? levels. TNF-? levels were determined using the ELISA.The results showed that the prevalence of osteoporosis in postmenopausal women at the Posyandu Lansia was 60.3% based on the value of bone mass density with a lumbar T-score. The percentage of the respondent group with TNF-? levels below the limit value on the standard curve for normal people in the kit (15.6 pg/mL) was 50%. Relationship analysis showed that there was no association between TNF-? levels and BMD values at the lumbar T-score (p value = 0.063 > 0.05). The same thing was also shown in the relationship between the osteoporosis group (Categories 1 and 2) and TNF-? levels (p-values of 0.864 and 0.788 respectively. Analysis of the relationship in this study needs to be carried out more deeply because of the classic and paradoxical effects of TNF-? on the role of osteoclastogenesis.Keywords: osteoporosis, TNF-?, lumbar T-score, postmenopause women
Biofilm formation and detection of A/D genes in MRSA (Methicillin-Resistant Staphylococcus aureus) and MSSA (Methicillin-Sensitive Staphylococcus aureus) Rini Purbowati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 2 (2019): June 2019
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.727 KB) | DOI: 10.23869/25

Abstract

MRSA (Methicillin-Resistant Staphylococcus aureus) arises due to excessive use of methicillin antibiotics and complications from all medical practices that have been carried out lately. The prevalence of MRSA tends to increase and its ability to form biofilms so that it has the potential to significantly increase mortality and morbidity. Biofilms are considered one of the microbial virulence factors. Biofilm formation in S. aureus is regulated by the expression of PIA which mediates attachment between cells to cells and is a product of the ABDC ica gene. This study aims to determine the ability of biofilm formation as one of the virulence factors in MRSA compared to MSSA (Methicillin-Sensitive Staphylococcus aureus) . its relationship to the presence of A and D ica genes as biofilm coding genes. Biofilm formation assay on MRSA and MSSA using the Microtiter Plate (MtP) method and the A/D gene detection assay performed by PCR method. The results showed that the incubation end value of Optical Density (OD) for MSSA1 (0.75), MSSA2 (0.46), MRSA1 (0.53) isolates according to the formula were interpreted as moderate biofilm producer. The conclusions of this study were all of the test isolates (MSSA1, MSSA2 and MRSA1) were able to form a biofilm. Distribution of ica A and ica D genes in MSSA 1 and MSSA 2 was varied while MRSA1 had the ica A and ica D genes.
Antibacterial and antibiofilm effect of silver and gold nanoparticles in Uropathogenic Escherichia coli Rini Purbowati; Sugiharto; Agusniar Furkani Listyawati; Masfufatun; Lusiani Tjandra; Noer Kumala Indahsari
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 2 (2022): June 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.2.20222

Abstract

Nosocomial infections of the urinary tract are the most common nosocomial infections. Infectious diseases currently pose a life threatening due to the increasing phenomenon of infectious agent resistance. One of the factors known to cause resistance to broad-spectrum antimicrobials agent is the susceptibility to biofilm formation. The use of silver nanoparticles (NPs) is now regarded as one of the most promising strategies to target infections associated with biofilms. This study aims to determine the application of nanotechnology in the form of metal nanoparticles in targeting the formation of biofilms on infectious agents. The antibacterial and antibiofilm activity test was performed on a 96-well microtiter plate on uropathogenic Escherichia coli (UPEC) at concentrations of 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 ppm. The results showed the addition of silver and gold nanoparticles at different concentrations affected and inhibited the growth of UPEC and prevented the formation of biofilms, as indicated by a decrease in the OD value. In conclusion, silver and gold nanoparticles have antibacterial and antibiofilm effects on UPEC.
Pelatihan cara cuci tangan yang benar dan penyuluhan cara hidup sehat untuk mencegah terjadinya COVID-19 di SMA Ta’miriyah Surabaya Noer Kumala Indahasari; Olivia Herliani; Rini Purbowati; Lusiani Tjandra; Masfufatun Masfufatun
Jurnal Pengabdian Masyarakat: Humanity and Medicine Vol 2 No 3 (2021): Jurnal Pengabdian Masyarakat: Humanity and Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/Hummed.V2I3.60

Abstract

Proper Hand Washing Training and Healthy Living Counseling for The Perevention of COVID-19 at Ta’Miriyah High School Surabaya. The COVID-19 pandemic has not yet been resolved worldwide, nor in Indonesia. Adequate treatment has not been found, so prevention is a rational step that must be maximized. Proper hand washing is one of the health community movements that can suppress the spread of COVID-19. The hand washing training activity at Ta'miriyah High School Surabaya aims to increase students' knowledge about how to wash hands properly to reduce the spread of COVID-19 in society in general, and in the school environment in particular. The result of this training was monitored through the pre-test and post-test scores performed by students before and after the training. The post-test scores were found to be increased compared to the pre-test scores in all classes. Data analysis stated that there was a significant effect of the hand washing training with the increase of students' knowledge about COVID-19 and how to wash hands properly (p<0.05). The conclusion obtained from these results is that the hand washing training can increase students' knowledge about COVID-19 and how to wash hands properly. This result is expected to have a real impact in preventing the spread of COVID-19 in the Ta'miriyah High School Surabaya.
Peningkatan pengetahuan “Pentingnya Perilaku Hidup Bersih dan Sehat” pada Posyandu Remaja Mojo Gubeng Surabaya Rini Purbowati; Ayly Soekanto; Febtarini Rahmawati; Lusiani Tjandra; Masfufatun; Emilia Devi Dwi Rianti; Noer Kumala Indahsari
Jurnal Pengabdian Masyarakat: Humanity and Medicine Vol 3 No 1 (2022): Jurnal Pengabdian Masyarakat: Humanity and Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/Hummed.V3I1.72

Abstract

Indonesia is currently facing a double burden of diseases, namely Communicable Diseases (PM) and Non-Communicable Diseases (NCD) which are strongly influenced by changes in the environment, community behavior, demographic transition, technology, economy, and socio-culture. To overcome this problem, the government has intensified the Program Perilaku Hidup Bersih dan Sehat (PHBS), which should involve all elements of society, stakeholders, the private sector, academia, and non-governmental organization (NGOs) and other sectors. The role of young people is needed by the government in the process of sustainable development or the Sustainable Development Goals (SDGs). The purpose of this activity is to increase public knowledge and awareness, especially among members of the Youth Posyandu Mojo Gubeng Surabaya about the importance of clean and healthy living behavior. This service activity was carried out on Saturday 11 September 2021, attended by 26 participants. This community service activity was chosen in the form of an online seminar (webinar) to support the government in handling COVID-19 by implementing Emergency PPKM. Measurement of the increase in participants' knowledge was carried out through pre-test and post-test by answering questions related to the webinar material by filling out Google Forms. The measurement results obtained a pre-test average and post-test average value (82.1±18) with a delta value (change in score) of 18.6. Community Service Activities through a Webinar with the title "The Importance of Healthy Lifestyle Behavior at the Youth Posyandu Mojo Gubeng Surabaya" can increase public knowledge at the Mojo Youth Posyandu Surabaya.
IDENTIFIKASI POLA BAKTERI PADA URINE PENGGUNA KATETER DENGAN INFEKSI SALURAN KEMIH DI RSUD DR. SOETOMO SURABAYA I Gede Herry Ananta Wijaya; Rini Purbowati
Medika Kartika : Jurnal Kedokteran dan Kesehatan Vol 5 No 3 (2022): Medika Kartika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran Universitas Jenderal Achmad Yani

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Abstract

Infeksi Saluran Kemih (ISK) merupakan masalah klinis yang sering terjadi baik di masyarakat maupun di kementerian kesehatan terkait. ISK adalah tumbuh dan berkembangnya mikroorganisme abnormal di saluran kemih. Pemeriksaan kultur urine penting dalam menegakkan diagnosa ISK. Penderita yang dikatakan positif ISK bila jumlah bakteri dalam urine > 105 CFU/ml. Tujuan penelitian ini adalah untuk mengetahui jenis mikroba patogen penyebab ISK yang menggunakan kateter di RSUD DR. Soetomo Surabaya Bulan Agustus sampai September 2016. Penelitian ini menggunakan 35 sampel urine ISK yang menggunakan kateter tersebut dilakukan kultur dan pengamatan secara makroskopik, mikroskopik, dan uji biokimia untuk menentukan spesies bakterinya menggunakan KB016 Hi24 Enterobacteriaceae Identification Kit. Hasil penelitian menunjukan bahwa sebanyak 21 penderita ISK adalah perempuan sedangkan 14 penderita ISK adalah laki-laki . Ditinjau dari kelompok usia, kelompok usia 51-70 tahun lebih berisiko mengalami ISK yaitu sebanyak 16 kasus. Pola bakteri pada penderita ISK pengguna kateter di RSUD DR. Soetomo Surabaya bulan Agustus sampai September 2016 adalah Escherichia coli (20%), Providencia stuartii (14,28%), Klebsiella pneumonia (5,71%), dan Yeast (5,71%). DOI : 10.35990/mk.v5n3.p244-254
Pemeriksaan Pap Smear di lingkungan Universitas Wijaya Kusuma Surabaya sebagai upaya deteksi dini kanker serviks Lusiani Tjandra; Masfufatun Masfufatun; Rini Purbowati; Noer Kumala Indahsari
Jurnal Pengabdian Masyarakat: Humanity and Medicine Vol 4 No 2 (2023): Jurnal Pengabdian Masyarakat: Humanity and Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/Hummed.V4I2.89

Abstract

Based on data from the Global Burden Of Cancer Study (Globocan) in 2020, total cancer cases in Indonesia in 2020 reached 396,914 cases and total deaths of 234,511 cases and cervical cancer (cervix) ranks second with 36,633 cases or 9.2% of total cancer cases. The incidence of cervical cancer will greatly affect the lives of sufferers and their families and will also greatly affect the health financing sector by the government. Therefore, increasing efforts to treat cervical cancer, especially in the field of prevention and early detection, is needed by every party involved. The purpose of this activity is to improve women's reproductive health through Pap smear examination at Wijaya Kusuma University Surabaya as detection of cervical cancer. The webinar activity was held on 18 December 2020 online and the free Pap smear examination was held on 21 April 2021 at the UWKS polyclinic. The result of this activity is that webinar participants can feel how important it is to know early detection of cervical cancer and realize the importance of early detection in reproductive health. At the time of the free Pap smear, the participants received a Pap smear medical examination, the sample was taken by Skin and Genital Specialist and then the sample was examined by Anatomy Pathology Specialist. Next, participants who needed further consultation and treatment were conducted at the FK-UWKS polyclinic.
Co-Authors Agusniar Furkani Listyawati Ayly Soekanto Emilia Devi Dwi Rianti Febtarini Rahmawati Herliani, Olivia I Gede Herry Ananta Wijaya Lusiani Tjandra Masfufatun Masfufatun Nini Primadhani Paras Shinta Dewi Noer Kumala Indahasari Noer Kumala Indahsari Noer Kumala Indahsari, Noer Kumala Sri Lestari Utami Sugiharto