Ari Sri Windyaswari, Ari Sri
Program Studi Farmasi, Fakultas Farmasi, Universitas Jenderal Achmad Yani

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

KAJIAN PENDAHULUAN POTENSI ANTI KANKER DENGAN UJI TOKSISITAS METODE BRINE SHRIMP LETHALITY TEST (BSLT) TERHADAP EKSTRAK ETANOL DAN FRAKSI-FRAKSI DARI KULIT BATANG KEMIRI Aleurites moluccana (L.) Willd. Windyaswari, Ari Sri; Faramayuda, Fahrauk; Ratnasari, Dessy
Kartika Jurnal Ilmiah Farmasi Vol 3, No 1 (2015)
Publisher : Universitas Jenderal Achmad Yani, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (761.757 KB) | DOI: 10.26874/kjif.v3i1.95

Abstract

ABSTRAK Pohon kemiri Aleurites moluccana (L.) Willd.,  adalah tanaman asli Indonesia multiguna, tumbuh menyebar hingga 10-15 meter pada area terbuka di dataran Indonesia Malaysia dan kepulauan Pasifik. Penelitian sebelumnya menunjukkan tanaman kemiri mengandung tanin, saponin, flavonoida dan polifenol.  Penelitian  ini bertujuan untuk mengetahui potensi anti kanker melalui pengujian toksisitas ekstrak etanol kulit batang kemiri Aleurites moluccana (L.) Willd. dengan metode BSLT menggunakan hewan uji larva udang Artemia salina. Pengukuran toksisitas dinyatakan dengan nilai LC50. Pembuatan ekstrak dilakukan dengan teknik maserasi menggunakan pelarut etanol. Hasil pengujian toksisitas ditunjukkan dengan nilai LC50 dari ekstrak etanol, fraksi etil asetat, fraksi n-heksana, dan fraksi air.  Nilai toksisitas yang paling kuat hingga paling lemah adalah fraksi etil asetat, fraksi n-heksan, ekstrak etanol, fraksi air secara berturut-turut adalah 17,10 ppm; 35,74 ppm; 39,29 ppm; dan 378,532 ppm. Intensitas nilai LC50  hasil pengujian BSLT dari fraksi etil asetat, fraksi n-heksan, ekstrak etanol dan fraksi air berturut-turut adalah sangat toksik, sangat toksik, sangat toksik, dan toksik. Hasil Kromatografi Lapis Tipis (KLT) menggunakan penampak bercak spesifik menunjukkan dugaan golongan flavonoid pada ekstrak etanol dan fraksi etil asetat pada kulit batang kemiri. Kata kunci   :  BSLT, kulit batang kemiri, Aleurites moluccana (L.) Willd., flavonoid. ABSTRACT Aleurites moluccana (L.) Willd., also known as candlenut, is a native to Indonesia great domesticated multipurpose trees, typically grows to 10-15 m in open areas,  It is native to the Indo-Malaysia region and was introduced throughout the Pacific Island. Previous studies have demonstrated that the leaves and stem bark of Aleurites moluccana (L.) Willd. presents  tannins, saponin, flavonoid, and polyphenols. The aim of this research was to determine the toxicity value using BSLT method as screening for new potential cytotoxic drug. This research was started with maceration technique using ethanol solvent. The research was started with maceration method using ethanol solvent. Determination of toxicity value of Aleurites molucana (L.) Willd. stem bark were examined using BSLT method. The toxicity value for extract and each fractions was reported as medial lethal concentration (LC50) value, expressed in ppm. The LC50 value using BSLT method of ethyl acetate fraction, n-hexane fraction, ethanol extract, water fraction were 17,10 ppm; 35,74 ppm; 39,39 ppm; 380,93 ppm respectively. While intensity of LC50 value for toxicity value of ethyl acetate fraction, n-hexane fraction, ethanol extract, water fraction were very toxic, very toxic, very toxic, and toxic. Chromatogram pattern were observed by Thin Layer Chromatography (TLC) with specific spray agent, detected the flavonoid in ethanol extract and ethyl acetate fraction from stem bark of Aleurites moluccana (L.) Willd. Key words   : BSLT, stem bark of candlenut, Aleurites moluccana (L.) Willd., flavonoid.
Identification of the Secondary Metabolites and Characterization of Lagerstroemia Loudonii T. & B. Faramayuda, Fahrauk; Hermanto, Faizal; Windyaswari, Ari Sri; Riyanti, Soraya; Nurhayati, Viola Aditya
Journal of Islamic Pharmacy Vol 6, No 1 (2021): J. Islamic Pharm.
Publisher : Universitas Islam Negeri Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jip.v6i1.11351

Abstract

Bungur  (Lagerstroemia loudonii T. B) is a type of plant widely grown in Indonesia and can be found in teak forests, mixed forests, and is found as ornamental plants or protective trees on the roadside. In the fruit section, Lagerstroemia loudonii is used as antituberculous and antimalarial. On the bark, the part is used as antidiarrheal. Based on some parts of the Lagerstroemia loudonii  plants' activity data, this plant has the potential to be developed into traditional medicine. Standardized traditional medicine material is necessary to identify efficacious compounds and characterization in some parts of Lagerstroemia loudonii. The purpose of this research is to develop Lagerstroemia loudonii into traditional herbal medicine or standardized herbal medicine. Identification of efficacious compounds and characterization of crude leaf drugs, bark, stems, and fruit of Lagerstroemia loudonii. The phytochemical screening phase of the crude drugs of leaves, bark, stems, and fruit ofLagerstroemia loudonii against includes examining alkaloids, flavonoids,  quinones, tannins, polyphenols, saponins, steroids and triterpenes, monoterpenoids and sesquiterpenoids. The determination of the characteristics of raw material carried out includes nonspecific parameters. Nonspecific parameters are the determination of total ash content, water-soluble ash content, acid insoluble ash content. each experiment was carried out three times and calculated the average yield and deviation.  Identification results of the class of efficacious compounds in some parts of the Lagerstroemia loudonii  plant are on the leaves and fruits containing alkaloids, flavonoids, saponins, quinones, tannins, polyphenols, monoterpenoids, and sesquiterpenoids as well as steroids and triterpenoids. At the bark and stem, the bark contains alkaloids, flavonoids, saponins, quinones, tannins, polyphenols, monoterpenoids, and sesquiterpenoids. Characterization results of Lagerstroemia loudonii  leaf extract total ash content 4.45 ± 0.30% w/w, water-soluble ash content 4.08 ± 0.27% w/w, acid insoluble ash content 0.59 ± 0.06% w/w, the extract specific gravity was 0.59 ± 0.063. Lagerstroemia loudonii  stem bark extract, total ash content 1.94 ± 0.12% w/w, water-soluble ash content 1.47 ± 0.03% w/w, acid insoluble ash content 0.24 ± 0.02% w/w, the extract specific gravity is 0.82 ± 0.01. Lagerstroemia loudonii  stem extract, total ash content3.18 ± 0.16% w/w, water-soluble ash content 2.36 ± 0.38% w/w, acid insoluble ash content 0.43 ± 0.07% w/w, extract specific grafity 0.81 ± 0.01. Lagerstroemia loudonii  fruit extract, total ash content 11.45 ± 1.16%w/w, water-soluble ash content 10.1 ± 1.49% w/w, acid insoluble ash content 1.46 ± 0.88% w/w,extract specific grafity 0.81 ± 0.01. Based on phytochemical screening data and the characterization of bungur plants potential to be developed into raw materials for traditional medicineKeywords: Lagerstroemia loudonii, secondary metabolite, raw material characterization