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All Journal Pharmascience Window of Health : Jurnal Kesehatan Abdimas Galuh : Jurnal Pengabdian Kepada Masyarakat Hydrogen: Jurnal Kependidikan Kimia Journal Microbiology Science
Selpida Handayani, Selpida
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health

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Journal : Journal Microbiology Science

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Antibacterial Activity of Ethanol Extract of Purple Kencana Leaves (Ruellia tuberosa L.) Using TLC-Bioautography and Agar Diffusion Methods Sari, Felisa Ananda; Herwin, Herwin; Handayani, Selpida
Journal Microbiology Science Vol 3, No 2 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v3i2.982

Abstract

Kencana Ungu Leaf (Ruellia tuberosa L.) is a traditional plant that has antibacterial activity. The purpose of this study was to determine the antibacterial activity of the ethanol extract of Kencana Ungu leaves (Ruellia tuberosa L.) using TLC-Bioautography and Agar Diffusion methods. Simplisia from Kencana Ungu leaves (Ruellia tuberosa L.) was extracted by maceration method and then evaporated to obtain a thick extract. Preliminary testing, namely the antibacterial screening test, active extract at a concentration of 0.5% against Escherichia coli, Shigella dysenteriae, and Salmonella thypi. The results of the antibacterial activity test using the TLC-Bioautography method using chloroform: methanol (7:1) as an eluent showed results with an Rf value of 0.89 which inhibited the growth of Escherichia coli, Shigella dysenteriae, and Salmonella thypi bacteria. While the results of testing the antibacterial activity using the Agar Diffusion method obtained the largest diameter of the inhibition zone at a concentration of 20% for Escherichia coli bacteria with an inhibition zone diameter of 12.15 mm, Shigella dysenteriae with an inhibition zone diameter of 11.72 mm, and Salmonella thypi with an inhibition zone diameter of 12.67 mm
Co-Authors Amran, Farizah Dhaifina Andina, Velda Hasnaeni hasnaeni, Hasnaeni Herwin Herwin, Herwin Hossani, Rahni Indah Pratiwi Nurlina Nurlina Risda Waris, Risda Sari, Felisa Ananda Wisdawati