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All Journal Jurnal Teknologi Laboratorium Health Notions
I Nyoman Jirna, I Nyoman
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Chemistry Dentistry Health Professions Medicine & Pharmacology Nursing Public Health Other

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Potential Pakraman Village in Achieving Bali Rabies Free Jirna, I Nyoman; Sudarmanto, I Gede; Sundari, Cok Dewi Widhya Hana; Arjani, I A Made Sri; Posmaningsih, Dewa Ayu Agustini; Jana, I Wayan; Habibah, Nur
Health Notions Vol 2 No 4 (2018): April 2018
Publisher : Humanistic Network for Science and Technology (Address: Cemara street 25, Ds/Kec Sukorejo, Ponorogo, East Java, Indonesia 63453)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.843 KB)

Abstract

Various ways have been applied to free Bali from the spread of rabies virus. Socialization / counseling to increase knowledge and understanding of the community about rabies and its prevention has been implemented. Rabies vaccination in rabies-transmitted animals has been done, even periodically every year mass rabies vaccinations have been held, and 2015 is the sixth vaccination of rabies. Reduced wild dog populations or elimination have been performed. But rabies never stops, even today all districts in Bali are red zone rabies cases. This study aimed to measure the knowledge level of Pakraman Village leaders and community participation in identifying the potential of Pakraman Village and to analyze the model of community participation in rabies prevention. The subjects were 76 head of family in Tabanan District, Klungkung District, Bangli District, Karangasem District and Buleleng District. This study used pretest and posttest group design, ie a design to determine the impact of the treatment provided by measuring the level of community participation before and after intervention. Data on knowledge was collected by filling out questionnaires by 50 prajuru (administrators) of Pakraman Village before and after being given intervention in the form of counseling with art media of Bondres. Community participation data was collected through interviews before and after intervention. Potential data of Desa Pakraman and the pattern of community participation were collected through in-depth interviews and focus group discussions. Data analysis was done descriptively in the form of central tendency because of numerical data type. Hypothesis testing was done using Wilcoxon signed rank test. Based on the result of the research, it can be concluded that: 1) effective health education to increase knowledge of village administrator and public participation in eradicating rabies virus; 2) pattern / model of community participation in eradication of rabies virus more dominant influenced by visit / suggestion. Furthermore, it is suggested that village administrator pakraman to be more intent to make visits / suggestions to educate the society so that community participation is more increased. In addition it also needs mentoring from the government in educating the community so that more effective coaching.
Isolasi Candida albicans Dari Swab Mukosa Mulut Penderita Diabetes Melitus Tipe 2 Kadek Sri Jayanti, Ni; Jirna, I Nyoman
Jurnal Teknologi Laboratorium Vol 7 No 1 (2018): 2018 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (279.621 KB) | DOI: 10.29238/teknolabjournal.v7i1.103

Abstract

Candida albicans can be pathogen when immunity had decreased and physiological function is impaired, such as in diabetes mellitus type 2. This study aims to isolation Candida albicans that collected from the oral cavity of diabetes mellitus type 2 patients. This research is conducted with a descriptive study by observing the presence of Candida albicans in 30 samples of diabetes mellitus type 2 patients, which grows on Potato Dextrose Agar. The microscopic observation by LPCB staining of yeasts, blastospores, pseudohyphae, chlamydospores and germ tubes in human serum suspension that incubated at 370C for 2-3 hours. Based on this research was found 14 (46,7%) patients from 30 patients were positive Candida albicans.
The potential of traditional balinese spices against the growth of Salmonella sp in vitro Jirna, I Nyoman; Sudarmanto, I Gede; Kurniawan, Surya Bayu; Ratih, Gusti Ayu Made; Rasyid, Burhannuddin
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i1.200

Abstract

Prevention by utilizing medicinal plants can be used as an alternative treatment. This study aims to identify active antimicrobial substances and test the antimicrobial potential of traditional Balinese spices, namely Basa Selem, Basa Gede, Basa Wangen, Basa Bawang Jahe, and Basa Rajang against Salmonella sp bacteria in vitro. The True-experimental method with Posttest only-control design was used in this study, by intervening in the treatment group as well as the presence of positive and negative controls. The maceration method with 96% EtOH solvent was used to extract active substances and identify the levels of antimicrobial active substances. The TLC Spectrophotodensitometer instrument and the diffusion method (discs) were used to test antimicrobial potential. Data analysis was performed using the one-way ANOVA test. All five samples showed flavonoids, alkaloids, tannins, and phenols in qualitative tests. Based on quantitative test results of five samples, the highest compound content obtained in Basa Wangen (6.66 mg/ml of tannins), Basa Gede (3.74 mg/ml of flavonoids), Basa Bawang Jahe (2.49 mg/ml of tannins), Basa Selem (2.87 mg/ml of tannin), and Basa Rajang (6.96 mg/ml of flavonoids). There are differences in the antimicrobial potential of various types of traditional Balinese spices against the growth of Salmonella sp in vitro (sig = 0.037). The traditional Balinese spices have the antimicrobial potential of the intermediate category with a range of inhibition (16-20 mm) based on the NCCLS standard.
Co-Authors Arjani, I A Made Sri Burhannuddin Cokorda Dewi Widhya Hana Sundari Dewa Ayu Agustini Posmaningsih I Wayan Jana Kadek Sri Jayanti, Ni Kurniawan, Surya Bayu Nur Habibah Ratih, Gusti Ayu Made Sudarmanto, I Gede Sudarmanto, I Gede