Article Per Year (5 Year)
p-Index From 2019 - 2024
0
P-Index
This Author published in this journals
All Journal JURNAL KIMIA SAINS DAN APLIKASI
Mutmainah Mutmainah
Sekolah Tinggi Ilmu Farmasi Semarang
Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Validation of UV-VIS Spectrophotometric Methods for Determination of Inulin Levels from Lesser Yam (Dioscorea esculenta L.) Sandra Ayu Apriliyani; Yohanes Martono; Cucun Alep Riyanto; Mutmainah Mutmainah; Kusmita Kusmita
Jurnal Kimia Sains dan Aplikasi Vol 21, No 4 (2018): volume 21 Issue 4 Year 2018
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2211.725 KB) | DOI: 10.14710/jksa.21.4.161-165

Abstract

Validation of methods is necessary to ensure that the analytical methods used are appropriate and reliable. In this research, the determination of validation method and determination of inulin content from gembili bulb (Dioscorea esculenta L.). The method used for determining inulin levels is UV-Vis spectrophotometry. Based on the results obtained, the maximum wavelength determination of inulin levels is 520 nm. The method of analysis in this study meets the specified validation requirements. These parameters include the equation on the inulin raw curve y = 0.0045x +0.109 with the coefficient of determination 0.9992. The accuracy of the methods tested using the recovery test was 96.14-106.76% at concentrations of 25, 100, and 225 ppm. The intraday precision test is in the range of 2.67-6.82% at the same concentration as the accuracy test. Precision interday equal to 7.41% at concentration 225 ppm for 3 day analysis period. The values of LOD and LOQ are 3.58 ppm and 11.95 ppm, respectively. The concentration of inulin extract from gembili bulb is 840.44-12188.59 ppm.
Co-Authors Cucun Alep Riyanto Kusmita Kusmita Sandra Ayu Apriliyani Yohanes Martono