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All Journal International Journal of Cell and Biomedical Science
Awik P.D. Nurhayati
Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Surabaya 60111, Indonesia
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Trisindoline 5 Compound Inhibits Human Breast Cancer Stem Cell Formation by Downregulating the BCL-2 Gene Expression Muhammad Fatoni; Yofinta I Salsabila; Awik P.D. Nurhayati; Shabrina Syifa Ghaissani; Mardi Santoso; Fahimah Martak
International Journal of Cell and Biomedical Science Vol 1 No 2 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i2.19

Abstract

Background: Breast cancer cell's growth and survival are supported by breast cancer stem cells (bCSCs) mammosphere formation. bCSCs represent a subpopulation of undifferentiated cancer cells which associated with self-renewing abilities, tumor initiation, drug resistance, and metastasis. Overexpression of the B Cell Lymphoma 2 (BCL-2) family in many tumor cells contributes to apoptosis resistance. Trisindoline is an indole trimer alkaloid natural compound that provides a cytotoxic effect on cancer cells. A new modification of trisindoline has been synthesized into trisindoline 5 in 2021. Objective: This study purposed to investigate the effect of trisindoline 5 compounds against BCL-2 gene expression in bCSCs in vitro. Methods: The bCSCs MDA-MB-231 were divided into control and treatment groups which were further analyzed in gene expression using the qPCR Livak method. Results: Based on gene expression analysis, the results showed that trisindoline 5 may decrease the expression of BCL-2 in MDA-MB-231 cells. Conclusion: This study concludes that trisindoline 5 could downregulate the antiapoptotic BCL-2 gene expression in bCSCs in vitro.
Co-Authors Fahimah Martak Mardi Santoso Muhammad Fatoni Shabrina Syifa Ghaissani Yofinta I Salsabila