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OPTIMASI EFISIENSI FLOKULASI PADA PROSES PANEN MIKROALGA POTENSIAL PENGHASIL BIODIESEL DENGAN FLOKULAN ION MAGNESIUM - (OPTIMIZATION OF FLOCCULATION EFFICIENCY IN THE HARVESTING PROCESS OF POTENTIAL BIODIESEL PRODUCING MICROALGAE BY USING MAGNESIUM IONS) Praharyawan, Swastika; Putri, Silviredeta Anindya
Biopropal Industri Vol 8, No 2 (2017)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.105 KB)

Abstract

Flocculation is a feasible method for harvesting microalgae due to its lower cost. This research aims to optimize the flocculation efficiency in harvesting biodiesel-producing microalgae. Flocculation process was conducted towards microalgae cell suspension on 0.2-1 g/L range concentration by applying magnesium ions (1 until 5 mM) at alkaline condition (pH 9-12) in 30 and 60 minutes using neutralization and sweeping. The result showed that magnesium ion concentration, pH value and biomass concentration had significant effect on the efficiency of microalgae cells flocculation, while flocculation time did not show significant effect. Flocculation efficiency increased when magnesium ion concentration was 4 mM and started at pH 10 for microalgae culture with high biomass concentration and at pH 11.5 for low biomass concentration, while optimum pH for both culture was 11.75. Flocculation efficiency for microalgae with high concentration of biomass at optimum condition was 94.89% while for the low one was 89.75%.Keywords: flocculation, flocculation efficiency, magnesium ions, microalgaeABSTRAKMikroalga berpotensi sebagai bahan baku biodiesel. Metode flokulasi layak untuk diterapkan pada pemanenan mikroalga karena berbiaya rendah. Penelitian ini bertujuan untuk mengoptimasi efisiensi flokulasi pada proses pemanenan mikroalga lokal potensial penghasil biodiesel yang menggunakan metode otoflokulasi di bawah pengaruh nilai pH, konsentrasi ion magnesium, konsentrasi biomassa dan waktu flokulasi. Proses flokulasi dilakukan pada suspensi sel mikroalga konsentrasi 0,2 hingga 1 g/L dengan menggunakan ion magnesium (1 hingga 5 mM) pada suasana basa (pH 9-12) selama waktu tertentu (30 dan 60 menit) melalui mekanisme netralisasi muatan dan penyapuan (sweeping). Hasil penelitian menunjukkan bahwa konsentrasi ion magnesium, nilai pH dan konsentrasi biomassa memberikan pengaruh yang signifikan terhadap efisiensi flokulasi sel-sel mikroalga sedangkan waktu flokulasi tidak menunjukkan pengaruh signifikan. Efisiensi flokulasi meningkat secara signifikan pada konsentrasi ion magnesium hingga 4 mM dan dimulai pada pH 10 untuk kultur mikroalga dengan konsentrasi biomassa tinggi (1 g/L) dan pada pH 11,5 untuk kultur dengan konsentrasi biomassa rendah (0,2 g/L) sedangkan pH optimum untuk kedua kultur adalah 11,75. Nilai efisiensi flokulasi untuk kultur mikroalga dengan konsentrasi biomassa tinggi pada kondisi optimum mencapai 94,89%, sementara nilai efisiensi flokulasi untuk kultur dengan konsentrasi biomassa rendah adalah 89,75%.Kata kunci: efisiensi flokulasi, flokulasi, ion magnesium, mikroalga
Morphology and molecular characterization of newly isolated microalgae strain Chlorella volutis LIPI13-WKT066 from Wakatobi Islands and its potential use Rahman, Delicia Yunita; Praharyawan, Swastika; Raharjo, Sapto; Fadiyah, Farizul; Susilaningsih, Dwi
ANNALES BOGORIENSES Vol 23, No 1 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2019.v23.n1.13-19

Abstract

Morphology and molecular characterization of microalgae isolated from Wakatobi Marine National Park was conducted. An understanding of the characteristics of morphology, molecular, as well as metabolites profile of the microalgae species is potentially useful for its future application. The primary aim of this study was to isolate, identify and characterize the microalgae strain isolated from Wakatobi Marine National Park labeled as LIPI13-WKT066 with the emphasis on the evaluation of amino acid content as a basis for its health application. Morphological observation under the microscope and molecular identification suggested that the microalgae strain of LIPI13-WKT066 belong to the strain under species of Chlorella volutis. Metabolite characterization of the microalgae strain showed that the content of protein (11.9%), lipid (12.4%) and carbohydrate (4.7%) was in the regular range. Further analysis of its amino acid content revealed the potency of the microalgae strain to be used as antihypertensive agent.
Characterization of lipid productivity and fatty acid profile of three fast-growing microalgae isolated from Bengkulu for possible use in health application Praharyawan, Swastika; Rahman, Delicia Yunita; Susilaningsih, Dwi
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.03

Abstract

Three strains of fast-growing microalgae were investigated in this study for their potential of lipid production and its possible use in health application. Lipid content, lipid productivity, and fatty acids profile of the 3 mi-croalgae strains were also analyzed. The strain of LBB13-2-AL045 and LBB13-2-AL048 possessed highest lipid content (49.08 ± 0.25%) and lipid productivity (40.27 ± 1.91 mg.L-1.day-1), respectively, among the other tested strains. The fatty acids profile from the 3 strains exhibited its possible use in health application. The two strains of LBB13-2-AL046 and LBB13-2-AL048 possessed high valuable fatty acids of polyunsaturated fatty ac-ids (PUFA) omega-3 and omega-6, whereas LBB13-2- AL045 possessed balance ratio of saturated fatty acid (SFA) : monounsaturated fatty acid (MUFA) : PUFA (1 : 1.3 : 1) as is recommended by American Heart As-sociation (AHA) (1 : 1.4 : 0.8). The high content of omega-3 and omega-6 fatty acids indicated that the algal lipids of two strains (LBB13-2-AL046 and LBB13-2-AL048) were potential to be applied in cardiovascular health. The balance ratio of SFA : MUFA: PUFA as is recommended by AHA indicated that the algal oil of LBB13-2-AL045 strain was recommended in order to generate the best LDL/HDL ratio.